5KQS

Structure of NS5 methyltransferase from Zika virus bound to S-adenosylmethionine and 7-methyl-guanosine-5'-diphosphate

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004482	molecular_function	mRNA 5'-cap (guanine-N7-)-methyltransferase activity
A	0004483	molecular_function	mRNA (nucleoside-2'-O-)-methyltransferase activity
A	0008168	molecular_function	methyltransferase activity
A	0032259	biological_process	methylation

Functional Information from PDB Data

site_id	AC1
Number of Residues	18
Details	binding site for residue M7G A 301

Chain	Residue
A	LYS13
A	ARG213
A	SER215
A	GOL303
A	HOH406
A	HOH415
A	HOH416
A	HOH419
A	HOH449
A	HOH558
A	LEU16
A	ASN17
A	MET19
A	PHE24
A	LYS28
A	SER150
A	SER151
A	SER152

---

site_id	AC2
Number of Residues	5
Details	binding site for residue GOL A 302

Chain	Residue
A	ARG37
A	ARG41
A	SER56
A	ARG57
A	ARG84

---

site_id	AC3
Number of Residues	4
Details	binding site for residue GOL A 303

Chain	Residue
A	ASN17
A	SER152
A	M7G301
A	ACT306

---

site_id	AC4
Number of Residues	7
Details	binding site for residue GOL A 304

Chain	Residue
A	ASP131
A	PHE133
A	ARG163
A	ALA265
A	SAM307
A	HOH409
A	HOH414

---

site_id	AC5
Number of Residues	5
Details	binding site for residue GOL A 305

Chain	Residue
A	TYR119
A	SER261
A	HOH435
A	HOH447
A	HOH577

---

site_id	AC6
Number of Residues	4
Details	binding site for residue ACT A 306

Chain	Residue
A	SER151
A	SER152
A	GOL303
A	HOH443

---

site_id	AC7
Number of Residues	21
Details	binding site for residue SAM A 307

Chain	Residue
A	SER56
A	GLY58
A	GLY81
A	CYS82
A	GLY83
A	GLY86
A	TRP87
A	THR104
A	LYS105
A	VAL130
A	ASP131
A	VAL132
A	PHE133
A	ASP146
A	GOL304
A	PO4308
A	HOH409
A	HOH433
A	HOH452
A	HOH511
A	HOH583

---

site_id	AC8
Number of Residues	6
Details	binding site for residue PO4 A 308

Chain	Residue
A	GLY109
A	HIS110
A	GLU111
A	SAM307
A	HOH403
A	HOH556

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	4
Details	ACT_SITE: For 2'-O-MTase activity => ECO:0000250|UniProtKB:Q6YMS4

Chain	Residue	Details
A	LYS61
A	ASP146
A	LYS182
A	GLU218

---

site_id	SWS_FT_FI2
Number of Residues	3
Details	BINDING: BINDING => ECO:0000269|PubMed:27866982

Chain	Residue	Details
A	LYS13
A	GLU149
A	ARG213

---

site_id	SWS_FT_FI3
Number of Residues	2
Details	BINDING: BINDING => ECO:0000255|PROSITE-ProRule:PRU00924, ECO:0000269|PubMed:27866982

Chain	Residue	Details
A	SER56
A	THR104

---

site_id	SWS_FT_FI4
Number of Residues	5
Details	BINDING: BINDING => ECO:0000255|PROSITE-ProRule:PRU00924, ECO:0000269|PubMed:27633330, ECO:0000269|PubMed:27866982, ECO:0000269|PubMed:28031359

Chain	Residue	Details
A	GLY86
A	TRP87
A	LYS105
A	ASP131
A	VAL132

---

site_id	SWS_FT_FI5
Number of Residues	3
Details	BINDING: BINDING => ECO:0000269|PubMed:27633330, ECO:0000269|PubMed:27866982, ECO:0000269|PubMed:28031359

Chain	Residue	Details
A	HIS110
A	GLU111
A	ASP146

---

site_id	SWS_FT_FI6
Number of Residues	2
Details	BINDING: BINDING => ECO:0000255|PROSITE-ProRule:PRU00924

Chain	Residue	Details
A	ILE147
A	TYR220

---

site_id	SWS_FT_FI7
Number of Residues	7
Details	SITE: mRNA cap binding => ECO:0000255|PROSITE-ProRule:PRU00924

Chain	Residue	Details
A	LYS13
A	ASN17
A	PHE24
A	LYS28
A	SER150
A	ARG213
A	SER215

---

site_id	SWS_FT_FI8
Number of Residues	2
Details	SITE: mRNA cap binding; via carbonyl oxygen => ECO:0000255|PROSITE-ProRule:PRU00924

Chain	Residue	Details
A	LEU16
A	MET19

---

site_id	SWS_FT_FI9
Number of Residues	3
Details	SITE: Essential for 2'-O-methyltransferase activity => ECO:0000255|PROSITE-ProRule:PRU00924

Chain	Residue	Details
A	LYS61
A	LYS182
A	GLU218

---

site_id	SWS_FT_FI10
Number of Residues	1
Details	SITE: Essential for 2'-O-methyltransferase and N-7 methyltransferase activity => ECO:0000255|PROSITE-ProRule:PRU00924

Chain	Residue	Details
A	ASP146

---

site_id	SWS_FT_FI11
Number of Residues	1
Details	MOD_RES: Phosphoserine => ECO:0000250|UniProtKB:P03314

Chain	Residue	Details
A	SER56

---