Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

5LF0

Human 20S proteasome complex with Epoxomicin at 2.4 Angstrom

Functional Information from GO Data

Chain	GOid	namespace	contents
a	0005839	cellular_component	proteasome core complex
a	0051603	biological_process	proteolysis involved in protein catabolic process
A	0000502	cellular_component	proteasome complex
A	0000932	cellular_component	P-body
A	0005515	molecular_function	protein binding
A	0005576	cellular_component	extracellular region
A	0005634	cellular_component	nucleus
A	0005654	cellular_component	nucleoplasm
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0005839	cellular_component	proteasome core complex
A	0006511	biological_process	ubiquitin-dependent protein catabolic process
A	0009615	biological_process	response to virus
A	0019773	cellular_component	proteasome core complex, alpha-subunit complex
A	0034774	cellular_component	secretory granule lumen
A	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
A	0051603	biological_process	proteolysis involved in protein catabolic process
A	0070062	cellular_component	extracellular exosome
A	1904813	cellular_component	ficolin-1-rich granule lumen
b	0004298	molecular_function	threonine-type endopeptidase activity
b	0005839	cellular_component	proteasome core complex
b	0051603	biological_process	proteolysis involved in protein catabolic process
B	0000502	cellular_component	proteasome complex
B	0000932	cellular_component	P-body
B	0005515	molecular_function	protein binding
B	0005634	cellular_component	nucleus
B	0005654	cellular_component	nucleoplasm
B	0005737	cellular_component	cytoplasm
B	0005829	cellular_component	cytosol
B	0005839	cellular_component	proteasome core complex
B	0006511	biological_process	ubiquitin-dependent protein catabolic process
B	0019773	cellular_component	proteasome core complex, alpha-subunit complex
B	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
B	0043231	cellular_component	intracellular membrane-bounded organelle
B	0051603	biological_process	proteolysis involved in protein catabolic process
B	0070062	cellular_component	extracellular exosome
C	0000502	cellular_component	proteasome complex
C	0005515	molecular_function	protein binding
C	0005634	cellular_component	nucleus
C	0005654	cellular_component	nucleoplasm
C	0005737	cellular_component	cytoplasm
C	0005829	cellular_component	cytosol
C	0005839	cellular_component	proteasome core complex
C	0006511	biological_process	ubiquitin-dependent protein catabolic process
C	0019773	cellular_component	proteasome core complex, alpha-subunit complex
C	0042802	molecular_function	identical protein binding
C	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
C	0051603	biological_process	proteolysis involved in protein catabolic process
C	0070062	cellular_component	extracellular exosome
C	0098794	cellular_component	postsynapse
D	0000502	cellular_component	proteasome complex
D	0005515	molecular_function	protein binding
D	0005576	cellular_component	extracellular region
D	0005634	cellular_component	nucleus
D	0005654	cellular_component	nucleoplasm
D	0005737	cellular_component	cytoplasm
D	0005829	cellular_component	cytosol
D	0005839	cellular_component	proteasome core complex
D	0006511	biological_process	ubiquitin-dependent protein catabolic process
D	0019773	cellular_component	proteasome core complex, alpha-subunit complex
D	0034774	cellular_component	secretory granule lumen
D	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
D	0051603	biological_process	proteolysis involved in protein catabolic process
D	0070062	cellular_component	extracellular exosome
D	1904813	cellular_component	ficolin-1-rich granule lumen
E	0000502	cellular_component	proteasome complex
E	0001530	molecular_function	lipopolysaccharide binding
E	0002376	biological_process	immune system process
E	0002862	biological_process	negative regulation of inflammatory response to antigenic stimulus
E	0005515	molecular_function	protein binding
E	0005634	cellular_component	nucleus
E	0005654	cellular_component	nucleoplasm
E	0005737	cellular_component	cytoplasm
E	0005813	cellular_component	centrosome
E	0005829	cellular_component	cytosol
E	0005839	cellular_component	proteasome core complex
E	0006511	biological_process	ubiquitin-dependent protein catabolic process
E	0019773	cellular_component	proteasome core complex, alpha-subunit complex
E	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
E	0051603	biological_process	proteolysis involved in protein catabolic process
E	0070062	cellular_component	extracellular exosome
F	0000502	cellular_component	proteasome complex
F	0005515	molecular_function	protein binding
F	0005634	cellular_component	nucleus
F	0005654	cellular_component	nucleoplasm
F	0005737	cellular_component	cytoplasm
F	0005829	cellular_component	cytosol
F	0005839	cellular_component	proteasome core complex
F	0006511	biological_process	ubiquitin-dependent protein catabolic process
F	0019773	cellular_component	proteasome core complex, alpha-subunit complex
F	0031625	molecular_function	ubiquitin protein ligase binding
F	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
F	0045202	cellular_component	synapse
F	0051603	biological_process	proteolysis involved in protein catabolic process
F	0052548	biological_process	regulation of endopeptidase activity
F	0070062	cellular_component	extracellular exosome
G	0000502	cellular_component	proteasome complex
G	0000932	cellular_component	P-body
G	0003723	molecular_function	RNA binding
G	0004175	molecular_function	endopeptidase activity
G	0005515	molecular_function	protein binding
G	0005634	cellular_component	nucleus
G	0005654	cellular_component	nucleoplasm
G	0005737	cellular_component	cytoplasm
G	0005829	cellular_component	cytosol
G	0005839	cellular_component	proteasome core complex
G	0005840	cellular_component	ribosome
G	0006511	biological_process	ubiquitin-dependent protein catabolic process
G	0016363	cellular_component	nuclear matrix
G	0019773	cellular_component	proteasome core complex, alpha-subunit complex
G	0030016	cellular_component	myofibril
G	0030017	cellular_component	sarcomere
G	0035639	molecular_function	purine ribonucleoside triphosphate binding
G	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
G	0050727	biological_process	regulation of inflammatory response
G	0051059	molecular_function	NF-kappaB binding
G	0051092	biological_process	positive regulation of NF-kappaB transcription factor activity
G	0051603	biological_process	proteolysis involved in protein catabolic process
G	0070062	cellular_component	extracellular exosome
H	0004298	molecular_function	threonine-type endopeptidase activity
H	0005839	cellular_component	proteasome core complex
H	0051603	biological_process	proteolysis involved in protein catabolic process
I	0000502	cellular_component	proteasome complex
I	0005515	molecular_function	protein binding
I	0005634	cellular_component	nucleus
I	0005654	cellular_component	nucleoplasm
I	0005737	cellular_component	cytoplasm
I	0005829	cellular_component	cytosol
I	0005839	cellular_component	proteasome core complex
I	0019774	cellular_component	proteasome core complex, beta-subunit complex
I	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
I	0051603	biological_process	proteolysis involved in protein catabolic process
I	0070062	cellular_component	extracellular exosome
J	0000502	cellular_component	proteasome complex
J	0005515	molecular_function	protein binding
J	0005634	cellular_component	nucleus
J	0005654	cellular_component	nucleoplasm
J	0005737	cellular_component	cytoplasm
J	0005829	cellular_component	cytosol
J	0005839	cellular_component	proteasome core complex
J	0010243	biological_process	response to organonitrogen compound
J	0010498	biological_process	proteasomal protein catabolic process
J	0014070	biological_process	response to organic cyclic compound
J	0016020	cellular_component	membrane
J	0019774	cellular_component	proteasome core complex, beta-subunit complex
J	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
J	0051603	biological_process	proteolysis involved in protein catabolic process
J	0070062	cellular_component	extracellular exosome
K	0004298	molecular_function	threonine-type endopeptidase activity
K	0005839	cellular_component	proteasome core complex
K	0051603	biological_process	proteolysis involved in protein catabolic process
L	0005839	cellular_component	proteasome core complex
L	0051603	biological_process	proteolysis involved in protein catabolic process
M	0005839	cellular_component	proteasome core complex
M	0051603	biological_process	proteolysis involved in protein catabolic process
N	0004298	molecular_function	threonine-type endopeptidase activity
N	0005839	cellular_component	proteasome core complex
N	0051603	biological_process	proteolysis involved in protein catabolic process
O	0000502	cellular_component	proteasome complex
O	0000932	cellular_component	P-body
O	0005515	molecular_function	protein binding
O	0005576	cellular_component	extracellular region
O	0005634	cellular_component	nucleus
O	0005654	cellular_component	nucleoplasm
O	0005737	cellular_component	cytoplasm
O	0005829	cellular_component	cytosol
O	0005839	cellular_component	proteasome core complex
O	0006511	biological_process	ubiquitin-dependent protein catabolic process
O	0009615	biological_process	response to virus
O	0019773	cellular_component	proteasome core complex, alpha-subunit complex
O	0034774	cellular_component	secretory granule lumen
O	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
O	0051603	biological_process	proteolysis involved in protein catabolic process
O	0070062	cellular_component	extracellular exosome
O	1904813	cellular_component	ficolin-1-rich granule lumen
P	0000502	cellular_component	proteasome complex
P	0000932	cellular_component	P-body
P	0005515	molecular_function	protein binding
P	0005634	cellular_component	nucleus
P	0005654	cellular_component	nucleoplasm
P	0005737	cellular_component	cytoplasm
P	0005829	cellular_component	cytosol
P	0005839	cellular_component	proteasome core complex
P	0006511	biological_process	ubiquitin-dependent protein catabolic process
P	0019773	cellular_component	proteasome core complex, alpha-subunit complex
P	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
P	0043231	cellular_component	intracellular membrane-bounded organelle
P	0051603	biological_process	proteolysis involved in protein catabolic process
P	0070062	cellular_component	extracellular exosome
Q	0000502	cellular_component	proteasome complex
Q	0005515	molecular_function	protein binding
Q	0005634	cellular_component	nucleus
Q	0005654	cellular_component	nucleoplasm
Q	0005737	cellular_component	cytoplasm
Q	0005829	cellular_component	cytosol
Q	0005839	cellular_component	proteasome core complex
Q	0006511	biological_process	ubiquitin-dependent protein catabolic process
Q	0019773	cellular_component	proteasome core complex, alpha-subunit complex
Q	0042802	molecular_function	identical protein binding
Q	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
Q	0051603	biological_process	proteolysis involved in protein catabolic process
Q	0070062	cellular_component	extracellular exosome
Q	0098794	cellular_component	postsynapse
R	0000502	cellular_component	proteasome complex
R	0005515	molecular_function	protein binding
R	0005576	cellular_component	extracellular region
R	0005634	cellular_component	nucleus
R	0005654	cellular_component	nucleoplasm
R	0005737	cellular_component	cytoplasm
R	0005829	cellular_component	cytosol
R	0005839	cellular_component	proteasome core complex
R	0006511	biological_process	ubiquitin-dependent protein catabolic process
R	0019773	cellular_component	proteasome core complex, alpha-subunit complex
R	0034774	cellular_component	secretory granule lumen
R	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
R	0051603	biological_process	proteolysis involved in protein catabolic process
R	0070062	cellular_component	extracellular exosome
R	1904813	cellular_component	ficolin-1-rich granule lumen
S	0000502	cellular_component	proteasome complex
S	0001530	molecular_function	lipopolysaccharide binding
S	0002376	biological_process	immune system process
S	0002862	biological_process	negative regulation of inflammatory response to antigenic stimulus
S	0005515	molecular_function	protein binding
S	0005634	cellular_component	nucleus
S	0005654	cellular_component	nucleoplasm
S	0005737	cellular_component	cytoplasm
S	0005813	cellular_component	centrosome
S	0005829	cellular_component	cytosol
S	0005839	cellular_component	proteasome core complex
S	0006511	biological_process	ubiquitin-dependent protein catabolic process
S	0019773	cellular_component	proteasome core complex, alpha-subunit complex
S	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
S	0051603	biological_process	proteolysis involved in protein catabolic process
S	0070062	cellular_component	extracellular exosome
T	0000502	cellular_component	proteasome complex
T	0005515	molecular_function	protein binding
T	0005634	cellular_component	nucleus
T	0005654	cellular_component	nucleoplasm
T	0005737	cellular_component	cytoplasm
T	0005829	cellular_component	cytosol
T	0005839	cellular_component	proteasome core complex
T	0006511	biological_process	ubiquitin-dependent protein catabolic process
T	0019773	cellular_component	proteasome core complex, alpha-subunit complex
T	0031625	molecular_function	ubiquitin protein ligase binding
T	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
T	0045202	cellular_component	synapse
T	0051603	biological_process	proteolysis involved in protein catabolic process
T	0052548	biological_process	regulation of endopeptidase activity
T	0070062	cellular_component	extracellular exosome
U	0000502	cellular_component	proteasome complex
U	0000932	cellular_component	P-body
U	0003723	molecular_function	RNA binding
U	0004175	molecular_function	endopeptidase activity
U	0005515	molecular_function	protein binding
U	0005634	cellular_component	nucleus
U	0005654	cellular_component	nucleoplasm
U	0005737	cellular_component	cytoplasm
U	0005829	cellular_component	cytosol
U	0005839	cellular_component	proteasome core complex
U	0005840	cellular_component	ribosome
U	0006511	biological_process	ubiquitin-dependent protein catabolic process
U	0016363	cellular_component	nuclear matrix
U	0019773	cellular_component	proteasome core complex, alpha-subunit complex
U	0030016	cellular_component	myofibril
U	0030017	cellular_component	sarcomere
U	0035639	molecular_function	purine ribonucleoside triphosphate binding
U	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
U	0050727	biological_process	regulation of inflammatory response
U	0051059	molecular_function	NF-kappaB binding
U	0051092	biological_process	positive regulation of NF-kappaB transcription factor activity
U	0051603	biological_process	proteolysis involved in protein catabolic process
U	0070062	cellular_component	extracellular exosome
V	0004298	molecular_function	threonine-type endopeptidase activity
V	0005839	cellular_component	proteasome core complex
V	0051603	biological_process	proteolysis involved in protein catabolic process
W	0000502	cellular_component	proteasome complex
W	0005515	molecular_function	protein binding
W	0005634	cellular_component	nucleus
W	0005654	cellular_component	nucleoplasm
W	0005737	cellular_component	cytoplasm
W	0005829	cellular_component	cytosol
W	0005839	cellular_component	proteasome core complex
W	0019774	cellular_component	proteasome core complex, beta-subunit complex
W	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
W	0051603	biological_process	proteolysis involved in protein catabolic process
W	0070062	cellular_component	extracellular exosome
X	0000502	cellular_component	proteasome complex
X	0005515	molecular_function	protein binding
X	0005634	cellular_component	nucleus
X	0005654	cellular_component	nucleoplasm
X	0005737	cellular_component	cytoplasm
X	0005829	cellular_component	cytosol
X	0005839	cellular_component	proteasome core complex
X	0010243	biological_process	response to organonitrogen compound
X	0010498	biological_process	proteasomal protein catabolic process
X	0014070	biological_process	response to organic cyclic compound
X	0016020	cellular_component	membrane
X	0019774	cellular_component	proteasome core complex, beta-subunit complex
X	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
X	0051603	biological_process	proteolysis involved in protein catabolic process
X	0070062	cellular_component	extracellular exosome
Y	0004298	molecular_function	threonine-type endopeptidase activity
Y	0005839	cellular_component	proteasome core complex
Y	0051603	biological_process	proteolysis involved in protein catabolic process
Z	0005839	cellular_component	proteasome core complex
Z	0051603	biological_process	proteolysis involved in protein catabolic process

Functional Information from PDB Data

site_id	AC1
Number of Residues	3
Details	binding site for residue CL A 301

Chain	Residue
A	SER6
C	ARG5
C	HOH405

site_id	AC2
Number of Residues	1
Details	binding site for residue CL A 302

Chain	Residue
A	ARG219

site_id	AC3
Number of Residues	3
Details	binding site for residue CL A 303

Chain	Residue
A	HIS87
A	ARG90
G	LYS116

site_id	AC4
Number of Residues	2
Details	binding site for residue CL A 304

Chain	Residue
A	THR68
A	HOH441

site_id	AC5
Number of Residues	4
Details	binding site for residue CL B 301

Chain	Residue
B	GLU70
B	GLN95
B	CL302
B	HOH491

site_id	AC6
Number of Residues	3
Details	binding site for residue CL B 302

Chain	Residue
B	ASN69
B	CL301
B	HOH414

site_id	AC7
Number of Residues	3
Details	binding site for residue CL C 301

Chain	Residue
C	VAL26
C	ILE161
C	GLY162

site_id	AC8
Number of Residues	3
Details	binding site for residue CL C 302

Chain	Residue
C	ARG86
C	ARG117
C	HOH404

site_id	AC9
Number of Residues	3
Details	binding site for residue CL D 301

Chain	Residue
D	ILE31
D	GLY171
D	SER174

site_id	AD1
Number of Residues	5
Details	binding site for residue CL E 301

Chain	Residue
E	6V1148
E	ILE161
E	GLY162
E	SER165
E	HOH493

site_id	AD2
Number of Residues	2
Details	binding site for residue CL E 302

Chain	Residue
E	6V1148
E	SER150

site_id	AD3
Number of Residues	2
Details	binding site for residue CL E 303

Chain	Residue
D	THR161
E	LEU77

site_id	AD4
Number of Residues	4
Details	binding site for residue CL E 304

Chain	Residue
E	GLY32
E	SER33
E	LYS62
E	GLY76

site_id	AD5
Number of Residues	5
Details	binding site for residue CL F 301

Chain	Residue
F	VAL30
F	ILE165
F	GLY166
F	ARG169
F	HOH403

site_id	AD6
Number of Residues	3
Details	binding site for residue CL G 301

Chain	Residue
G	HOH411
G	HOH474
N	HOH480

site_id	AD7
Number of Residues	4
Details	binding site for residue CL G 302

Chain	Residue
G	LYS186
G	GLU192
G	GLN193
G	THR197

site_id	AD8
Number of Residues	5
Details	binding site for residue K G 303

Chain	Residue
G	THR14
G	TYR125
G	ASN128
G	MET131
G	HOH450

site_id	AD9
Number of Residues	6
Details	binding site for residue MG H 301

Chain	Residue
H	GLN91
H	HOH515
N	HOH410
N	HOH411
N	HOH470
N	HOH492

site_id	AE1
Number of Residues	5
Details	binding site for residue MG H 302

Chain	Residue
H	ILE163
H	ASP166
H	SER169
H	HOH478
Z	ASP213

site_id	AE2
Number of Residues	4
Details	binding site for residue CL H 303

Chain	Residue
H	ASN80
H	ARG81
H	HOH522
N	HOH423

site_id	AE3
Number of Residues	3
Details	binding site for residue 1PE H 304

Chain	Residue
H	LEU58
H	TYR90
I	TYR95

site_id	AE4
Number of Residues	5
Details	binding site for residue MG I 301

Chain	Residue
I	VAL174
I	ASP177
I	SER180
I	HOH414
I	HOH477

site_id	AE5
Number of Residues	2
Details	binding site for residue CL I 302

Chain	Residue
I	ARG65
I	ARG69

site_id	AE6
Number of Residues	4
Details	binding site for residue 1PE I 303

Chain	Residue
I	GLU96
I	LYS97
I	HOH502
J	ASP90

site_id	AE7
Number of Residues	5
Details	binding site for residue MG Y 301

Chain	Residue
I	ASP204
Y	THR164
Y	ASP167
Y	SER170
Y	HOH442

site_id	AE8
Number of Residues	6
Details	binding site for residue MG J 301

Chain	Residue
J	HOH406
J	HOH445
J	HOH453
J	HOH495
J	HOH516
K	HOH483

site_id	AE9
Number of Residues	6
Details	binding site for residue MG K 301

Chain	Residue
K	HOH466
W	ASP204
K	THR164
K	ASP167
K	ALA168
K	SER170

site_id	AF1
Number of Residues	3
Details	binding site for residue CL K 302

Chain	Residue
K	GLY129
K	SER130
d	6VO5

site_id	AF2
Number of Residues	2
Details	binding site for residue CL K 303

Chain	Residue
K	LYS32
K	ASN175

site_id	AF3
Number of Residues	1
Details	binding site for residue CL K 304

Chain	Residue
K	ARG69

site_id	AF4
Number of Residues	2
Details	binding site for residue CL K 305

Chain	Residue
K	ILE121
K	SER122

site_id	AF5
Number of Residues	3
Details	binding site for residue 1PE K 306

Chain	Residue
K	HOH441
L	SER98
L	PHE101

site_id	AF6
Number of Residues	2
Details	binding site for residue 1PE L 301

Chain	Residue
L	PHE33
f	CL101

site_id	AF7
Number of Residues	4
Details	binding site for residue K L 302

Chain	Residue
L	ALA183
L	ASP186
L	THR189
L	HOH421

site_id	AF8
Number of Residues	5
Details	binding site for residue MG V 301

Chain	Residue
L	ASP213
V	ILE163
V	ASP166
V	SER169
V	HOH466

site_id	AF9
Number of Residues	4
Details	binding site for residue CL M 301

Chain	Residue
M	PHE36
M	ARG37
M	ASN38
N	HOH401

site_id	AG1
Number of Residues	4
Details	binding site for residue CL M 302

Chain	Residue
M	ARG35
N	PRO115
N	MET116
N	CL303

site_id	AG2
Number of Residues	1
Details	binding site for residue CL M 303

Chain	Residue
M	ARG44

site_id	AG3
Number of Residues	4
Details	binding site for residue CL N 301

Chain	Residue
N	THR31
N	ARG45
N	GLN53
e	6VO5

site_id	AG4
Number of Residues	4
Details	binding site for residue CL N 302

Chain	Residue
N	GLY47
N	GLY129
N	SER130
e	6VO5

site_id	AG5
Number of Residues	6
Details	binding site for residue CL N 303

Chain	Residue
M	ARG35
M	CL302
N	SER46
N	GLY97
N	GLY128
N	GLY129

site_id	AG6
Number of Residues	5
Details	binding site for residue CL N 304

Chain	Residue
N	ARG19
N	ARG29
N	GLY171
N	GLY172
a	HOH420

site_id	AG7
Number of Residues	5
Details	binding site for residue 1PE N 305

Chain	Residue
G	TYR103
H	PHE88
N	TYR90
N	HOH446
N	HOH503

site_id	AG8
Number of Residues	6
Details	binding site for residue K N 306

Chain	Residue
N	MET164
N	ASP167
N	SER170
N	HOH454
N	HOH490
N	HOH530

site_id	AG9
Number of Residues	3
Details	binding site for residue CL O 301

Chain	Residue
O	SER6
Q	ARG5
Q	HOH445

site_id	AH1
Number of Residues	1
Details	binding site for residue CL O 302

Chain	Residue
O	TYR100

site_id	AH2
Number of Residues	2
Details	binding site for residue CL O 303

Chain	Residue
O	ARG218
O	ARG219

site_id	AH3
Number of Residues	3
Details	binding site for residue CL O 304

Chain	Residue
O	HIS87
O	ARG90
V	SER69

site_id	AH4
Number of Residues	3
Details	binding site for residue CL P 301

Chain	Residue
P	GLU70
P	GLN95
P	HOH496

site_id	AH5
Number of Residues	1
Details	binding site for residue CL Q 301

Chain	Residue
Q	ARG95

site_id	AH6
Number of Residues	3
Details	binding site for residue CL Q 302

Chain	Residue
Q	VAL26
Q	ILE161
Q	GLY162

site_id	AH7
Number of Residues	4
Details	binding site for residue CL R 301

Chain	Residue
R	ILE170
R	GLY171
R	SER174
R	HOH491

site_id	AH8
Number of Residues	2
Details	binding site for residue CL R 302

Chain	Residue
R	THR161
S	THR78

site_id	AH9
Number of Residues	4
Details	binding site for residue CL S 301

Chain	Residue
S	6V1148
S	ILE161
S	GLY162
S	SER165

site_id	AI1
Number of Residues	2
Details	binding site for residue CL S 302

Chain	Residue
S	SER150
S	ASN152

site_id	AI2
Number of Residues	4
Details	binding site for residue CL S 303

Chain	Residue
S	GLY32
S	SER33
S	LYS62
S	GLY76

site_id	AI3
Number of Residues	1
Details	binding site for residue CL U 301

Chain	Residue
b	HOH434

site_id	AI4
Number of Residues	5
Details	binding site for residue K U 302

Chain	Residue
U	THR14
U	TYR125
U	ASN128
U	MET131
U	HOH423

site_id	AI5
Number of Residues	7
Details	binding site for residue MG V 302

Chain	Residue
V	GLN91
V	HOH446
V	HOH458
b	ASP93
b	HOH405
b	HOH445
b	HOH478

site_id	AI6
Number of Residues	4
Details	binding site for residue CL V 303

Chain	Residue
V	VAL77
V	ASN80
V	ARG81
b	HOH401

site_id	AI7
Number of Residues	5
Details	binding site for residue MG W 301

Chain	Residue
W	VAL174
W	ASP177
W	SER180
W	HOH440
W	HOH459

site_id	AI8
Number of Residues	2
Details	binding site for residue CL W 302

Chain	Residue
W	ARG65
W	ARG69

site_id	AI9
Number of Residues	3
Details	binding site for residue 1PE W 303

Chain	Residue
W	GLU96
W	LYS97
W	HOH434

site_id	AJ1
Number of Residues	6
Details	binding site for residue MG X 301

Chain	Residue
X	HOH402
X	HOH432
X	HOH441
X	HOH480
X	HOH505
Y	HOH508

site_id	AJ2
Number of Residues	3
Details	binding site for residue CL Y 302

Chain	Residue
Y	GLY129
Y	SER130
g	6VO5

site_id	AJ3
Number of Residues	2
Details	binding site for residue CL Y 303

Chain	Residue
Y	TYR66
Y	ARG69

site_id	AJ4
Number of Residues	2
Details	binding site for residue CL Y 304

Chain	Residue
Y	LYS32
Y	ASN175

site_id	AJ5
Number of Residues	2
Details	binding site for residue CL Y 305

Chain	Residue
Y	ILE121
Y	SER122

site_id	AJ6
Number of Residues	1
Details	binding site for residue CL Y 306

Chain	Residue
Y	HOH454

site_id	AJ7
Number of Residues	5
Details	binding site for residue 1PE Z 301

Chain	Residue
Y	TYR90
Z	TYR97
Z	SER98
Z	PHE101
Z	HOH461

site_id	AJ8
Number of Residues	6
Details	binding site for residue K Z 302

Chain	Residue
Z	ALA183
Z	ASP186
Z	THR189
Z	HOH434
Z	HOH456
Z	HOH464

site_id	AJ9
Number of Residues	4
Details	binding site for residue CL a 301

Chain	Residue
a	PHE36
a	ARG37
a	ASN38
b	HOH429

site_id	AK1
Number of Residues	4
Details	binding site for residue CL a 302

Chain	Residue
a	ARG35
b	PRO115
b	MET116
b	CL302

site_id	AK2
Number of Residues	2
Details	binding site for residue CL a 303

Chain	Residue
a	MET43
a	ARG44

site_id	AK3
Number of Residues	4
Details	binding site for residue 1PE a 304

Chain	Residue
H	SER129
Z	PHE33
a	TYR141
a	HOH498

site_id	AK4
Number of Residues	4
Details	binding site for residue CL b 301

Chain	Residue
b	GLY47
b	GLY129
b	SER130
h	6VO5

site_id	AK5
Number of Residues	6
Details	binding site for residue CL b 302

Chain	Residue
a	ARG35
a	CL302
b	SER46
b	GLY97
b	GLY128
b	GLY129

site_id	AK6
Number of Residues	5
Details	binding site for residue CL b 303

Chain	Residue
M	HOH434
b	ARG19
b	ARG29
b	GLY171
b	GLY172

site_id	AK7
Number of Residues	5
Details	binding site for residue CL b 304

Chain	Residue
b	THR31
b	ARG45
b	GLN53
b	HOH442
h	6VO5

site_id	AK8
Number of Residues	5
Details	binding site for residue 1PE b 305

Chain	Residue
U	TYR103
V	PHE88
V	ARG89
b	TYR61
b	HOH497

site_id	AK9
Number of Residues	5
Details	binding site for residue K b 306

Chain	Residue
b	MET164
b	ASP167
b	SER170
b	HOH410
b	HOH459

site_id	AL1
Number of Residues	4
Details	binding site for residue CL c 101

Chain	Residue
H	GLY128
H	SER129
H	HOH536
c	6VO5

site_id	AL2
Number of Residues	4
Details	binding site for residue CL f 101

Chain	Residue
L	1PE301
V	GLY128
V	SER129
f	6VO5

site_id	AL3
Number of Residues	14
Details	binding site for Ligand residues 6V1 E 148 through PRO E 149 bound to THR E 147

Chain	Residue
E	ALA25
E	PRO127
E	TYR128
E	VAL130
E	GLN146
E	THR147
E	SER150
E	ALA151
E	ASN152
E	CL301
E	CL302
E	HOH427
E	HOH438
E	HOH450

site_id	AL4
Number of Residues	11
Details	binding site for Ligand 6VO c 5 bound to THR H 1

Chain	Residue
H	THR1
H	ALA20
H	THR21
H	CYS31
H	GLY47
H	THR52
H	SER129
H	GLY168
H	HOH417
c	THR4
c	CL101

site_id	AL5
Number of Residues	11
Details	binding site for Ligand 6VO c 5 bound to THR H 1

Chain	Residue
H	THR1
H	ALA20
H	THR21
H	CYS31
H	GLY47
H	THR52
H	SER129
H	GLY168
H	HOH417
c	THR4
c	CL101

site_id	AL6
Number of Residues	8
Details	binding site for Ligand 6VO d 5 bound to THR K 1

Chain	Residue
K	THR1
K	ARG19
K	ALA20
K	GLY47
K	SER130
K	TYR169
K	CL302
d	THR4

site_id	AL7
Number of Residues	8
Details	binding site for Ligand 6VO d 5 bound to THR K 1

Chain	Residue
K	THR1
K	ARG19
K	ALA20
K	GLY47
K	SER130
K	TYR169
K	CL302
d	THR4

site_id	AL8
Number of Residues	10
Details	binding site for Ligand 6VO e 5 bound to THR N 1

Chain	Residue
N	THR1
N	ARG19
N	THR20
N	ARG45
N	GLY47
N	SER130
N	SER169
N	CL301
N	CL302
e	THR4

site_id	AL9
Number of Residues	10
Details	binding site for Ligand 6VO e 5 bound to THR N 1

Chain	Residue
N	THR1
N	ARG19
N	THR20
N	ARG45
N	GLY47
N	SER130
N	SER169
N	CL301
N	CL302
e	THR4

site_id	AM1
Number of Residues	12
Details	binding site for Ligand residues 6V1 S 148 through PRO S 149 bound to THR S 147

Chain	Residue
S	ALA25
S	PRO127
S	TYR128
S	VAL130
S	GLN146
S	THR147
S	SER150
S	ALA151
S	ASN152
S	CL301
S	HOH421
S	HOH442

site_id	AM2
Number of Residues	10
Details	binding site for Ligand 6VO f 5 bound to THR V 1

Chain	Residue
V	THR1
V	ALA20
V	THR21
V	CYS31
V	GLY47
V	SER129
V	GLY168
V	HOH430
f	THR4
f	CL101

site_id	AM3
Number of Residues	10
Details	binding site for Ligand 6VO f 5 bound to THR V 1

Chain	Residue
V	THR1
V	ALA20
V	THR21
V	CYS31
V	GLY47
V	SER129
V	GLY168
V	HOH430
f	THR4
f	CL101

site_id	AM4
Number of Residues	9
Details	binding site for Ligand 6VO g 5 bound to THR Y 1

Chain	Residue
Y	THR1
Y	ARG19
Y	ALA20
Y	GLY47
Y	SER130
Y	TYR169
Y	CL302
Y	HOH428
g	THR4

site_id	AM5
Number of Residues	9
Details	binding site for Ligand 6VO g 5 bound to THR Y 1

Chain	Residue
Y	THR1
Y	ARG19
Y	ALA20
Y	GLY47
Y	SER130
Y	TYR169
Y	CL302
Y	HOH428
g	THR4

site_id	AM6
Number of Residues	12
Details	binding site for Ligand 6VO h 5 bound to THR b 1

Chain	Residue
b	THR1
b	ARG19
b	THR20
b	THR21
b	ARG45
b	GLY47
b	THR52
b	SER130
b	SER169
b	CL301
b	CL304
h	THR4

site_id	AM7
Number of Residues	12
Details	binding site for Ligand 6VO h 5 bound to THR b 1

Chain	Residue
b	THR1
b	ARG19
b	THR20
b	THR21
b	ARG45
b	GLY47
b	THR52
b	SER130
b	SER169
b	CL301
b	CL304
h	THR4

site_id	AM8
Number of Residues	11
Details	binding site for Ligand 6VO c 5 bound to THR c 4

Chain	Residue
H	THR1
H	ALA20
H	THR21
H	CYS31
H	GLY47
H	THR52
H	SER129
H	GLY168
H	HOH417
c	THR4
c	CL101

site_id	AM9
Number of Residues	8
Details	binding site for Ligand 6VO d 5 bound to THR d 4

Chain	Residue
K	THR1
K	ARG19
K	ALA20
K	GLY47
K	SER130
K	TYR169
K	CL302
d	THR4

site_id	AN1
Number of Residues	10
Details	binding site for Ligand 6VO e 5 bound to THR e 4

Chain	Residue
N	THR1
N	ARG19
N	THR20
N	ARG45
N	GLY47
N	SER130
N	SER169
N	CL301
N	CL302
e	THR4

site_id	AN2
Number of Residues	10
Details	binding site for Ligand 6VO f 5 bound to THR f 4

Chain	Residue
V	THR1
V	ALA20
V	THR21
V	CYS31
V	GLY47
V	SER129
V	GLY168
V	HOH430
f	THR4
f	CL101

site_id	AN3
Number of Residues	9
Details	binding site for Ligand 6VO g 5 bound to THR g 4

Chain	Residue
Y	THR1
Y	ARG19
Y	ALA20
Y	GLY47
Y	SER130
Y	TYR169
Y	CL302
Y	HOH428
g	THR4

site_id	AN4
Number of Residues	12
Details	binding site for Ligand 6VO h 5 bound to THR h 4

Chain	Residue
b	THR1
b	ARG19
b	THR20
b	THR21
b	ARG45
b	GLY47
b	THR52
b	SER130
b	SER169
b	CL301
b	CL304
h	THR4

site_id	AN5
Number of Residues	12
Details	binding site for Di-peptide ASP U 46 and 6V1 U 47

Chain	Residue
U	ARG43
U	GLY44
U	LYS45
U	ALA48
U	PRO149
U	THR194
U	VAL195
U	VAL219
U	VAL220
U	THR221
U	VAL222
U	ARG228

site_id	AN6
Number of Residues	11
Details	binding site for Di-peptide 6V1 U 47 and ALA U 48

Chain	Residue
U	VAL42
U	ARG43
U	GLY44
U	ASP46
U	VAL49
U	PRO149
U	THR194
U	VAL195
U	VAL219
U	VAL220
U	ARG228

site_id	AN7
Number of Residues	12
Details	binding site for Di-peptide TYR U 160 and 6V1 U 161

Chain	Residue
O	LYS49
O	LYS50
O	TYR56
O	ASP57
O	SER60
O	VAL61
U	LYS116
U	TYR152
U	LYS153
U	TYR159
U	GLY162
U	PHE163

site_id	AN8
Number of Residues	10
Details	binding site for Di-peptide 6V1 U 161 and GLY U 162

Chain	Residue
O	LYS49
O	LYS50
O	TYR56
O	ASP57
O	SER60
U	VAL151
U	TYR152
U	LYS153
U	TYR160
U	PHE163

site_id	AN9
Number of Residues	16
Details	binding site for Di-peptide ASP X 90 and 6V1 X 91

Chain	Residue
W	ARG65
X	PHE56
X	TYR59
X	ARG86
X	ASN87
X	LEU88
X	ALA89
X	LEU92
X	ARG93
X	SER94
X	THR96
X	PRO97
X	TYR98
X	HOH425
X	HOH429
X	HOH453

site_id	AO1
Number of Residues	15
Details	binding site for Di-peptide 6V1 X 91 and LEU X 92

Chain	Residue
X	PHE56
X	TYR59
X	ASN87
X	LEU88
X	ALA89
X	ASP90
X	ARG93
X	SER94
X	THR96
X	PRO97
X	TYR98
X	LEU121
X	HOH408
X	HOH453
X	HOH458

site_id	AO2
Number of Residues	7
Details	binding site for residues ACE c 1 and IML c 2

Chain	Residue
H	GLU22
H	THR48
I	GLU105
I	ASP124
I	LEU125
c	ILE3
c	THR4

site_id	AO3
Number of Residues	11
Details	binding site for Di-peptide IML c 2 and ILE c 3

Chain	Residue
H	ALA20
H	THR21
H	GLU22
H	THR48
H	ALA49
I	ASP124
I	LEU125
I	CYS128
I	HOH406
c	ACE1
c	THR4

site_id	AO4
Number of Residues	3
Details	binding site for residues ACE d 1 and IML d 2

Chain	Residue
L	TYR107
L	PRO126
d	ILE3

site_id	AO5
Number of Residues	9
Details	binding site for Di-peptide IML d 2 and ILE d 3

Chain	Residue
K	ALA20
K	THR21
K	ALA49
L	TYR107
L	ASP125
L	PRO126
L	HOH408
d	ACE1
d	THR4

site_id	AO6
Number of Residues	3
Details	binding site for residues ACE e 1 and IML e 2

Chain	Residue
N	THR22
N	HOH466
e	ILE3

site_id	AO7
Number of Residues	9
Details	binding site for Di-peptide IML e 2 and ILE e 3

Chain	Residue
H	TYR114
N	THR20
N	THR21
N	THR22
N	ALA49
N	HOH464
N	HOH466
e	ACE1
e	THR4

site_id	AO8
Number of Residues	5
Details	binding site for residues ACE f 1 and IML f 2

Chain	Residue
W	GLU105
W	ASP124
W	LEU125
f	ILE3
f	THR4

site_id	AO9
Number of Residues	10
Details	binding site for Di-peptide IML f 2 and ILE f 3

Chain	Residue
V	ALA20
V	THR21
V	GLU22
V	ALA49
V	HOH414
W	ASP124
W	LEU125
W	CYS128
f	ACE1
f	THR4

site_id	AP1
Number of Residues	3
Details	binding site for residues ACE g 1 and IML g 2

Chain	Residue
Z	TYR107
Z	PRO126
g	ILE3

site_id	AP2
Number of Residues	9
Details	binding site for Di-peptide IML g 2 and ILE g 3

Chain	Residue
Y	ALA20
Y	THR21
Y	ALA49
Z	TYR107
Z	ASP125
Z	PRO126
Z	HOH411
g	ACE1
g	THR4

site_id	AP3
Number of Residues	2
Details	binding site for residues ACE h 1 and IML h 2

Chain	Residue
b	THR22
h	ILE3

site_id	AP4
Number of Residues	7
Details	binding site for Di-peptide IML h 2 and ILE h 3

Chain	Residue
V	SER118
b	THR21
b	THR22
b	ALA49
h	ACE1
h	THR4
h	HOH101

Functional Information from PROSITE/UniProt

site_id	PS00388
Number of Residues	23
Details	PROTEASOME_ALPHA_1 Proteasome alpha-type subunits signature. YdndvTvWSPqGRihQIEYAmeA

Chain	Residue	Details
E	TYR6-ALA28
B	TYR5-ALA27
G	PHE9-ALA31
A	TYR5-ALA27
D	TYR8-ALA30
C	TYR3-ALA25
F	TYR7-ALA29

site_id	PS00854
Number of Residues	48
Details	PROTEASOME_BETA_1 Proteasome beta-type subunits signature. LGVkFegGVVIAADmlgsygslarfrnis.RimrvnnstmlgasGdyAD

Chain	Residue	Details
a	LEU12-ASP59
H	ALA4-ASP51
B	LEU35-ASP82
L	LEU13-ASP60
b	MET4-ASP51
K	LEU4-ASP51
I	MET11-ASP58
J	ILE5-ASP52

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	2
Details	ACT_SITE: Nucleophile => ECO:0000269\|PubMed:25599644, ECO:0000269\|PubMed:27493187

Chain	Residue	Details
N	THR1
b	THR1
L	SER134
Z	SER34
Z	SER40
Z	SER134

site_id	SWS_FT_FI2
Number of Residues	2
Details	MOD_RES: Phosphothreonine => ECO:0007744\|PubMed:23186163

Chain	Residue	Details
N	THR35
b	THR35
U	LYS102
U	LYS104
T	LYS205
T	LYS229

site_id	SWS_FT_FI3
Number of Residues	2
Details	MOD_RES: N6-acetyllysine => ECO:0007744\|PubMed:19608861

Chain	Residue	Details
L	LYS176
Z	LYS176
P	LYS127
P	LYS176
O	SER13
O	SER15

site_id	SWS_FT_FI4
Number of Residues	4
Details	CARBOHYD: O-linked (GlcNAc) serine => ECO:0000250

Chain	Residue	Details
L	SER30
L	SER181
Z	SER30
Z	SER181

site_id	SWS_FT_FI5
Number of Residues	6
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)

Chain	Residue	Details
E	LYS115
R	SER63
E	LYS208
S	LYS115
S	LYS208

site_id	SWS_FT_FI6
Number of Residues	2
Details	CARBOHYD: O-linked (GlcNAc) serine => ECO:0000250

Chain	Residue	Details
D	SER198
R	SER198

site_id	SWS_FT_FI7
Number of Residues	2
Details	MOD_RES: Phosphotyrosine => ECO:0000250\|UniProtKB:P17220

Chain	Residue	Details
A	TYR120
O	TYR120

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)