5K4H

Wolinella succinogenes L-asparaginase S121 + L-Glutamic acid

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004067	molecular_function	asparaginase activity
A	0005737	cellular_component	cytoplasm
A	0006520	biological_process	amino acid metabolic process
A	0006528	biological_process	asparagine metabolic process
A	0016787	molecular_function	hydrolase activity
B	0004067	molecular_function	asparaginase activity
B	0005737	cellular_component	cytoplasm
B	0006520	biological_process	amino acid metabolic process
B	0006528	biological_process	asparagine metabolic process
B	0016787	molecular_function	hydrolase activity
C	0004067	molecular_function	asparaginase activity
C	0005737	cellular_component	cytoplasm
C	0006520	biological_process	amino acid metabolic process
C	0006528	biological_process	asparagine metabolic process
C	0016787	molecular_function	hydrolase activity
D	0004067	molecular_function	asparaginase activity
D	0005737	cellular_component	cytoplasm
D	0006520	biological_process	amino acid metabolic process
D	0006528	biological_process	asparagine metabolic process
D	0016787	molecular_function	hydrolase activity
E	0004067	molecular_function	asparaginase activity
E	0005737	cellular_component	cytoplasm
E	0006520	biological_process	amino acid metabolic process
E	0006528	biological_process	asparagine metabolic process
E	0016787	molecular_function	hydrolase activity
F	0004067	molecular_function	asparaginase activity
F	0005737	cellular_component	cytoplasm
F	0006520	biological_process	amino acid metabolic process
F	0006528	biological_process	asparagine metabolic process
F	0016787	molecular_function	hydrolase activity
G	0004067	molecular_function	asparaginase activity
G	0005737	cellular_component	cytoplasm
G	0006520	biological_process	amino acid metabolic process
G	0006528	biological_process	asparagine metabolic process
G	0016787	molecular_function	hydrolase activity
H	0004067	molecular_function	asparaginase activity
H	0005737	cellular_component	cytoplasm
H	0006520	biological_process	amino acid metabolic process
H	0006528	biological_process	asparagine metabolic process
H	0016787	molecular_function	hydrolase activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	10
Details	binding site for residue GLU A 400

Chain	Residue
A	GLY59
B	GLU287
A	SER60
A	GLN61
A	GLY92
A	THR93
A	ASP94
A	ALA118
A	HOH596
B	ASN252

---

site_id	AC2
Number of Residues	11
Details	binding site for residue GLU B 400

Chain	Residue
A	ASN252
A	GLU287
B	GLY59
B	SER60
B	GLN61
B	GLY92
B	THR93
B	ASP94
B	ALA118
B	HOH550
B	HOH569

---

site_id	AC3
Number of Residues	13
Details	binding site for residue GLU C 400

Chain	Residue
C	GLY13
C	THR14
C	GLY59
C	SER60
C	GLN61
C	GLY92
C	THR93
C	ASP94
C	ALA118
C	MET119
C	HOH527
D	ASN252
D	GLU287

---

site_id	AC4
Number of Residues	11
Details	binding site for residue GLU D 400

Chain	Residue
C	ASN252
C	GLU287
D	GLY59
D	SER60
D	GLN61
D	GLY92
D	THR93
D	ASP94
D	ALA118
D	HOH563
D	HOH605

---

site_id	AC5
Number of Residues	12
Details	binding site for residue GLU E 400

Chain	Residue
E	GLY59
E	SER60
E	GLN61
E	GLY92
E	THR93
E	ASP94
E	ALA118
E	HOH504
E	HOH506
E	HOH605
F	ASN252
F	GLU287

---

site_id	AC6
Number of Residues	10
Details	binding site for residue GLU F 400

Chain	Residue
E	ASN252
E	GLU287
F	GLY59
F	SER60
F	GLN61
F	GLY92
F	THR93
F	ASP94
F	ALA118
F	HOH503

---

site_id	AC7
Number of Residues	10
Details	binding site for residue GLU G 400

Chain	Residue
G	GLY59
G	SER60
G	GLN61
G	GLY92
G	THR93
G	ASP94
G	ALA118
G	HOH529
H	ASN252
H	GLU287

---

site_id	AC8
Number of Residues	11
Details	binding site for residue GLU H 400

Chain	Residue
G	GLU287
H	GLY59
H	SER60
H	GLN61
H	GLY92
H	THR93
H	ASP94
H	ALA118
H	HOH522
H	HOH541
H	HOH605

---

Functional Information from PROSITE/UniProt

site_id	PS00144
Number of Residues	9
Details	ASN_GLN_ASE_1 Asparaginase / glutaminase active site signature 1. IlATGGTIA

Chain	Residue	Details
A	ILE8-ALA16

---

site_id	PS00917
Number of Residues	11
Details	ASN_GLN_ASE_2 Asparaginase / glutaminase active site signature 2. AvIitHGTDTM

Chain	Residue	Details
A	ALA86-MET96

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	8
Details	ACT_SITE: O-isoaspartyl threonine intermediate => ECO:0000305

Chain	Residue	Details
A	THR14
B	THR14
C	THR14
D	THR14
E	THR14
F	THR14
G	THR14
H	THR14

---

site_id	SWS_FT_FI2
Number of Residues	8
Details	BINDING: BINDING => ECO:0000250

Chain	Residue	Details
A	THR93
B	THR93
C	THR93
D	THR93
E	THR93
F	THR93
G	THR93
H	THR93

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