5KRU
Crystal structure of Staphylococcal nuclease variant Delta+PHS T62E/V66K at cryogenic temperature
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | OTHER |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2016-01-13 |
| Detector | AGILENT ATLAS CCD |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 30.858, 60.412, 34.568 |
| Unit cell angles | 90.00, 98.60, 90.00 |
Refinement procedure
| Resolution | 34.180 - 1.600 |
| R-factor | 0.1821 |
| Rwork | 0.180 |
| R-free | 0.22100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3bdc |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.775 |
| Data reduction software | CrysalisPro (1.171.36.32) |
| Data scaling software | CrysalisPro (1.171.36.32) |
| Phasing software | PHASER (2.5.7) |
| Refinement software | REFMAC (5.8.0124) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.014 | 0.098 |
| Number of reflections | 16640 | |
| <I/σ(I)> | 64.39 | 8.96 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 10 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | 28% MPD, 25 mM potassium phosphate, calcium chloride, pdTp |
