5KNN

Evolutionary gain of alanine mischarging to non-cognate tRNAs with a G4:U69 base pair

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000166	molecular_function	nucleotide binding
A	0003676	molecular_function	nucleic acid binding
A	0004813	molecular_function	alanine-tRNA ligase activity
A	0005524	molecular_function	ATP binding
A	0005737	cellular_component	cytoplasm
A	0006419	biological_process	alanyl-tRNA aminoacylation
B	0000166	molecular_function	nucleotide binding
B	0003676	molecular_function	nucleic acid binding
B	0004813	molecular_function	alanine-tRNA ligase activity
B	0005524	molecular_function	ATP binding
B	0005737	cellular_component	cytoplasm
B	0006419	biological_process	alanyl-tRNA aminoacylation
C	0000166	molecular_function	nucleotide binding
C	0003676	molecular_function	nucleic acid binding
C	0004813	molecular_function	alanine-tRNA ligase activity
C	0005524	molecular_function	ATP binding
C	0005737	cellular_component	cytoplasm
C	0006419	biological_process	alanyl-tRNA aminoacylation
D	0000166	molecular_function	nucleotide binding
D	0003676	molecular_function	nucleic acid binding
D	0004813	molecular_function	alanine-tRNA ligase activity
D	0005524	molecular_function	ATP binding
D	0005737	cellular_component	cytoplasm
D	0006419	biological_process	alanyl-tRNA aminoacylation
E	0000166	molecular_function	nucleotide binding
E	0003676	molecular_function	nucleic acid binding
E	0004813	molecular_function	alanine-tRNA ligase activity
E	0005524	molecular_function	ATP binding
E	0005737	cellular_component	cytoplasm
E	0006419	biological_process	alanyl-tRNA aminoacylation
F	0000166	molecular_function	nucleotide binding
F	0003676	molecular_function	nucleic acid binding
F	0004813	molecular_function	alanine-tRNA ligase activity
F	0005524	molecular_function	ATP binding
F	0005737	cellular_component	cytoplasm
F	0006419	biological_process	alanyl-tRNA aminoacylation
G	0000166	molecular_function	nucleotide binding
G	0003676	molecular_function	nucleic acid binding
G	0004813	molecular_function	alanine-tRNA ligase activity
G	0005524	molecular_function	ATP binding
G	0005737	cellular_component	cytoplasm
G	0006419	biological_process	alanyl-tRNA aminoacylation
H	0000166	molecular_function	nucleotide binding
H	0003676	molecular_function	nucleic acid binding
H	0004813	molecular_function	alanine-tRNA ligase activity
H	0005524	molecular_function	ATP binding
H	0005737	cellular_component	cytoplasm
H	0006419	biological_process	alanyl-tRNA aminoacylation

Functional Information from PDB Data

site_id	AC1
Number of Residues	18
Details	binding site for residue A5A A 500

Chain	Residue
A	ALA44
A	ILE214
A	TRP215
A	ASN216
A	VAL218
A	ASP239
A	THR240
A	GLY241
A	GLY243
A	ARG246
A	ARG77
A	ASP84
A	HIS94
A	HIS95
A	PHE98
A	MET100
A	TRP176
A	GLU213

---

site_id	AC2
Number of Residues	16
Details	binding site for residue A5A B 500

Chain	Residue
B	ALA44
B	ARG77
B	ASP84
B	TYR93
B	HIS94
B	HIS95
B	PHE98
B	MET100
B	TRP176
B	GLU213
B	ILE214
B	ASN216
B	ASP239
B	GLY241
B	GLY243
B	ARG246

---

site_id	AC3
Number of Residues	16
Details	binding site for residue A5A C 500

Chain	Residue
C	ARG77
C	ASP84
C	TYR93
C	HIS95
C	PHE98
C	MET100
C	TRP176
C	GLU213
C	ILE214
C	ASN216
C	VAL218
C	ASP239
C	THR240
C	GLY241
C	GLY243
C	ARG246

---

site_id	AC4
Number of Residues	16
Details	binding site for residue A5A D 500

Chain	Residue
D	ARG77
D	ASP84
D	TYR93
D	HIS95
D	PHE98
D	MET100
D	TRP176
D	GLU213
D	ILE214
D	ASN216
D	VAL218
D	ASP239
D	THR240
D	GLY241
D	GLY243
D	ARG246

---

site_id	AC5
Number of Residues	16
Details	binding site for residue A5A E 500

Chain	Residue
E	ALA44
E	ARG77
E	ASP84
E	HIS95
E	PHE98
E	MET100
E	TRP176
E	GLU213
E	ILE214
E	TRP215
E	ASN216
E	ASP239
E	THR240
E	GLY241
E	GLY243
E	ARG246

---

site_id	AC6
Number of Residues	14
Details	binding site for residue A5A F 500

Chain	Residue
F	ALA44
F	ARG77
F	ASP84
F	HIS95
F	PHE98
F	MET100
F	TRP176
F	GLU213
F	ILE214
F	TRP215
F	ASN216
F	ASP239
F	GLY241
F	ARG246

---

site_id	AC7
Number of Residues	16
Details	binding site for residue A5A G 500

Chain	Residue
G	HIS95
G	PHE98
G	MET100
G	TRP176
G	GLU213
G	ILE214
G	ASN216
G	VAL218
G	ASP239
G	THR240
G	GLY241
G	GLY243
G	ARG246
G	ARG77
G	ASP84
G	TYR93

---

site_id	AC8
Number of Residues	15
Details	binding site for residue A5A H 500

Chain	Residue
H	ARG77
H	ASP84
H	HIS95
H	PHE98
H	MET100
H	TRP176
H	GLU213
H	ILE214
H	ASN216
H	VAL218
H	ASP239
H	THR240
H	GLY241
H	GLY243
H	ARG246

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	56
Details	BINDING: BINDING => ECO:0000269|PubMed:27622773, ECO:0000269|Ref.27, ECO:0007744|PDB:4XEM, ECO:0007744|PDB:4XEO, ECO:0007744|PDB:5KNN

Chain	Residue	Details
A	ARG77
B	TRP176
B	ILE214
B	ASN216
B	ASP239
B	GLY243
C	ARG77
C	HIS95
C	TRP176
C	ILE214
C	ASN216
A	HIS95
C	ASP239
C	GLY243
D	ARG77
D	HIS95
D	TRP176
D	ILE214
D	ASN216
D	ASP239
D	GLY243
E	ARG77
A	TRP176
E	HIS95
E	TRP176
E	ILE214
E	ASN216
E	ASP239
E	GLY243
F	ARG77
F	HIS95
F	TRP176
F	ILE214
A	ILE214
F	ASN216
F	ASP239
F	GLY243
G	ARG77
G	HIS95
G	TRP176
G	ILE214
G	ASN216
G	ASP239
G	GLY243
A	ASN216
H	ARG77
H	HIS95
H	TRP176
H	ILE214
H	ASN216
H	ASP239
H	GLY243
A	ASP239
A	GLY243
B	ARG77
B	HIS95

---

site_id	SWS_FT_FI2
Number of Residues	8
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:23186163

Chain	Residue	Details
A	SER8
B	SER8
C	SER8
D	SER8
E	SER8
F	SER8
G	SER8
H	SER8

---

site_id	SWS_FT_FI3
Number of Residues	8
Details	MOD_RES: N6-acetyllysine => ECO:0007744|PubMed:19608861

Chain	Residue	Details
A	LYS19
B	LYS19
C	LYS19
D	LYS19
E	LYS19
F	LYS19
G	LYS19
H	LYS19

---

site_id	SWS_FT_FI4
Number of Residues	8
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:20068231

Chain	Residue	Details
A	SER399
B	SER399
C	SER399
D	SER399
E	SER399
F	SER399
G	SER399
H	SER399

---