5JSW

tRNA guanine Transglycosylase (TGT) in co-crystallized complex with 6-amino-2-((((3a'R,6'R,6a'R)-2,2,2',2'-tetramethyldihydro-3a'H-spiro[[1,3]dioxolane-4,4'-furo[3,4-d][1,3]dioxol]-6'-yl)methyl)amino)-1H-imidazo[4,5-g]quinazolin-8(7H)-one

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0002099	biological_process	tRNA wobble guanine modification
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0006400	biological_process	tRNA modification
A	0008033	biological_process	tRNA processing
A	0008479	molecular_function	tRNA-guanosine(34) queuine transglycosylase activity
A	0008616	biological_process	tRNA queuosine(34) biosynthetic process
A	0016740	molecular_function	transferase activity
A	0016757	molecular_function	glycosyltransferase activity
A	0046872	molecular_function	metal ion binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	4
Details	binding site for residue ZN A 401

Chain	Residue
A	CYS318
A	CYS320
A	CYS323
A	HIS349

---

site_id	AC2
Number of Residues	7
Details	binding site for residue EDO A 402

Chain	Residue
A	HOH580
A	HOH710
A	SER17
A	ILE18
A	GLU119
A	HOH503
A	HOH566

---

site_id	AC3
Number of Residues	4
Details	binding site for residue EDO A 403

Chain	Residue
A	ARG38
A	ARG60
A	ALA61
A	GLY63

---

site_id	AC4
Number of Residues	6
Details	binding site for residue EDO A 404

Chain	Residue
A	HIS145
A	GLY148
A	SER149
A	GLN192
A	HOH603
A	HOH652

---

site_id	AC5
Number of Residues	6
Details	binding site for residue EDO A 405

Chain	Residue
A	ARG183
A	PHE223
A	ASP224
A	HOH539
A	HOH607
A	HOH700

---

site_id	AC6
Number of Residues	8
Details	binding site for residue GOL A 406

Chain	Residue
A	PRO56
A	GLU57
A	GLY94
A	TRP95
A	ASP96
A	ARG97
A	LYS325
A	HOH591

---

site_id	AC7
Number of Residues	6
Details	binding site for residue GOL A 407

Chain	Residue
A	LEU311
A	CYS323
A	LYS325
A	TRP326
A	SER327
A	HOH740

---

site_id	AC8
Number of Residues	7
Details	binding site for residue GOL A 408

Chain	Residue
A	GLU317
A	LYS360
A	PHE373
A	PHE377
A	ARG380
A	HOH574
A	HOH715

---

site_id	AC9
Number of Residues	10
Details	binding site for residue GOL A 409

Chain	Residue
A	SER15
A	PHE16
A	GLU119
A	ARG174
A	ARG177
A	ASP254
A	LYS255
A	HOH503
A	HOH580
A	HOH769

---

site_id	AD1
Number of Residues	6
Details	binding site for residue GOL A 410

Chain	Residue
A	ASN70
A	TYR106
A	GLN107
A	6MM413
A	HOH545
A	HOH568

---

site_id	AD2
Number of Residues	7
Details	binding site for residue GOL A 411

Chain	Residue
A	GLN117
A	ARG174
A	PRO252
A	ASP254
A	LYS255
A	HOH520
A	HOH532

---

site_id	AD3
Number of Residues	6
Details	binding site for residue GOL A 412

Chain	Residue
A	ALA61
A	THR62
A	CYS320
A	ALA321
A	GLU348
A	HOH525

---

site_id	AD4
Number of Residues	16
Details	binding site for residue 6MM A 413

Chain	Residue
A	ASP102
A	SER103
A	TYR106
A	ASP156
A	CYS158
A	ILE201
A	GLN203
A	GLY229
A	GLY230
A	LEU231
A	ALA232
A	MET260
A	GLY261
A	ARG286
A	GOL410
A	HOH506

---

site_id	AD5
Number of Residues	3
Details	binding site for residue CL A 414

Chain	Residue
A	THR285
A	ARG289
A	HOH747

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	6
Details	Region: {"description":"RNA binding","evidences":[{"source":"HAMAP-Rule","id":"MF_00168","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"12949492","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	4
Details	Region: {"description":"RNA binding; important for wobble base 34 recognition","evidences":[{"source":"HAMAP-Rule","id":"MF_00168","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"12949492","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	1
Details	Active site: {"description":"Proton acceptor","evidences":[{"source":"HAMAP-Rule","id":"MF_00168","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"12949492","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	1
Details	Active site: {"description":"Nucleophile","evidences":[{"source":"HAMAP-Rule","id":"MF_00168","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"12949492","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	7
Details	Binding site: {"evidences":[{"source":"HAMAP-Rule","id":"MF_00168","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"12949492","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	4
Details	Binding site: {"evidences":[{"source":"HAMAP-Rule","id":"MF_00168","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"10413112","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"11178905","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"11921407","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"12646024","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"12909636","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"12949492","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"14523925","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"19627989","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"8654383","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"8961936","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

Catalytic Information from CSA

site_id	MCSA1
Number of Residues	6
Details	M-CSA 881

Chain	Residue	Details
A	ASP102	proton shuttle (general acid/base)
A	ASP280	covalent catalysis
A	CYS318	metal ligand
A	CYS320	metal ligand
A	CYS323	metal ligand
A	HIS349	metal ligand

---