5J33
Isopropylmalate dehydrogenase in complex with NAD+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-10-12 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 77.200, 132.868, 349.426 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.729 - 3.492 |
| R-factor | 0.1928 |
| Rwork | 0.189 |
| R-free | 0.26080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3r8w |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.301 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.730 | 3.560 |
| High resolution limit [Å] | 3.490 | 3.490 |
| Rmerge | 0.776 | |
| Number of reflections | 45923 | |
| <I/σ(I)> | 6.4 | 2 |
| Completeness [%] | 98.6 | 58.6 |
| Redundancy | 6.65 | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 1.25 M ammonium sulfate, 0.1 M HEPES, pH7.5, and 5 mM NAD+ |
