5L95
Crystal structure of human UBA5 in complex with UFM1 and AMP
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0008641 | molecular_function | ubiquitin-like modifier activating enzyme activity |
| B | 0008641 | molecular_function | ubiquitin-like modifier activating enzyme activity |
| C | 0005515 | molecular_function | protein binding |
| C | 0005634 | cellular_component | nucleus |
| C | 0005737 | cellular_component | cytoplasm |
| C | 0005783 | cellular_component | endoplasmic reticulum |
| C | 0007420 | biological_process | brain development |
| C | 0033146 | biological_process | regulation of intracellular estrogen receptor signaling pathway |
| C | 0034976 | biological_process | response to endoplasmic reticulum stress |
| C | 0042308 | biological_process | negative regulation of protein import into nucleus |
| C | 0043066 | biological_process | negative regulation of apoptotic process |
| C | 0061709 | biological_process | reticulophagy |
| C | 0071569 | biological_process | protein ufmylation |
| C | 1990592 | biological_process | protein K69-linked ufmylation |
| D | 0005515 | molecular_function | protein binding |
| D | 0005634 | cellular_component | nucleus |
| D | 0005737 | cellular_component | cytoplasm |
| D | 0005783 | cellular_component | endoplasmic reticulum |
| D | 0007420 | biological_process | brain development |
| D | 0033146 | biological_process | regulation of intracellular estrogen receptor signaling pathway |
| D | 0034976 | biological_process | response to endoplasmic reticulum stress |
| D | 0042308 | biological_process | negative regulation of protein import into nucleus |
| D | 0043066 | biological_process | negative regulation of apoptotic process |
| D | 0061709 | biological_process | reticulophagy |
| D | 0071569 | biological_process | protein ufmylation |
| D | 1990592 | biological_process | protein K69-linked ufmylation |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 21 |
| Details | binding site for residue AMP A 401 |
| Chain | Residue |
| A | GLY80 |
| A | ILE151 |
| A | THR152 |
| A | CYS181 |
| A | VAL182 |
| A | ASP183 |
| A | ASN184 |
| A | ALA187 |
| A | HOH529 |
| A | HOH534 |
| A | HOH566 |
| A | GLY82 |
| C | VAL82 |
| C | GLY83 |
| A | GLY83 |
| A | ASP104 |
| A | TYR105 |
| A | ASP106 |
| A | LYS127 |
| A | TYR149 |
| A | ASN150 |
| site_id | AC2 |
| Number of Residues | 4 |
| Details | binding site for residue ZN A 402 |
| Chain | Residue |
| A | CYS226 |
| A | CYS229 |
| A | CYS303 |
| A | CYS308 |
| site_id | AC3 |
| Number of Residues | 4 |
| Details | binding site for residue ZN B 401 |
| Chain | Residue |
| B | CYS226 |
| B | CYS229 |
| B | CYS303 |
| B | CYS308 |
Functional Information from PROSITE/UniProt
| site_id | PS00065 |
| Number of Residues | 29 |
| Details | D_2_HYDROXYACID_DH_1 D-isomer specific 2-hydroxyacid dehydrogenases NAD-binding signature. VAIVGvGGVGsvtaemltrcgigkLLlFD |
| Chain | Residue | Details |
| A | VAL76-ASP104 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 12 |
| Details | Motif: {"description":"UFM1-interacting sequence (UIS)","evidences":[{"source":"PubMed","id":"26929408","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"27653677","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 8 |
| Details | Binding site: {"evidences":[{"source":"PubMed","id":"20368332","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"27653677","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"30412706","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"5L95","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"6H77","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"6H78","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI3 |
| Number of Residues | 2 |
| Details | Binding site: {"evidences":[{"source":"PubMed","id":"20368332","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"27653677","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"30412706","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"5L95","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"6H78","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI4 |
| Number of Residues | 8 |
| Details | Binding site: {"evidences":[{"source":"PubMed","id":"20368332","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"27653677","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"30412706","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"5IAA","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5L95","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"6H77","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"6H78","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI5 |
| Number of Residues | 2 |
| Details | Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in UFM1)","evidences":[{"source":"PubMed","id":"25219498","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI6 |
| Number of Residues | 4 |
| Details | Cross-link: {"description":"Glycyl lysine isopeptide (Gly-Lys) (interchain with K-? in acceptor proteins)","evidences":[{"source":"PubMed","id":"15071506","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"25219498","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"27653677","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
