5KRU

Crystal structure of Staphylococcal nuclease variant Delta+PHS T62E/V66K at cryogenic temperature

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003676	molecular_function	nucleic acid binding
A	0004518	molecular_function	nuclease activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	18
Details	binding site for residue THP A 201

Chain	Residue
A	ARG35
A	HOH302
A	HOH304
A	HOH310
A	HOH314
A	HOH321
A	HOH328
A	HOH337
A	HOH343
A	HOH377
A	ASP40
A	LYS84
A	TYR85
A	ARG87
A	LEU89
A	TYR113
A	LYS127
A	CA202

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site_id	AC2
Number of Residues	7
Details	binding site for residue CA A 202

Chain	Residue
A	ASP21
A	ASP40
A	THR41
A	THP201
A	HOH304
A	HOH310
A	HOH322

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site_id	AC3
Number of Residues	4
Details	binding site for residue PO4 A 203

Chain	Residue
A	HIS8
A	GLU10
A	LYS64
A	ARG81

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Functional Information from PROSITE/UniProt

site_id	PS01123
Number of Residues	25
Details	TNASE_1 Thermonuclease family signature 1. DGDTVkLmykgqpmtf.RLllVDtPE

Chain	Residue	Details
A	ASP19-GLU43

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site_id	PS01284
Number of Residues	11
Details	TNASE_2 Thermonuclease family signature 2. DKYGRgLAyIY

Chain	Residue	Details
A	ASP83-TYR93

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	3
Details	ACT_SITE: ACT_SITE => ECO:0000269|PubMed:288045

Chain	Residue	Details
A	ARG35
A	GLU43
A	TYR93

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site_id	SWS_FT_FI2
Number of Residues	3
Details	BINDING:

Chain	Residue	Details
A	ASP21
A	ASP40
A	THR41

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Catalytic Information from CSA

site_id	MCSA1
Number of Residues	6
Details	M-CSA 165

Chain	Residue	Details
A	ASP21	metal ligand
A	ARG35	electrostatic stabiliser, hydrogen bond donor
A	ASP40	metal ligand
A	THR41	metal ligand
A	GLU43	hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor
A	TYR93	electrostatic stabiliser, hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor

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