5JSW
tRNA guanine Transglycosylase (TGT) in co-crystallized complex with 6-amino-2-((((3a'R,6'R,6a'R)-2,2,2',2'-tetramethyldihydro-3a'H-spiro[[1,3]dioxolane-4,4'-furo[3,4-d][1,3]dioxol]-6'-yl)methyl)amino)-1H-imidazo[4,5-g]quinazolin-8(7H)-one
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P14 (MX2) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-10-13 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9763 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 90.336, 64.956, 70.782 |
Unit cell angles | 90.00, 96.38, 90.00 |
Refinement procedure
Resolution | 30.163 - 1.220 |
R-factor | 0.1256 |
Rwork | 0.125 |
R-free | 0.14390 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1p0d |
RMSD bond length | 0.005 |
RMSD bond angle | 0.847 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.10.1-2155_1492) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.890 | 1.290 |
High resolution limit [Å] | 1.220 | 1.220 |
Rmerge | 0.418 | |
Number of reflections | 114740 | |
<I/σ(I)> | 17.1 | 3 |
Completeness [%] | 95.2 | 91.5 |
Redundancy | 4.2 | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 288 | 13% PEG 8000,100mM MES, 1mM DTT, 10% DMSO |