5K4H

Wolinella succinogenes L-asparaginase S121 + L-Glutamic acid

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2015-06-30
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97872
Spacegroup name	P 1
Unit cell lengths	63.538, 84.181, 120.547
Unit cell angles	87.22, 77.76, 70.84

Refinement procedure

Resolution	30.000 - 2.000
R-factor	0.183
Rwork	0.181
R-free	0.22020
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1wsa
RMSD bond length	0.013
RMSD bond angle	1.583
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	29.353	2.110
High resolution limit [Å]	1.990	5.870	1.990
Rmerge	0.054	0.014	0.494
Number of reflections	148378
<I/σ(I)>	11.85	42.11	1.69
Completeness [%]	94.4	93.8	92.6
Redundancy	1.98
CC(1/2)	0.998

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	283	PEG2000 + HEPES 7.5