5LPP
tRNA guanine Transglycosylase (TGT) in co-crystallized complex (space group C2) with 6-amino-4-(2-((3aR,4R,6R,6aR)-6-methoxy-2,2-dimethyltetrahydrofuro[3,4-d][1,3]dioxol-4-yl)ethyl)-2-(methylamino)-1H-imidazo[4,5-g]quinazolin-8(7H)-one
Functional Information from GO Data
Chain | GOid | namespace | contents |
A | 0002099 | biological_process | tRNA wobble guanine modification |
A | 0005737 | cellular_component | cytoplasm |
A | 0005829 | cellular_component | cytosol |
A | 0006400 | biological_process | tRNA modification |
A | 0008033 | biological_process | tRNA processing |
A | 0008479 | molecular_function | tRNA-guanosine(34) queuine transglycosylase activity |
A | 0008616 | biological_process | queuosine biosynthetic process |
A | 0016757 | molecular_function | glycosyltransferase activity |
A | 0016763 | molecular_function | pentosyltransferase activity |
A | 0046872 | molecular_function | metal ion binding |
A | 0101030 | biological_process | tRNA-guanine transglycosylation |
Functional Information from PDB Data
site_id | AC1 |
Number of Residues | 4 |
Details | binding site for residue ZN A 401 |
Chain | Residue |
A | CYS318 |
A | CYS320 |
A | CYS323 |
A | HIS349 |
site_id | AC2 |
Number of Residues | 16 |
Details | binding site for residue 72C A 402 |
Chain | Residue |
A | CYS158 |
A | ILE201 |
A | GLN203 |
A | GLY229 |
A | GLY230 |
A | LEU231 |
A | ALA232 |
A | MET260 |
A | GLY261 |
A | ASP280 |
A | HOH589 |
A | LEU68 |
A | ASP102 |
A | SER103 |
A | TYR106 |
A | ASP156 |
Functional Information from SwissProt/UniProt
Chain | Residue | Details |
A | ASP102 | |
Chain | Residue | Details |
A | ASP280 | |
Chain | Residue | Details |
A | ASP102 | |
A | ASP156 | |
A | GLN203 | |
A | GLY230 | |
site_id | SWS_FT_FI4 |
Number of Residues | 4 |
Details | BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_00168, ECO:0000269|PubMed:10413112, ECO:0000269|PubMed:11178905, ECO:0000269|PubMed:11921407, ECO:0000269|PubMed:12646024, ECO:0000269|PubMed:12909636, ECO:0000269|PubMed:12949492, ECO:0000269|PubMed:14523925, ECO:0000269|PubMed:19627989, ECO:0000269|PubMed:8654383, ECO:0000269|PubMed:8961936 |
Chain | Residue | Details |
A | CYS318 | |
A | CYS320 | |
A | CYS323 | |
A | HIS349 | |
Catalytic Information from CSA
site_id | MCSA1 |
Number of Residues | 6 |
Details | M-CSA 881 |
Chain | Residue | Details |
A | ASP102 | proton shuttle (general acid/base) |
A | ASP280 | covalent catalysis |
A | CYS318 | metal ligand |
A | CYS320 | metal ligand |
A | CYS323 | metal ligand |
A | HIS349 | metal ligand |