5KQS

Structure of NS5 methyltransferase from Zika virus bound to S-adenosylmethionine and 7-methyl-guanosine-5'-diphosphate

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004482	molecular_function	mRNA 5'-cap (guanine-N7-)-methyltransferase activity
A	0004483	molecular_function	methyltransferase cap1 activity
A	0008168	molecular_function	methyltransferase activity
A	0032259	biological_process	methylation

Functional Information from PDB Data

site_id	AC1
Number of Residues	18
Details	binding site for residue M7G A 301

Chain	Residue
A	LYS13
A	ARG213
A	SER215
A	GOL303
A	HOH406
A	HOH415
A	HOH416
A	HOH419
A	HOH449
A	HOH558
A	LEU16
A	ASN17
A	MET19
A	PHE24
A	LYS28
A	SER150
A	SER151
A	SER152

---

site_id	AC2
Number of Residues	5
Details	binding site for residue GOL A 302

Chain	Residue
A	ARG37
A	ARG41
A	SER56
A	ARG57
A	ARG84

---

site_id	AC3
Number of Residues	4
Details	binding site for residue GOL A 303

Chain	Residue
A	ASN17
A	SER152
A	M7G301
A	ACT306

---

site_id	AC4
Number of Residues	7
Details	binding site for residue GOL A 304

Chain	Residue
A	ASP131
A	PHE133
A	ARG163
A	ALA265
A	SAM307
A	HOH409
A	HOH414

---

site_id	AC5
Number of Residues	5
Details	binding site for residue GOL A 305

Chain	Residue
A	TYR119
A	SER261
A	HOH435
A	HOH447
A	HOH577

---

site_id	AC6
Number of Residues	4
Details	binding site for residue ACT A 306

Chain	Residue
A	SER151
A	SER152
A	GOL303
A	HOH443

---

site_id	AC7
Number of Residues	21
Details	binding site for residue SAM A 307

Chain	Residue
A	SER56
A	GLY58
A	GLY81
A	CYS82
A	GLY83
A	GLY86
A	TRP87
A	THR104
A	LYS105
A	VAL130
A	ASP131
A	VAL132
A	PHE133
A	ASP146
A	GOL304
A	PO4308
A	HOH409
A	HOH433
A	HOH452
A	HOH511
A	HOH583

---

site_id	AC8
Number of Residues	6
Details	binding site for residue PO4 A 308

Chain	Residue
A	GLY109
A	HIS110
A	GLU111
A	SAM307
A	HOH403
A	HOH556

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	3
Details	Region: {"description":"SUMO-interacting motif (SIM)","evidences":[{"source":"UniProtKB","id":"A0A024B7W1","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	4
Details	Active site: {"description":"For 2'-O-MTase activity","evidences":[{"source":"UniProtKB","id":"Q6YMS4","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	17
Details	Binding site: {"evidences":[{"source":"UniProtKB","id":"A0A024B7W1","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	9
Details	Binding site: {"evidences":[{"source":"PROSITE-ProRule","id":"PRU00924","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	7
Details	Site: {"description":"mRNA cap binding","evidences":[{"source":"PROSITE-ProRule","id":"PRU00924","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	2
Details	Site: {"description":"mRNA cap binding; via carbonyl oxygen","evidences":[{"source":"PROSITE-ProRule","id":"PRU00924","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	3
Details	Site: {"description":"Essential for 2'-O-methyltransferase activity","evidences":[{"source":"PROSITE-ProRule","id":"PRU00924","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI8
Number of Residues	1
Details	Site: {"description":"Essential for 2'-O-methyltransferase and N-7 methyltransferase activity","evidences":[{"source":"PROSITE-ProRule","id":"PRU00924","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI9
Number of Residues	1
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"UniProtKB","id":"P03314","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---