5JY7
Complex of Mycobacterium smegmatis trehalose synthase with maltokinase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-10-01 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97717 |
| Spacegroup name | P 43 |
| Unit cell lengths | 315.680, 315.680, 124.950 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.900 - 3.600 |
| R-factor | 0.2622 |
| Rwork | 0.261 |
| R-free | 0.28120 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3zo9 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.001 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.910 | 3.660 |
| High resolution limit [Å] | 3.600 | 3.600 |
| Rmerge | 0.170 | 1.540 |
| Number of reflections | 142362 | |
| <I/σ(I)> | 9.1 | 1.1 |
| Completeness [%] | 99.8 | 98.1 |
| Redundancy | 7.7 | 7 |
| CC(1/2) | 0.997 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.7 | 292 | 9% v/v polyethylene glycol 8000, 4% v/v glycerol, 200 mM MgCl2 and 0.1 M Tris HCl |
