5LPO

tRNA guanine Transglycosylase (TGT) in co-crystallized complex with 6-amino-2-(methylamino)-4-(2-((2R,3R,4R,5R)-3,4,5-trimethoxytetrahydrofuran-2-yl)ethyl)-1H-imidazo[4,5-g]quinazolin-8(7H)-one

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0002099	biological_process	tRNA wobble guanine modification
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0006400	biological_process	tRNA modification
A	0008033	biological_process	tRNA processing
A	0008479	molecular_function	tRNA-guanosine(34) queuine transglycosylase activity
A	0008616	biological_process	queuosine biosynthetic process
A	0016757	molecular_function	glycosyltransferase activity
A	0016763	molecular_function	pentosyltransferase activity
A	0046872	molecular_function	metal ion binding
A	0101030	biological_process	tRNA-guanine transglycosylation

Functional Information from PDB Data

site_id	AC1
Number of Residues	7
Details	binding site for residue GOL A 401

Chain	Residue
A	GLY46
A	ALA49
A	VAL51
A	MET93
A	TYR330
A	LEU341
A	HOH536

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site_id	AC2
Number of Residues	7
Details	binding site for residue GOL A 402

Chain	Residue
A	ARG174
A	PRO252
A	ASP254
A	LYS255
A	HOH524
A	GLN117
A	SER118

---

site_id	AC3
Number of Residues	9
Details	binding site for residue GOL A 403

Chain	Residue
A	THR27
A	GLY28
A	THR29
A	ARG38
A	THR39
A	ARG362
A	SER366
A	GOL404
A	HOH510

---

site_id	AC4
Number of Residues	7
Details	binding site for residue GOL A 404

Chain	Residue
A	ASN304
A	GLN359
A	ARG362
A	ASP363
A	SER366
A	GOL403
A	HOH604

---

site_id	AC5
Number of Residues	7
Details	binding site for residue GOL A 405

Chain	Residue
A	HIS145
A	LEU146
A	SER188
A	ARG189
A	LYS190
A	HOH576
A	HOH594

---

site_id	AC6
Number of Residues	4
Details	binding site for residue ZN A 406

Chain	Residue
A	CYS318
A	CYS320
A	CYS323
A	HIS349

---

site_id	AC7
Number of Residues	18
Details	binding site for residue 725 A 407

Chain	Residue
A	LEU100
A	ASP102
A	SER103
A	TYR106
A	ASP156
A	CYS158
A	ILE201
A	GLN203
A	GLY229
A	GLY230
A	LEU231
A	ALA232
A	TYR258
A	MET260
A	GLY261
A	ASP280
A	HOH542
A	HOH585

---

site_id	AC8
Number of Residues	6
Details	binding site for residue EDO A 408

Chain	Residue
A	ARG34
A	HIS145
A	SER149
A	HOH529
A	HOH547
A	HOH550

---

site_id	AC9
Number of Residues	11
Details	binding site for residue PG4 A 409

Chain	Residue
A	SER15
A	PHE16
A	SER17
A	ILE18
A	GLU119
A	ARG174
A	ARG177
A	ASP254
A	LYS255
A	HOH525
A	HOH737

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1
Details	ACT_SITE: Proton acceptor => ECO:0000255|HAMAP-Rule:MF_00168, ECO:0000305|PubMed:12949492

Chain	Residue	Details
A	ASP102

---

site_id	SWS_FT_FI2
Number of Residues	1
Details	ACT_SITE: Nucleophile => ECO:0000255|HAMAP-Rule:MF_00168, ECO:0000305|PubMed:12949492

Chain	Residue	Details
A	ASP280

---

site_id	SWS_FT_FI3
Number of Residues	4
Details	BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_00168, ECO:0000269|PubMed:12949492

Chain	Residue	Details
A	GLY230
A	ASP102
A	ASP156
A	GLN203

---

site_id	SWS_FT_FI4
Number of Residues	4
Details	BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_00168, ECO:0000269|PubMed:10413112, ECO:0000269|PubMed:11178905, ECO:0000269|PubMed:11921407, ECO:0000269|PubMed:12646024, ECO:0000269|PubMed:12909636, ECO:0000269|PubMed:12949492, ECO:0000269|PubMed:14523925, ECO:0000269|PubMed:19627989, ECO:0000269|PubMed:8654383, ECO:0000269|PubMed:8961936

Chain	Residue	Details
A	CYS320
A	CYS323
A	HIS349
A	CYS318

---

Catalytic Information from CSA

site_id	MCSA1
Number of Residues	6
Details	M-CSA 881

Chain	Residue	Details
A	ASP102	proton shuttle (general acid/base)
A	ASP280	covalent catalysis
A	CYS318	metal ligand
A	CYS320	metal ligand
A	CYS323	metal ligand
A	HIS349	metal ligand

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