5L95

Crystal structure of human UBA5 in complex with UFM1 and AMP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-09-28
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.979490
Spacegroup name	P 32 2 1
Unit cell lengths	139.875, 139.875, 99.742
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	76.999 - 2.100
R-factor	0.1995
Rwork	0.198
R-free	0.22180
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5iaa
RMSD bond length	0.006
RMSD bond angle	0.884
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2142: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	77.000
High resolution limit [Å]	2.100	2.100
Rmerge	0.155
Number of reflections	65695
<I/σ(I)>	8.5
Completeness [%]	99.8
Redundancy	8.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	0.1M HEPES pH7.5, 10% PEG 6000 and 5% 2-Methyl-2,4-pentanediol (MPD)