Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

5JY7

Complex of Mycobacterium smegmatis trehalose synthase with maltokinase

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000023	biological_process	maltose metabolic process
A	0000272	biological_process	polysaccharide catabolic process
A	0003824	molecular_function	catalytic activity
A	0004556	molecular_function	alpha-amylase activity
A	0005509	molecular_function	calcium ion binding
A	0005975	biological_process	carbohydrate metabolic process
A	0005977	biological_process	glycogen metabolic process
A	0005978	biological_process	glycogen biosynthetic process
A	0005991	biological_process	trehalose metabolic process
A	0016798	molecular_function	hydrolase activity, acting on glycosyl bonds
A	0016853	molecular_function	isomerase activity
A	0046872	molecular_function	metal ion binding
A	0047471	molecular_function	maltose alpha-D-glucosyltransferase activity
B	0000023	biological_process	maltose metabolic process
B	0000272	biological_process	polysaccharide catabolic process
B	0003824	molecular_function	catalytic activity
B	0004556	molecular_function	alpha-amylase activity
B	0005509	molecular_function	calcium ion binding
B	0005975	biological_process	carbohydrate metabolic process
B	0005977	biological_process	glycogen metabolic process
B	0005978	biological_process	glycogen biosynthetic process
B	0005991	biological_process	trehalose metabolic process
B	0016798	molecular_function	hydrolase activity, acting on glycosyl bonds
B	0016853	molecular_function	isomerase activity
B	0046872	molecular_function	metal ion binding
B	0047471	molecular_function	maltose alpha-D-glucosyltransferase activity
C	0000023	biological_process	maltose metabolic process
C	0000272	biological_process	polysaccharide catabolic process
C	0003824	molecular_function	catalytic activity
C	0004556	molecular_function	alpha-amylase activity
C	0005509	molecular_function	calcium ion binding
C	0005975	biological_process	carbohydrate metabolic process
C	0005977	biological_process	glycogen metabolic process
C	0005978	biological_process	glycogen biosynthetic process
C	0005991	biological_process	trehalose metabolic process
C	0016798	molecular_function	hydrolase activity, acting on glycosyl bonds
C	0016853	molecular_function	isomerase activity
C	0046872	molecular_function	metal ion binding
C	0047471	molecular_function	maltose alpha-D-glucosyltransferase activity
D	0000023	biological_process	maltose metabolic process
D	0000272	biological_process	polysaccharide catabolic process
D	0003824	molecular_function	catalytic activity
D	0004556	molecular_function	alpha-amylase activity
D	0005509	molecular_function	calcium ion binding
D	0005975	biological_process	carbohydrate metabolic process
D	0005977	biological_process	glycogen metabolic process
D	0005978	biological_process	glycogen biosynthetic process
D	0005991	biological_process	trehalose metabolic process
D	0016798	molecular_function	hydrolase activity, acting on glycosyl bonds
D	0016853	molecular_function	isomerase activity
D	0046872	molecular_function	metal ion binding
D	0047471	molecular_function	maltose alpha-D-glucosyltransferase activity
E	0000023	biological_process	maltose metabolic process
E	0000272	biological_process	polysaccharide catabolic process
E	0003824	molecular_function	catalytic activity
E	0004556	molecular_function	alpha-amylase activity
E	0005509	molecular_function	calcium ion binding
E	0005975	biological_process	carbohydrate metabolic process
E	0005977	biological_process	glycogen metabolic process
E	0005978	biological_process	glycogen biosynthetic process
E	0005991	biological_process	trehalose metabolic process
E	0016798	molecular_function	hydrolase activity, acting on glycosyl bonds
E	0016853	molecular_function	isomerase activity
E	0046872	molecular_function	metal ion binding
E	0047471	molecular_function	maltose alpha-D-glucosyltransferase activity
F	0000023	biological_process	maltose metabolic process
F	0000272	biological_process	polysaccharide catabolic process
F	0003824	molecular_function	catalytic activity
F	0004556	molecular_function	alpha-amylase activity
F	0005509	molecular_function	calcium ion binding
F	0005975	biological_process	carbohydrate metabolic process
F	0005977	biological_process	glycogen metabolic process
F	0005978	biological_process	glycogen biosynthetic process
F	0005991	biological_process	trehalose metabolic process
F	0016798	molecular_function	hydrolase activity, acting on glycosyl bonds
F	0016853	molecular_function	isomerase activity
F	0046872	molecular_function	metal ion binding
F	0047471	molecular_function	maltose alpha-D-glucosyltransferase activity
G	0000023	biological_process	maltose metabolic process
G	0000272	biological_process	polysaccharide catabolic process
G	0003824	molecular_function	catalytic activity
G	0004556	molecular_function	alpha-amylase activity
G	0005509	molecular_function	calcium ion binding
G	0005975	biological_process	carbohydrate metabolic process
G	0005977	biological_process	glycogen metabolic process
G	0005978	biological_process	glycogen biosynthetic process
G	0005991	biological_process	trehalose metabolic process
G	0016798	molecular_function	hydrolase activity, acting on glycosyl bonds
G	0016853	molecular_function	isomerase activity
G	0046872	molecular_function	metal ion binding
G	0047471	molecular_function	maltose alpha-D-glucosyltransferase activity
H	0000023	biological_process	maltose metabolic process
H	0000272	biological_process	polysaccharide catabolic process
H	0003824	molecular_function	catalytic activity
H	0004556	molecular_function	alpha-amylase activity
H	0005509	molecular_function	calcium ion binding
H	0005975	biological_process	carbohydrate metabolic process
H	0005977	biological_process	glycogen metabolic process
H	0005978	biological_process	glycogen biosynthetic process
H	0005991	biological_process	trehalose metabolic process
H	0016798	molecular_function	hydrolase activity, acting on glycosyl bonds
H	0016853	molecular_function	isomerase activity
H	0046872	molecular_function	metal ion binding
H	0047471	molecular_function	maltose alpha-D-glucosyltransferase activity
I	0005524	molecular_function	ATP binding
I	0005978	biological_process	glycogen biosynthetic process
I	0005992	biological_process	trehalose biosynthetic process
I	0016301	molecular_function	kinase activity
I	0016310	biological_process	phosphorylation
I	0046835	biological_process	carbohydrate phosphorylation
J	0005524	molecular_function	ATP binding
J	0005978	biological_process	glycogen biosynthetic process
J	0005992	biological_process	trehalose biosynthetic process
J	0016301	molecular_function	kinase activity
J	0016310	biological_process	phosphorylation
J	0046835	biological_process	carbohydrate phosphorylation
K	0005524	molecular_function	ATP binding
K	0005978	biological_process	glycogen biosynthetic process
K	0005992	biological_process	trehalose biosynthetic process
K	0016301	molecular_function	kinase activity
K	0016310	biological_process	phosphorylation
K	0046835	biological_process	carbohydrate phosphorylation
L	0005524	molecular_function	ATP binding
L	0005978	biological_process	glycogen biosynthetic process
L	0005992	biological_process	trehalose biosynthetic process
L	0016301	molecular_function	kinase activity
L	0016310	biological_process	phosphorylation
L	0046835	biological_process	carbohydrate phosphorylation
M	0005524	molecular_function	ATP binding
M	0005978	biological_process	glycogen biosynthetic process
M	0005992	biological_process	trehalose biosynthetic process
M	0016301	molecular_function	kinase activity
M	0016310	biological_process	phosphorylation
M	0046835	biological_process	carbohydrate phosphorylation
N	0005524	molecular_function	ATP binding
N	0005978	biological_process	glycogen biosynthetic process
N	0005992	biological_process	trehalose biosynthetic process
N	0016301	molecular_function	kinase activity
N	0016310	biological_process	phosphorylation
N	0046835	biological_process	carbohydrate phosphorylation
O	0005524	molecular_function	ATP binding
O	0005978	biological_process	glycogen biosynthetic process
O	0005992	biological_process	trehalose biosynthetic process
O	0016301	molecular_function	kinase activity
O	0016310	biological_process	phosphorylation
O	0046835	biological_process	carbohydrate phosphorylation
P	0005524	molecular_function	ATP binding
P	0005978	biological_process	glycogen biosynthetic process
P	0005992	biological_process	trehalose biosynthetic process
P	0016301	molecular_function	kinase activity
P	0016310	biological_process	phosphorylation
P	0046835	biological_process	carbohydrate phosphorylation

Functional Information from PDB Data

site_id	AC1
Number of Residues	5
Details	binding site for residue CA A 601

Chain	Residue
A	ASN132
A	ASP200
A	TYR234
A	LEU235
A	GLU237

site_id	AC2
Number of Residues	6
Details	binding site for residue CA B 601

Chain	Residue
B	LEU235
B	GLU237
B	ASN132
B	ASP200
B	LEU201
B	TYR234

site_id	AC3
Number of Residues	6
Details	binding site for residue CA C 601

Chain	Residue
C	ASN132
C	ASP200
C	LEU201
C	TYR234
C	LEU235
C	GLU237

site_id	AC4
Number of Residues	6
Details	binding site for residue CA D 601

Chain	Residue
D	ASN132
D	ASP200
D	LEU201
D	TYR234
D	LEU235
D	GLU237

site_id	AC5
Number of Residues	5
Details	binding site for residue CA E 601

Chain	Residue
E	ASN132
E	ASP200
E	LEU201
E	TYR234
E	LEU235

site_id	AC6
Number of Residues	4
Details	binding site for residue CA F 601

Chain	Residue
F	TYR203
F	LEU235
F	PHE236
F	PHE252

site_id	AC7
Number of Residues	6
Details	binding site for residue CA G 601

Chain	Residue
G	ASN132
G	ASP200
G	LEU201
G	TYR234
G	LEU235
G	GLU237

site_id	AC8
Number of Residues	4
Details	binding site for residue CA H 601

Chain	Residue
H	ASN132
H	LEU201
H	TYR203
H	LEU235

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	8
Details	ACT_SITE: Nucleophile => ECO:0000269\|PubMed:21840994

Chain	Residue	Details
A	ASP230
B	ASP230
C	ASP230
D	ASP230
E	ASP230
F	ASP230
G	ASP230
H	ASP230

site_id	SWS_FT_FI2
Number of Residues	8
Details	ACT_SITE: Proton donor => ECO:0000250\|UniProtKB:Q9ZEU2

Chain	Residue	Details
A	GLU272
B	GLU272
C	GLU272
D	GLU272
E	GLU272
F	GLU272
G	GLU272
H	GLU272

site_id	SWS_FT_FI3
Number of Residues	48
Details	BINDING: BINDING => ECO:0000250\|UniProtKB:Q9ZEU2

Chain	Residue	Details
A	ASP90
B	ARG228
B	HIS341
B	ASP342
C	ASP90
C	HIS133
C	GLN198
C	ARG228
C	HIS341
C	ASP342
D	ASP90
A	HIS133
D	HIS133
D	GLN198
D	ARG228
D	HIS341
D	ASP342
E	ASP90
E	HIS133
E	GLN198
E	ARG228
E	HIS341
A	GLN198
E	ASP342
F	ASP90
F	HIS133
F	GLN198
F	ARG228
F	HIS341
F	ASP342
G	ASP90
G	HIS133
G	GLN198
A	ARG228
G	ARG228
G	HIS341
G	ASP342
H	ASP90
H	HIS133
H	GLN198
H	ARG228
H	HIS341
H	ASP342
A	HIS341
A	ASP342
B	ASP90
B	HIS133
B	GLN198

site_id	SWS_FT_FI4
Number of Residues	40
Details	BINDING: BINDING => ECO:0000269\|PubMed:23735230, ECO:0007744\|PDB:3ZO9, ECO:0007744\|PDB:3ZOA

Chain	Residue	Details
A	ASN132
B	GLU237
C	ASN132
C	ASP200
C	TYR234
C	LEU235
C	GLU237
D	ASN132
D	ASP200
D	TYR234
D	LEU235
A	ASP200
D	GLU237
E	ASN132
E	ASP200
E	TYR234
E	LEU235
E	GLU237
F	ASN132
F	ASP200
F	TYR234
F	LEU235
A	TYR234
F	GLU237
G	ASN132
G	ASP200
G	TYR234
G	LEU235
G	GLU237
H	ASN132
H	ASP200
H	TYR234
H	LEU235
A	LEU235
H	GLU237
A	GLU237
B	ASN132
B	ASP200
B	TYR234
B	LEU235

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)