5J33

Isopropylmalate dehydrogenase in complex with NAD+

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000287	molecular_function	magnesium ion binding
A	0003862	molecular_function	3-isopropylmalate dehydrogenase activity
A	0005829	cellular_component	cytosol
A	0008652	biological_process	amino acid biosynthetic process
A	0009082	biological_process	branched-chain amino acid biosynthetic process
A	0009098	biological_process	L-leucine biosynthetic process
A	0009507	cellular_component	chloroplast
A	0009536	cellular_component	plastid
A	0009553	biological_process	embryo sac development
A	0009555	biological_process	pollen development
A	0009570	cellular_component	chloroplast stroma
A	0009941	cellular_component	chloroplast envelope
A	0016491	molecular_function	oxidoreductase activity
A	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
A	0042803	molecular_function	protein homodimerization activity
A	0046872	molecular_function	metal ion binding
A	0051287	molecular_function	NAD binding
A	0070403	molecular_function	NAD+ binding
B	0000287	molecular_function	magnesium ion binding
B	0003862	molecular_function	3-isopropylmalate dehydrogenase activity
B	0005829	cellular_component	cytosol
B	0008652	biological_process	amino acid biosynthetic process
B	0009082	biological_process	branched-chain amino acid biosynthetic process
B	0009098	biological_process	L-leucine biosynthetic process
B	0009507	cellular_component	chloroplast
B	0009536	cellular_component	plastid
B	0009553	biological_process	embryo sac development
B	0009555	biological_process	pollen development
B	0009570	cellular_component	chloroplast stroma
B	0009941	cellular_component	chloroplast envelope
B	0016491	molecular_function	oxidoreductase activity
B	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
B	0042803	molecular_function	protein homodimerization activity
B	0046872	molecular_function	metal ion binding
B	0051287	molecular_function	NAD binding
B	0070403	molecular_function	NAD+ binding
C	0000287	molecular_function	magnesium ion binding
C	0003862	molecular_function	3-isopropylmalate dehydrogenase activity
C	0005829	cellular_component	cytosol
C	0008652	biological_process	amino acid biosynthetic process
C	0009082	biological_process	branched-chain amino acid biosynthetic process
C	0009098	biological_process	L-leucine biosynthetic process
C	0009507	cellular_component	chloroplast
C	0009536	cellular_component	plastid
C	0009553	biological_process	embryo sac development
C	0009555	biological_process	pollen development
C	0009570	cellular_component	chloroplast stroma
C	0009941	cellular_component	chloroplast envelope
C	0016491	molecular_function	oxidoreductase activity
C	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
C	0042803	molecular_function	protein homodimerization activity
C	0046872	molecular_function	metal ion binding
C	0051287	molecular_function	NAD binding
C	0070403	molecular_function	NAD+ binding
D	0000287	molecular_function	magnesium ion binding
D	0003862	molecular_function	3-isopropylmalate dehydrogenase activity
D	0005829	cellular_component	cytosol
D	0008652	biological_process	amino acid biosynthetic process
D	0009082	biological_process	branched-chain amino acid biosynthetic process
D	0009098	biological_process	L-leucine biosynthetic process
D	0009507	cellular_component	chloroplast
D	0009536	cellular_component	plastid
D	0009553	biological_process	embryo sac development
D	0009555	biological_process	pollen development
D	0009570	cellular_component	chloroplast stroma
D	0009941	cellular_component	chloroplast envelope
D	0016491	molecular_function	oxidoreductase activity
D	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
D	0042803	molecular_function	protein homodimerization activity
D	0046872	molecular_function	metal ion binding
D	0051287	molecular_function	NAD binding
D	0070403	molecular_function	NAD+ binding
E	0000287	molecular_function	magnesium ion binding
E	0003862	molecular_function	3-isopropylmalate dehydrogenase activity
E	0005829	cellular_component	cytosol
E	0008652	biological_process	amino acid biosynthetic process
E	0009082	biological_process	branched-chain amino acid biosynthetic process
E	0009098	biological_process	L-leucine biosynthetic process
E	0009507	cellular_component	chloroplast
E	0009536	cellular_component	plastid
E	0009553	biological_process	embryo sac development
E	0009555	biological_process	pollen development
E	0009570	cellular_component	chloroplast stroma
E	0009941	cellular_component	chloroplast envelope
E	0016491	molecular_function	oxidoreductase activity
E	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
E	0042803	molecular_function	protein homodimerization activity
E	0046872	molecular_function	metal ion binding
E	0051287	molecular_function	NAD binding
E	0070403	molecular_function	NAD+ binding
F	0000287	molecular_function	magnesium ion binding
F	0003862	molecular_function	3-isopropylmalate dehydrogenase activity
F	0005829	cellular_component	cytosol
F	0008652	biological_process	amino acid biosynthetic process
F	0009082	biological_process	branched-chain amino acid biosynthetic process
F	0009098	biological_process	L-leucine biosynthetic process
F	0009507	cellular_component	chloroplast
F	0009536	cellular_component	plastid
F	0009553	biological_process	embryo sac development
F	0009555	biological_process	pollen development
F	0009570	cellular_component	chloroplast stroma
F	0009941	cellular_component	chloroplast envelope
F	0016491	molecular_function	oxidoreductase activity
F	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
F	0042803	molecular_function	protein homodimerization activity
F	0046872	molecular_function	metal ion binding
F	0051287	molecular_function	NAD binding
F	0070403	molecular_function	NAD+ binding
G	0000287	molecular_function	magnesium ion binding
G	0003862	molecular_function	3-isopropylmalate dehydrogenase activity
G	0005829	cellular_component	cytosol
G	0008652	biological_process	amino acid biosynthetic process
G	0009082	biological_process	branched-chain amino acid biosynthetic process
G	0009098	biological_process	L-leucine biosynthetic process
G	0009507	cellular_component	chloroplast
G	0009536	cellular_component	plastid
G	0009553	biological_process	embryo sac development
G	0009555	biological_process	pollen development
G	0009570	cellular_component	chloroplast stroma
G	0009941	cellular_component	chloroplast envelope
G	0016491	molecular_function	oxidoreductase activity
G	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
G	0042803	molecular_function	protein homodimerization activity
G	0046872	molecular_function	metal ion binding
G	0051287	molecular_function	NAD binding
G	0070403	molecular_function	NAD+ binding
H	0000287	molecular_function	magnesium ion binding
H	0003862	molecular_function	3-isopropylmalate dehydrogenase activity
H	0005829	cellular_component	cytosol
H	0008652	biological_process	amino acid biosynthetic process
H	0009082	biological_process	branched-chain amino acid biosynthetic process
H	0009098	biological_process	L-leucine biosynthetic process
H	0009507	cellular_component	chloroplast
H	0009536	cellular_component	plastid
H	0009553	biological_process	embryo sac development
H	0009555	biological_process	pollen development
H	0009570	cellular_component	chloroplast stroma
H	0009941	cellular_component	chloroplast envelope
H	0016491	molecular_function	oxidoreductase activity
H	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
H	0042803	molecular_function	protein homodimerization activity
H	0046872	molecular_function	metal ion binding
H	0051287	molecular_function	NAD binding
H	0070403	molecular_function	NAD+ binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	4
Details	binding site for residue MG A 503

Chain	Residue
A	ASP288
A	ASP292
A	SO4502
B	ASP264

---

site_id	AC2
Number of Residues	4
Details	binding site for residue MG A 506

Chain	Residue
A	ASP264
A	SO4505
B	ASP288
B	ASP292

---

site_id	AC3
Number of Residues	20
Details	binding site for residue NAD C 501

Chain	Residue
C	ALA113
C	ILE114
C	GLU129
C	LEU132
C	ILE301
C	GLU318
C	ILE320
C	HIS321
C	GLY322
C	SER323
C	ALA324
C	ASP326
C	ILE327
C	ALA333
C	ASN334
C	SO4502
C	MG505
H	ASN234
H	TYR262
C	ILE53

---

site_id	AC4
Number of Residues	7
Details	binding site for residue SO4 C 502

Chain	Residue
C	ARG136
C	ARG146
C	ARG174
C	ASP288
C	NAD501
C	MG505
H	LYS232

---

site_id	AC5
Number of Residues	3
Details	binding site for residue SO4 C 503

Chain	Residue
B	GLU392
C	GLU392
C	LYS395

---

site_id	AC6
Number of Residues	4
Details	binding site for residue MG C 504

Chain	Residue
C	ASP264
H	ASP288
H	ASP292
H	SO4503

---

site_id	AC7
Number of Residues	5
Details	binding site for residue MG C 505

Chain	Residue
C	ASP288
C	ASP292
C	NAD501
C	SO4502
H	ASP264

---

site_id	AC8
Number of Residues	4
Details	binding site for residue MG D 505

Chain	Residue
D	ASP264
D	SO4504
F	ASP288
F	ASP292

---

site_id	AC9
Number of Residues	3
Details	binding site for residue MG D 506

Chain	Residue
D	ASP288
D	SO4502
F	ASP264

---

site_id	AD1
Number of Residues	6
Details	binding site for residue MG E 503

Chain	Residue
E	ARG146
E	ASP288
E	ASP292
E	ILE320
E	SO4502
G	ASP264

---

site_id	AD2
Number of Residues	15
Details	binding site for residue NAD G 501

Chain	Residue
E	ASN234
E	TYR262
E	ASN265
G	ILE114
G	GLY116
G	GLU129
G	LEU132
G	HIS321
G	GLY322
G	SER323
G	ALA324
G	PRO325
G	ASP326
G	ASN334
G	SO4502

---

site_id	AD3
Number of Residues	5
Details	binding site for residue SO4 G 502

Chain	Residue
G	ARG136
G	ARG146
G	ARG174
G	ASP288
G	NAD501

---

site_id	AD4
Number of Residues	2
Details	binding site for residue SO4 H 502

Chain	Residue
G	GLU392
H	GLU392

---

site_id	AD5
Number of Residues	24
Details	binding site for residues NAD A 501 and SO4 A 502

Chain	Residue
A	ILE327
A	ASN334
A	ASP375
A	MG503
B	ASN234
B	TYR262
B	ASP264
A	ILE53
A	ALA113
A	ILE114
A	GLU129
A	LEU132
A	ARG136
A	ARG146
A	ARG174
A	ASP288
A	ILE301
A	GLU318
A	PRO319
A	HIS321
A	GLY322
A	SER323
A	ALA324
A	PRO325

---

site_id	AD6
Number of Residues	23
Details	binding site for residues NAD A 504 and SO4 A 505

Chain	Residue
A	TYR262
A	ASP264
A	ASN265
A	MG506
B	ILE53
B	ILE114
B	GLY116
B	GLU129
B	LEU132
B	ARG136
B	ARG146
B	ARG174
B	ASP288
B	ILE301
B	HIS321
B	GLY322
B	SER323
B	ALA324
B	PRO325
B	ASP326
B	ALA333
B	ASN334
B	ASP375

---

site_id	AD7
Number of Residues	19
Details	binding site for residues NAD D 501 and SO4 D 502

Chain	Residue
D	ILE114
D	GLU129
D	LEU132
D	ARG136
D	ARG146
D	ARG174
D	ASP288
D	ILE301
D	GLU318
D	HIS321
D	GLY322
D	SER323
D	ALA324
D	PRO325
D	ASP326
D	ASN334
D	MG506
F	TYR262
F	ASN265

---

site_id	AD8
Number of Residues	20
Details	binding site for residues NAD D 503 and SO4 D 504

Chain	Residue
D	LYS232
D	TYR262
D	ASN265
D	MG505
F	ILE114
F	GLY115
F	GLY116
F	GLU129
F	LEU132
F	ARG136
F	ARG146
F	ARG174
F	ASP288
F	HIS321
F	GLY322
F	SER323
F	ALA324
F	ASP326
F	ASN334
F	ASP375

---

site_id	AD9
Number of Residues	25
Details	binding site for residues NAD E 501 and SO4 E 502

Chain	Residue
E	ILE53
E	ALA113
E	ILE114
E	GLU129
E	LEU132
E	ARG136
E	ARG146
E	ARG174
E	ASP288
E	ILE301
E	GLU318
E	PRO319
E	HIS321
E	GLY322
E	SER323
E	ALA324
E	PRO325
E	ASP326
E	ILE327
E	ALA333
E	ASN334
E	ASP375
E	MG503
G	ASN234
G	TYR262

---

site_id	AE1
Number of Residues	10
Details	binding site for Di-peptide ARG H 186 and ASN H 200

Chain	Residue
C	THR201
C	GLU202
C	LEU236
C	GLU237
C	ALA238
H	ILE180
H	PRO185
H	GLY187
H	PHE199
H	THR201

---

site_id	AE2
Number of Residues	10
Details	binding site for Di-peptide ARG H 186 and ASN H 200

Chain	Residue
C	THR201
C	GLU202
C	LEU236
C	GLU237
C	ALA238
H	ILE180
H	PRO185
H	GLY187
H	PHE199
H	THR201

---

site_id	AE3
Number of Residues	23
Details	binding site for residues NAD H 501 and SO4 H 503

Chain	Residue
C	ASN234
C	TYR262
C	ASP264
C	ASN265
C	MG504
H	ILE53
H	ILE114
H	GLY115
H	GLU129
H	LEU132
H	ARG136
H	ARG146
H	ARG174
H	ASP288
H	GLU318
H	HIS321
H	GLY322
H	SER323
H	ALA324
H	PRO325
H	ASP326
H	ASN334
H	ASP375

---

Functional Information from PROSITE/UniProt

site_id	PS00470
Number of Residues	20
Details	IDH_IMDH Isocitrate and isopropylmalate dehydrogenases signature. NIFGDIlSDeaSmit.GSIGM

Chain	Residue	Details
A	ASN284-MET303

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	264
Details	Binding site: {"evidences":[{"source":"PubMed","id":"27137927","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"5J33","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	32
Details	Binding site: {"evidences":[{"source":"PubMed","id":"27137927","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"5J32","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	16
Details	Binding site: {"evidences":[{"source":"PubMed","id":"27137927","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"5J32","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5J33","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5J34","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	8
Details	Site: {"description":"Confers substrate specificity","evidences":[{"source":"PubMed","id":"21697089","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	16
Details	Site: {"description":"Important for catalysis","evidences":[{"source":"PubMed","id":"27137927","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	16
Details	Site: {"description":"Essential for redox regulation","evidences":[{"source":"UniProtKB","id":"Q9FMT1","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	8
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"UniProtKB","id":"Q9FMT1","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---