5LDF
Maltose binding protein genetically fused to dodecameric glutamine synthetase
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | FEI TECNAI F30 |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 6.2 |
| Number of Particle | 39167 |
| Software | EMAN,IMAGIC,RELION,CTFFIND,Bsoft,UCSF Chimera |
| Symmetry | D6 |
| CTF correction | PHASE FLIPPING ONLY |
