Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

5LDF

Maltose binding protein genetically fused to dodecameric glutamine synthetase

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000166	molecular_function	nucleotide binding
A	0003824	molecular_function	catalytic activity
A	0004356	molecular_function	glutamine synthetase activity
A	0005524	molecular_function	ATP binding
A	0005737	cellular_component	cytoplasm
A	0006541	biological_process	glutamine metabolic process
A	0006542	biological_process	glutamine biosynthetic process
A	0016020	cellular_component	membrane
A	0016874	molecular_function	ligase activity
A	0019740	biological_process	nitrogen utilization
A	0046872	molecular_function	metal ion binding
B	0000166	molecular_function	nucleotide binding
B	0003824	molecular_function	catalytic activity
B	0004356	molecular_function	glutamine synthetase activity
B	0005524	molecular_function	ATP binding
B	0005737	cellular_component	cytoplasm
B	0006541	biological_process	glutamine metabolic process
B	0006542	biological_process	glutamine biosynthetic process
B	0016020	cellular_component	membrane
B	0016874	molecular_function	ligase activity
B	0019740	biological_process	nitrogen utilization
B	0046872	molecular_function	metal ion binding
C	0000166	molecular_function	nucleotide binding
C	0003824	molecular_function	catalytic activity
C	0004356	molecular_function	glutamine synthetase activity
C	0005524	molecular_function	ATP binding
C	0005737	cellular_component	cytoplasm
C	0006541	biological_process	glutamine metabolic process
C	0006542	biological_process	glutamine biosynthetic process
C	0016020	cellular_component	membrane
C	0016874	molecular_function	ligase activity
C	0019740	biological_process	nitrogen utilization
C	0046872	molecular_function	metal ion binding
D	0000166	molecular_function	nucleotide binding
D	0003824	molecular_function	catalytic activity
D	0004356	molecular_function	glutamine synthetase activity
D	0005524	molecular_function	ATP binding
D	0005737	cellular_component	cytoplasm
D	0006541	biological_process	glutamine metabolic process
D	0006542	biological_process	glutamine biosynthetic process
D	0016020	cellular_component	membrane
D	0016874	molecular_function	ligase activity
D	0019740	biological_process	nitrogen utilization
D	0046872	molecular_function	metal ion binding
E	0000166	molecular_function	nucleotide binding
E	0003824	molecular_function	catalytic activity
E	0004356	molecular_function	glutamine synthetase activity
E	0005524	molecular_function	ATP binding
E	0005737	cellular_component	cytoplasm
E	0006541	biological_process	glutamine metabolic process
E	0006542	biological_process	glutamine biosynthetic process
E	0016020	cellular_component	membrane
E	0016874	molecular_function	ligase activity
E	0019740	biological_process	nitrogen utilization
E	0046872	molecular_function	metal ion binding
F	0000166	molecular_function	nucleotide binding
F	0003824	molecular_function	catalytic activity
F	0004356	molecular_function	glutamine synthetase activity
F	0005524	molecular_function	ATP binding
F	0005737	cellular_component	cytoplasm
F	0006541	biological_process	glutamine metabolic process
F	0006542	biological_process	glutamine biosynthetic process
F	0016020	cellular_component	membrane
F	0016874	molecular_function	ligase activity
F	0019740	biological_process	nitrogen utilization
F	0046872	molecular_function	metal ion binding
G	0000166	molecular_function	nucleotide binding
G	0003824	molecular_function	catalytic activity
G	0004356	molecular_function	glutamine synthetase activity
G	0005524	molecular_function	ATP binding
G	0005737	cellular_component	cytoplasm
G	0006541	biological_process	glutamine metabolic process
G	0006542	biological_process	glutamine biosynthetic process
G	0016020	cellular_component	membrane
G	0016874	molecular_function	ligase activity
G	0019740	biological_process	nitrogen utilization
G	0046872	molecular_function	metal ion binding
H	0000166	molecular_function	nucleotide binding
H	0003824	molecular_function	catalytic activity
H	0004356	molecular_function	glutamine synthetase activity
H	0005524	molecular_function	ATP binding
H	0005737	cellular_component	cytoplasm
H	0006541	biological_process	glutamine metabolic process
H	0006542	biological_process	glutamine biosynthetic process
H	0016020	cellular_component	membrane
H	0016874	molecular_function	ligase activity
H	0019740	biological_process	nitrogen utilization
H	0046872	molecular_function	metal ion binding
I	0000166	molecular_function	nucleotide binding
I	0003824	molecular_function	catalytic activity
I	0004356	molecular_function	glutamine synthetase activity
I	0005524	molecular_function	ATP binding
I	0005737	cellular_component	cytoplasm
I	0006541	biological_process	glutamine metabolic process
I	0006542	biological_process	glutamine biosynthetic process
I	0016020	cellular_component	membrane
I	0016874	molecular_function	ligase activity
I	0019740	biological_process	nitrogen utilization
I	0046872	molecular_function	metal ion binding
J	0000166	molecular_function	nucleotide binding
J	0003824	molecular_function	catalytic activity
J	0004356	molecular_function	glutamine synthetase activity
J	0005524	molecular_function	ATP binding
J	0005737	cellular_component	cytoplasm
J	0006541	biological_process	glutamine metabolic process
J	0006542	biological_process	glutamine biosynthetic process
J	0016020	cellular_component	membrane
J	0016874	molecular_function	ligase activity
J	0019740	biological_process	nitrogen utilization
J	0046872	molecular_function	metal ion binding
K	0000166	molecular_function	nucleotide binding
K	0003824	molecular_function	catalytic activity
K	0004356	molecular_function	glutamine synthetase activity
K	0005524	molecular_function	ATP binding
K	0005737	cellular_component	cytoplasm
K	0006541	biological_process	glutamine metabolic process
K	0006542	biological_process	glutamine biosynthetic process
K	0016020	cellular_component	membrane
K	0016874	molecular_function	ligase activity
K	0019740	biological_process	nitrogen utilization
K	0046872	molecular_function	metal ion binding
L	0000166	molecular_function	nucleotide binding
L	0003824	molecular_function	catalytic activity
L	0004356	molecular_function	glutamine synthetase activity
L	0005524	molecular_function	ATP binding
L	0005737	cellular_component	cytoplasm
L	0006541	biological_process	glutamine metabolic process
L	0006542	biological_process	glutamine biosynthetic process
L	0016020	cellular_component	membrane
L	0016874	molecular_function	ligase activity
L	0019740	biological_process	nitrogen utilization
L	0046872	molecular_function	metal ion binding
M	0008643	biological_process	carbohydrate transport
M	0015144	molecular_function	carbohydrate transmembrane transporter activity
M	0015768	biological_process	maltose transport
M	0030288	cellular_component	outer membrane-bounded periplasmic space
M	0034219	biological_process	carbohydrate transmembrane transport
M	0042597	cellular_component	periplasmic space
M	0042956	biological_process	maltodextrin transmembrane transport
M	0055052	cellular_component	ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing
M	0055085	biological_process	transmembrane transport
M	1901982	molecular_function	maltose binding
N	0008643	biological_process	carbohydrate transport
N	0015144	molecular_function	carbohydrate transmembrane transporter activity
N	0015768	biological_process	maltose transport
N	0030288	cellular_component	outer membrane-bounded periplasmic space
N	0034219	biological_process	carbohydrate transmembrane transport
N	0042597	cellular_component	periplasmic space
N	0042956	biological_process	maltodextrin transmembrane transport
N	0055052	cellular_component	ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing
N	0055085	biological_process	transmembrane transport
N	1901982	molecular_function	maltose binding
O	0008643	biological_process	carbohydrate transport
O	0015144	molecular_function	carbohydrate transmembrane transporter activity
O	0015768	biological_process	maltose transport
O	0030288	cellular_component	outer membrane-bounded periplasmic space
O	0034219	biological_process	carbohydrate transmembrane transport
O	0042597	cellular_component	periplasmic space
O	0042956	biological_process	maltodextrin transmembrane transport
O	0055052	cellular_component	ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing
O	0055085	biological_process	transmembrane transport
O	1901982	molecular_function	maltose binding
P	0008643	biological_process	carbohydrate transport
P	0015144	molecular_function	carbohydrate transmembrane transporter activity
P	0015768	biological_process	maltose transport
P	0030288	cellular_component	outer membrane-bounded periplasmic space
P	0034219	biological_process	carbohydrate transmembrane transport
P	0042597	cellular_component	periplasmic space
P	0042956	biological_process	maltodextrin transmembrane transport
P	0055052	cellular_component	ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing
P	0055085	biological_process	transmembrane transport
P	1901982	molecular_function	maltose binding
Q	0008643	biological_process	carbohydrate transport
Q	0015144	molecular_function	carbohydrate transmembrane transporter activity
Q	0015768	biological_process	maltose transport
Q	0030288	cellular_component	outer membrane-bounded periplasmic space
Q	0034219	biological_process	carbohydrate transmembrane transport
Q	0042597	cellular_component	periplasmic space
Q	0042956	biological_process	maltodextrin transmembrane transport
Q	0055052	cellular_component	ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing
Q	0055085	biological_process	transmembrane transport
Q	1901982	molecular_function	maltose binding
R	0008643	biological_process	carbohydrate transport
R	0015144	molecular_function	carbohydrate transmembrane transporter activity
R	0015768	biological_process	maltose transport
R	0030288	cellular_component	outer membrane-bounded periplasmic space
R	0034219	biological_process	carbohydrate transmembrane transport
R	0042597	cellular_component	periplasmic space
R	0042956	biological_process	maltodextrin transmembrane transport
R	0055052	cellular_component	ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing
R	0055085	biological_process	transmembrane transport
R	1901982	molecular_function	maltose binding
S	0008643	biological_process	carbohydrate transport
S	0015144	molecular_function	carbohydrate transmembrane transporter activity
S	0015768	biological_process	maltose transport
S	0030288	cellular_component	outer membrane-bounded periplasmic space
S	0034219	biological_process	carbohydrate transmembrane transport
S	0042597	cellular_component	periplasmic space
S	0042956	biological_process	maltodextrin transmembrane transport
S	0055052	cellular_component	ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing
S	0055085	biological_process	transmembrane transport
S	1901982	molecular_function	maltose binding
T	0008643	biological_process	carbohydrate transport
T	0015144	molecular_function	carbohydrate transmembrane transporter activity
T	0015768	biological_process	maltose transport
T	0030288	cellular_component	outer membrane-bounded periplasmic space
T	0034219	biological_process	carbohydrate transmembrane transport
T	0042597	cellular_component	periplasmic space
T	0042956	biological_process	maltodextrin transmembrane transport
T	0055052	cellular_component	ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing
T	0055085	biological_process	transmembrane transport
T	1901982	molecular_function	maltose binding
U	0008643	biological_process	carbohydrate transport
U	0015144	molecular_function	carbohydrate transmembrane transporter activity
U	0015768	biological_process	maltose transport
U	0030288	cellular_component	outer membrane-bounded periplasmic space
U	0034219	biological_process	carbohydrate transmembrane transport
U	0042597	cellular_component	periplasmic space
U	0042956	biological_process	maltodextrin transmembrane transport
U	0055052	cellular_component	ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing
U	0055085	biological_process	transmembrane transport
U	1901982	molecular_function	maltose binding
V	0008643	biological_process	carbohydrate transport
V	0015144	molecular_function	carbohydrate transmembrane transporter activity
V	0015768	biological_process	maltose transport
V	0030288	cellular_component	outer membrane-bounded periplasmic space
V	0034219	biological_process	carbohydrate transmembrane transport
V	0042597	cellular_component	periplasmic space
V	0042956	biological_process	maltodextrin transmembrane transport
V	0055052	cellular_component	ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing
V	0055085	biological_process	transmembrane transport
V	1901982	molecular_function	maltose binding
W	0008643	biological_process	carbohydrate transport
W	0015144	molecular_function	carbohydrate transmembrane transporter activity
W	0015768	biological_process	maltose transport
W	0030288	cellular_component	outer membrane-bounded periplasmic space
W	0034219	biological_process	carbohydrate transmembrane transport
W	0042597	cellular_component	periplasmic space
W	0042956	biological_process	maltodextrin transmembrane transport
W	0055052	cellular_component	ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing
W	0055085	biological_process	transmembrane transport
W	1901982	molecular_function	maltose binding
X	0008643	biological_process	carbohydrate transport
X	0015144	molecular_function	carbohydrate transmembrane transporter activity
X	0015768	biological_process	maltose transport
X	0030288	cellular_component	outer membrane-bounded periplasmic space
X	0034219	biological_process	carbohydrate transmembrane transport
X	0042597	cellular_component	periplasmic space
X	0042956	biological_process	maltodextrin transmembrane transport
X	0055052	cellular_component	ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing
X	0055085	biological_process	transmembrane transport
X	1901982	molecular_function	maltose binding

Functional Information from PROSITE/UniProt

site_id	PS00180
Number of Residues	19
Details	GLNA_1 Glutamine synthetase signature 1. FDGSSiggwkginESDmvL

Chain	Residue	Details
A	PHE49-LEU67

site_id	PS00181
Number of Residues	16
Details	GLNA_ATP Glutamine synthetase putative ATP-binding region signature. KPMfgd..NGSGmHchmS

Chain	Residue	Details
A	LYS258-SER273

site_id	PS00182
Number of Residues	13
Details	GLNA_ADENYLATION Glutamine synthetase class-I adenylation site. KIhpgepMDKNLY

Chain	Residue	Details
A	LYS385-TYR397

site_id	PS01037
Number of Residues	18
Details	SBP_BACTERIAL_1 Bacterial extracellular solute-binding proteins, family 1 signature. PIAvEalSLIYNkdlLpN

Chain	Residue	Details
M	PRO107-ASN124

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	156
Details	BINDING: BINDING => ECO:0000250\|UniProtKB:P0A1P6

Chain	Residue	Details
A	GLU129
A	ARG321
H	SER273
H	ARG321
H	GLU327
H	GLU357
H	ARG359
I	GLU129
I	GLU131
I	GLU207
I	GLU212
I	GLU220
A	GLU327
I	ASN264
I	HIS269
I	HIS271
I	SER273
I	ARG321
I	GLU327
I	GLU357
I	ARG359
J	GLU129
J	GLU131
A	GLU357
J	GLU207
J	GLU212
J	GLU220
J	ASN264
J	HIS269
J	HIS271
J	SER273
J	ARG321
J	GLU327
J	GLU357
A	ARG359
J	ARG359
K	GLU129
K	GLU131
K	GLU207
K	GLU212
K	GLU220
K	ASN264
K	HIS269
K	HIS271
K	SER273
B	GLU129
K	ARG321
K	GLU327
K	GLU357
K	ARG359
L	GLU129
L	GLU131
L	GLU207
L	GLU212
L	GLU220
L	ASN264
B	GLU131
L	HIS269
L	HIS271
L	SER273
L	ARG321
L	GLU327
L	GLU357
L	ARG359
B	GLU207
B	GLU212
B	GLU220
B	ASN264
A	GLU131
B	HIS269
B	HIS271
B	SER273
B	ARG321
B	GLU327
B	GLU357
B	ARG359
C	GLU129
C	GLU131
C	GLU207
A	GLU207
C	GLU212
C	GLU220
C	ASN264
C	HIS269
C	HIS271
C	SER273
C	ARG321
C	GLU327
C	GLU357
C	ARG359
A	GLU212
D	GLU129
D	GLU131
D	GLU207
D	GLU212
D	GLU220
D	ASN264
D	HIS269
D	HIS271
D	SER273
D	ARG321
A	GLU220
D	GLU327
D	GLU357
D	ARG359
E	GLU129
E	GLU131
E	GLU207
E	GLU212
E	GLU220
E	ASN264
E	HIS269
A	ASN264
E	HIS271
E	SER273
E	ARG321
E	GLU327
E	GLU357
E	ARG359
F	GLU129
F	GLU131
F	GLU207
F	GLU212
A	HIS269
F	GLU220
F	ASN264
F	HIS269
F	HIS271
F	SER273
F	ARG321
F	GLU327
F	GLU357
F	ARG359
G	GLU129
A	HIS271
G	GLU131
G	GLU207
G	GLU212
G	GLU220
G	ASN264
G	HIS269
G	HIS271
G	SER273
G	ARG321
G	GLU327
A	SER273
G	GLU357
G	ARG359
H	GLU129
H	GLU131
H	GLU207
H	GLU212
H	GLU220
H	ASN264
H	HIS269
H	HIS271

site_id	SWS_FT_FI2
Number of Residues	12
Details	BINDING: BINDING => ECO:0000250\|UniProtKB:P12425

Chain	Residue	Details
A	GLY265
J	GLY265
K	GLY265
L	GLY265
B	GLY265
C	GLY265
D	GLY265
E	GLY265
F	GLY265
G	GLY265
H	GLY265
I	GLY265

site_id	SWS_FT_FI3
Number of Residues	24
Details	BINDING: BINDING => ECO:0000250\|UniProtKB:P9WN39

Chain	Residue	Details
A	ARG339
E	ARG344
F	ARG339
F	ARG344
G	ARG339
G	ARG344
H	ARG339
H	ARG344
I	ARG339
I	ARG344
J	ARG339
A	ARG344
J	ARG344
K	ARG339
K	ARG344
L	ARG339
L	ARG344
B	ARG339
B	ARG344
C	ARG339
C	ARG344
D	ARG339
D	ARG344
E	ARG339

site_id	SWS_FT_FI4
Number of Residues	12
Details	BINDING: BINDING => ECO:0000250\|UniProtKB:P77961

Chain	Residue	Details
A	LYS352
J	LYS352
K	LYS352
L	LYS352
B	LYS352
C	LYS352
D	LYS352
E	LYS352
F	LYS352
G	LYS352
H	LYS352
I	LYS352

site_id	SWS_FT_FI5
Number of Residues	12
Details	MOD_RES: O-AMP-tyrosine => ECO:0000250\|UniProtKB:P9WN39

Chain	Residue	Details
A	TYR397
J	TYR397
K	TYR397
L	TYR397
B	TYR397
C	TYR397
D	TYR397
E	TYR397
F	TYR397
G	TYR397
H	TYR397
I	TYR397

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)