Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

5LC5

Structure of mammalian respiratory Complex I, class2

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0005739	cellular_component	mitochondrion
A	0005743	cellular_component	mitochondrial inner membrane
A	0006120	biological_process	mitochondrial electron transport, NADH to ubiquinone
A	0008137	molecular_function	NADH dehydrogenase (ubiquinone) activity
A	0045271	cellular_component	respiratory chain complex I
A	1902600	biological_process	proton transmembrane transport
B	0008137	molecular_function	NADH dehydrogenase (ubiquinone) activity
B	0048038	molecular_function	quinone binding
B	0051536	molecular_function	iron-sulfur cluster binding
B	0051539	molecular_function	4 iron, 4 sulfur cluster binding
C	0008137	molecular_function	NADH dehydrogenase (ubiquinone) activity
C	0016651	molecular_function	oxidoreductase activity, acting on NAD(P)H
D	0003954	molecular_function	NADH dehydrogenase activity
D	0005654	cellular_component	nucleoplasm
D	0005739	cellular_component	mitochondrion
D	0005743	cellular_component	mitochondrial inner membrane
D	0006120	biological_process	mitochondrial electron transport, NADH to ubiquinone
D	0008137	molecular_function	NADH dehydrogenase (ubiquinone) activity
D	0016491	molecular_function	oxidoreductase activity
D	0016651	molecular_function	oxidoreductase activity, acting on NAD(P)H
D	0019826	molecular_function	oxygen sensor activity
D	0022008	biological_process	neurogenesis
D	0031625	molecular_function	ubiquitin protein ligase binding
D	0032981	biological_process	mitochondrial respiratory chain complex I assembly
D	0042063	biological_process	gliogenesis
D	0042775	biological_process	mitochondrial ATP synthesis coupled electron transport
D	0045271	cellular_component	respiratory chain complex I
D	0046872	molecular_function	metal ion binding
D	0048038	molecular_function	quinone binding
D	0051287	molecular_function	NAD binding
D	0051539	molecular_function	4 iron, 4 sulfur cluster binding
D	0061351	biological_process	neural precursor cell proliferation
D	0071453	biological_process	cellular response to oxygen levels
D	1902600	biological_process	proton transmembrane transport
E	0003954	molecular_function	NADH dehydrogenase activity
E	0005739	cellular_component	mitochondrion
E	0005743	cellular_component	mitochondrial inner membrane
E	0006120	biological_process	mitochondrial electron transport, NADH to ubiquinone
E	0008137	molecular_function	NADH dehydrogenase (ubiquinone) activity
E	0016491	molecular_function	oxidoreductase activity
E	0045271	cellular_component	respiratory chain complex I
E	0046872	molecular_function	metal ion binding
E	0051537	molecular_function	2 iron, 2 sulfur cluster binding
E	1902600	biological_process	proton transmembrane transport
F	0005739	cellular_component	mitochondrion
F	0005743	cellular_component	mitochondrial inner membrane
F	0006120	biological_process	mitochondrial electron transport, NADH to ubiquinone
F	0008137	molecular_function	NADH dehydrogenase (ubiquinone) activity
F	0010181	molecular_function	FMN binding
F	0016491	molecular_function	oxidoreductase activity
F	0045271	cellular_component	respiratory chain complex I
F	0046872	molecular_function	metal ion binding
F	0051287	molecular_function	NAD binding
F	0051539	molecular_function	4 iron, 4 sulfur cluster binding
F	1902600	biological_process	proton transmembrane transport
G	0008137	molecular_function	NADH dehydrogenase (ubiquinone) activity
G	0016020	cellular_component	membrane
G	0016491	molecular_function	oxidoreductase activity
G	0042773	biological_process	ATP synthesis coupled electron transport
G	0051536	molecular_function	iron-sulfur cluster binding
H	0003954	molecular_function	NADH dehydrogenase activity
H	0005739	cellular_component	mitochondrion
H	0005743	cellular_component	mitochondrial inner membrane
H	0008137	molecular_function	NADH dehydrogenase (ubiquinone) activity
H	0009060	biological_process	aerobic respiration
H	0016020	cellular_component	membrane
H	0032981	biological_process	mitochondrial respiratory chain complex I assembly
H	0045271	cellular_component	respiratory chain complex I
H	1902600	biological_process	proton transmembrane transport
I	0016020	cellular_component	membrane
I	0016651	molecular_function	oxidoreductase activity, acting on NAD(P)H
I	0051539	molecular_function	4 iron, 4 sulfur cluster binding
J	0005739	cellular_component	mitochondrion
J	0005743	cellular_component	mitochondrial inner membrane
J	0006120	biological_process	mitochondrial electron transport, NADH to ubiquinone
J	0008137	molecular_function	NADH dehydrogenase (ubiquinone) activity
J	0032981	biological_process	mitochondrial respiratory chain complex I assembly
J	1902600	biological_process	proton transmembrane transport
K	0005739	cellular_component	mitochondrion
K	0005743	cellular_component	mitochondrial inner membrane
K	0008137	molecular_function	NADH dehydrogenase (ubiquinone) activity
K	0016651	molecular_function	oxidoreductase activity, acting on NAD(P)H
K	0042773	biological_process	ATP synthesis coupled electron transport
K	0045271	cellular_component	respiratory chain complex I
K	1902600	biological_process	proton transmembrane transport
L	0003954	molecular_function	NADH dehydrogenase activity
L	0005739	cellular_component	mitochondrion
L	0005743	cellular_component	mitochondrial inner membrane
L	0006120	biological_process	mitochondrial electron transport, NADH to ubiquinone
L	0008137	molecular_function	NADH dehydrogenase (ubiquinone) activity
L	0015990	biological_process	electron transport coupled proton transport
L	0032981	biological_process	mitochondrial respiratory chain complex I assembly
L	0042773	biological_process	ATP synthesis coupled electron transport
L	0045271	cellular_component	respiratory chain complex I
M	0003954	molecular_function	NADH dehydrogenase activity
M	0005739	cellular_component	mitochondrion
M	0005743	cellular_component	mitochondrial inner membrane
M	0006120	biological_process	mitochondrial electron transport, NADH to ubiquinone
M	0008137	molecular_function	NADH dehydrogenase (ubiquinone) activity
M	0009060	biological_process	aerobic respiration
M	0015990	biological_process	electron transport coupled proton transport
M	0032981	biological_process	mitochondrial respiratory chain complex I assembly
M	0042773	biological_process	ATP synthesis coupled electron transport
M	0045271	cellular_component	respiratory chain complex I
M	0048039	molecular_function	ubiquinone binding
N	0005739	cellular_component	mitochondrion
N	0005743	cellular_component	mitochondrial inner membrane
N	0006120	biological_process	mitochondrial electron transport, NADH to ubiquinone
N	0008137	molecular_function	NADH dehydrogenase (ubiquinone) activity
N	0032981	biological_process	mitochondrial respiratory chain complex I assembly
N	0045271	cellular_component	respiratory chain complex I
N	1902600	biological_process	proton transmembrane transport
T	0006633	biological_process	fatty acid biosynthetic process
U	0006633	biological_process	fatty acid biosynthetic process
V	0005739	cellular_component	mitochondrion
V	0005743	cellular_component	mitochondrial inner membrane
V	0022904	biological_process	respiratory electron transport chain
V	0032981	biological_process	mitochondrial respiratory chain complex I assembly
V	0045271	cellular_component	respiratory chain complex I
W	0005739	cellular_component	mitochondrion
W	0005743	cellular_component	mitochondrial inner membrane
W	0006979	biological_process	response to oxidative stress
W	0032981	biological_process	mitochondrial respiratory chain complex I assembly
W	0045271	cellular_component	respiratory chain complex I
X	0006120	biological_process	mitochondrial electron transport, NADH to ubiquinone
Y	0005739	cellular_component	mitochondrion
Y	0005743	cellular_component	mitochondrial inner membrane
Y	0032981	biological_process	mitochondrial respiratory chain complex I assembly
Y	0045271	cellular_component	respiratory chain complex I
a	0005739	cellular_component	mitochondrion
a	0005743	cellular_component	mitochondrial inner membrane
a	0032981	biological_process	mitochondrial respiratory chain complex I assembly
a	0045271	cellular_component	respiratory chain complex I
b	0005743	cellular_component	mitochondrial inner membrane
b	0045271	cellular_component	respiratory chain complex I
c	0005739	cellular_component	mitochondrion
c	0005743	cellular_component	mitochondrial inner membrane
c	0032981	biological_process	mitochondrial respiratory chain complex I assembly
c	0045271	cellular_component	respiratory chain complex I
d	0005743	cellular_component	mitochondrial inner membrane
d	0006120	biological_process	mitochondrial electron transport, NADH to ubiquinone
e	0005739	cellular_component	mitochondrion
e	0005743	cellular_component	mitochondrial inner membrane
e	0005758	cellular_component	mitochondrial intermembrane space
e	0032981	biological_process	mitochondrial respiratory chain complex I assembly
e	0045271	cellular_component	respiratory chain complex I
f	0005739	cellular_component	mitochondrion
f	0005743	cellular_component	mitochondrial inner membrane
f	0032981	biological_process	mitochondrial respiratory chain complex I assembly
f	0045271	cellular_component	respiratory chain complex I
g	0005739	cellular_component	mitochondrion
g	0005743	cellular_component	mitochondrial inner membrane
g	0032981	biological_process	mitochondrial respiratory chain complex I assembly
g	0045271	cellular_component	respiratory chain complex I
i	0006120	biological_process	mitochondrial electron transport, NADH to ubiquinone
m	0005739	cellular_component	mitochondrion
n	0006120	biological_process	mitochondrial electron transport, NADH to ubiquinone
o	0005739	cellular_component	mitochondrion
q	0005739	cellular_component	mitochondrion
q	0005743	cellular_component	mitochondrial inner membrane
q	0006979	biological_process	response to oxidative stress
q	0016020	cellular_component	membrane
q	0032981	biological_process	mitochondrial respiratory chain complex I assembly
q	0045271	cellular_component	respiratory chain complex I

Functional Information from PDB Data

site_id	AC1
Number of Residues	8
Details	binding site for residue SF4 B 201

Chain	Residue
B	ALA53
B	CYS54
B	CYS55
B	GLY90
B	SER118
B	CYS119
B	CYS149
B	PRO150

site_id	AC2
Number of Residues	5
Details	binding site for residue FES E 301

Chain	Residue
E	CYS108
E	CYS144
E	ALA147
E	CYS148
E	CYS103

site_id	AC3
Number of Residues	15
Details	binding site for residue FMN F 501

Chain	Residue
F	ARG68
F	GLY69
F	GLY70
F	ALA71
F	ASN96
F	ASP98
F	GLU99
F	GLY100
F	CYS186
F	GLY187
F	VAL222
F	ALA223
F	ASN224
F	THR227
F	ALA406

site_id	AC4
Number of Residues	6
Details	binding site for residue SF4 F 502

Chain	Residue
F	PRO203
F	CYS359
F	GLN361
F	CYS362
F	CYS365
F	CYS405

site_id	AC5
Number of Residues	8
Details	binding site for residue SF4 G 801

Chain	Residue
G	HIS101
G	ASP104
G	CYS105
G	CYS108
G	GLN110
G	CYS114
G	GLN117
G	GLY206

site_id	AC6
Number of Residues	5
Details	binding site for residue SF4 G 802

Chain	Residue
G	CYS153
G	ILE154
G	CYS156
G	CYS159
G	CYS203

site_id	AC7
Number of Residues	7
Details	binding site for residue FES G 803

Chain	Residue
G	PHE40
G	CYS41
G	ALA49
G	GLY50
G	CYS52
G	CYS55
G	CYS69

site_id	AC8
Number of Residues	7
Details	binding site for residue SF4 I 201

Chain	Residue
I	HIS65
I	CYS87
I	CYS116
I	ILE117
I	TYR118
I	CYS119
I	CYS122

site_id	AC9
Number of Residues	7
Details	binding site for residue SF4 I 202

Chain	Residue
I	CYS77
I	ILE78
I	ALA79
I	CYS80
I	CYS83
I	TYR109
I	CYS126

site_id	AD1
Number of Residues	4
Details	binding site for residue ZN R 201

Chain	Residue
R	CYS59
R	HIS68
R	CYS84
R	CYS87

Functional Information from PROSITE/UniProt

site_id	PS00012
Number of Residues	16
Details	PHOSPHOPANTETHEINE Phosphopantetheine attachment site. DLGLDSLDQVEIIMAM

Chain	Residue	Details
U	ASP39-MET54
T	ASP39-MET54

site_id	PS00018
Number of Residues	13
Details	EF_HAND_1 EF-hand calcium-binding domain. DIDAEKLMCpqEI

Chain	Residue	Details
U	ASP64-ILE76
T	ASP64-ILE76

site_id	PS00198
Number of Residues	12
Details	4FE4S_FER_1 4Fe-4S ferredoxin-type iron-sulfur binding region signature. CiACKlCEaVCP

Chain	Residue	Details
I	CYS77-PRO88
I	CYS116-PRO127

site_id	PS00535
Number of Residues	12
Details	COMPLEX1_49K Respiratory chain NADH dehydrogenase 49 Kd subunit signature. LHRGtEKLiEyK

Chain	Residue	Details
D	LEU83-LYS94

site_id	PS00542
Number of Residues	22
Details	COMPLEX1_30K Respiratory chain NADH dehydrogenase 30 Kd subunit signature. EREiwDMFgvffanHpdlRrIL

Chain	Residue	Details
C	GLU131-LEU152

site_id	PS00641
Number of Residues	18
Details	COMPLEX1_75K_1 Respiratory-chain NADH dehydrogenase 75 Kd subunit signature 1. PrfCYherlsvaGnCRmC

Chain	Residue	Details
G	PRO38-CYS55

site_id	PS00642
Number of Residues	13
Details	COMPLEX1_75K_2 Respiratory-chain NADH dehydrogenase 75 Kd subunit signature 2. CPiCDqGGeCdLQ

Chain	Residue	Details
G	CYS105-GLN117

site_id	PS00643
Number of Residues	11
Details	COMPLEX1_75K_3 Respiratory-chain NADH dehydrogenase 75 Kd subunit signature 3. RCIqCtRCIrF

Chain	Residue	Details
G	ARG152-PHE162

site_id	PS00644
Number of Residues	16
Details	COMPLEX1_51K_1 Respiratory-chain NADH dehydrogenase 51 Kd subunit signature 1. GAGAYICGEETALIES

Chain	Residue	Details
F	GLY180-SER195

site_id	PS00645
Number of Residues	12
Details	COMPLEX1_51K_2 Respiratory-chain NADH dehydrogenase 51 Kd subunit signature 2. ESCGqCtPCReG

Chain	Residue	Details
F	GLU357-GLY368

site_id	PS00667
Number of Residues	16
Details	COMPLEX1_ND1_1 Respiratory-chain NADH dehydrogenase subunit 1 signature 1. GLLQpIaDAIKLFiKE

Chain	Residue	Details
H	GLY44-GLU59

site_id	PS00668
Number of Residues	14
Details	COMPLEX1_ND1_2 Respiratory-chain NADH dehydrogenase subunit 1 signature 2. PFDLTEGEseLVs.G

Chain	Residue	Details
H	PRO197-GLY210

site_id	PS01099
Number of Residues	19
Details	COMPLEX1_24K Respiratory-chain NADH dehydrogenase 24 Kd subunit signature. DklFTlieveCLGaCvnAP

Chain	Residue	Details
E	ASP134-PRO152

site_id	PS01150
Number of Residues	17
Details	COMPLEX1_20K Respiratory-chain NADH dehydrogenase 20 Kd subunit signature. GcDRIVPVDIYvPgCPP

Chain	Residue	Details
B	GLY135-PRO151

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	16
Details	TRANSMEM: Helical => ECO:0000255

Chain	Residue	Details
f	HIS10-LEU26
N	ASN274-MET294
N	PHE325-VAL345
M	ILE393-MET413
M	ALA435-LEU455
L	MET482-LEU502
L	GLY582-PHE602
Y	ARG57-VAL79
N	SER67-MET87
N	MET96-PRO116
N	ILE122-LEU142
N	ILE145-GLY165
N	ILE178-PRO198
N	MET200-MET220
N	ILE239-GLY259

site_id	SWS_FT_FI2
Number of Residues	1
Details	MOD_RES: N-acetylalanine => ECO:0000269\|PubMed:7958365

Chain	Residue	Details
b	ALA1
V	LYS59
G	CYS108
G	CYS114

site_id	SWS_FT_FI3
Number of Residues	1
Details	MOD_RES: N6-acetyllysine => ECO:0000250\|UniProtKB:Q9CPP6

Chain	Residue	Details
V	LYS45

site_id	SWS_FT_FI4
Number of Residues	1
Details	MOD_RES: Phosphoserine => ECO:0000250\|UniProtKB:Q63362

Chain	Residue	Details
V	SER88
F	LYS355

site_id	SWS_FT_FI5
Number of Residues	1
Details	MOD_RES: N6-succinyllysine; alternate => ECO:0000250\|UniProtKB:Q9CPP6

Chain	Residue	Details
V	LYS97

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)