5J32
Isopropylmalate dehydrogenase in complex with isopropylmalate
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0000287 | molecular_function | magnesium ion binding |
| A | 0003862 | molecular_function | 3-isopropylmalate dehydrogenase activity |
| A | 0005829 | cellular_component | cytosol |
| A | 0009098 | biological_process | L-leucine biosynthetic process |
| A | 0009507 | cellular_component | chloroplast |
| A | 0009536 | cellular_component | plastid |
| A | 0009553 | biological_process | embryo sac development |
| A | 0009555 | biological_process | pollen development |
| A | 0009570 | cellular_component | chloroplast stroma |
| A | 0009941 | cellular_component | chloroplast envelope |
| A | 0016491 | molecular_function | oxidoreductase activity |
| A | 0016616 | molecular_function | oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor |
| A | 0042803 | molecular_function | protein homodimerization activity |
| A | 0046872 | molecular_function | metal ion binding |
| A | 0051287 | molecular_function | NAD binding |
| A | 0070403 | molecular_function | NAD+ binding |
| B | 0000287 | molecular_function | magnesium ion binding |
| B | 0003862 | molecular_function | 3-isopropylmalate dehydrogenase activity |
| B | 0005829 | cellular_component | cytosol |
| B | 0009098 | biological_process | L-leucine biosynthetic process |
| B | 0009507 | cellular_component | chloroplast |
| B | 0009536 | cellular_component | plastid |
| B | 0009553 | biological_process | embryo sac development |
| B | 0009555 | biological_process | pollen development |
| B | 0009570 | cellular_component | chloroplast stroma |
| B | 0009941 | cellular_component | chloroplast envelope |
| B | 0016491 | molecular_function | oxidoreductase activity |
| B | 0016616 | molecular_function | oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor |
| B | 0042803 | molecular_function | protein homodimerization activity |
| B | 0046872 | molecular_function | metal ion binding |
| B | 0051287 | molecular_function | NAD binding |
| B | 0070403 | molecular_function | NAD+ binding |
| C | 0000287 | molecular_function | magnesium ion binding |
| C | 0003862 | molecular_function | 3-isopropylmalate dehydrogenase activity |
| C | 0005829 | cellular_component | cytosol |
| C | 0009098 | biological_process | L-leucine biosynthetic process |
| C | 0009507 | cellular_component | chloroplast |
| C | 0009536 | cellular_component | plastid |
| C | 0009553 | biological_process | embryo sac development |
| C | 0009555 | biological_process | pollen development |
| C | 0009570 | cellular_component | chloroplast stroma |
| C | 0009941 | cellular_component | chloroplast envelope |
| C | 0016491 | molecular_function | oxidoreductase activity |
| C | 0016616 | molecular_function | oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor |
| C | 0042803 | molecular_function | protein homodimerization activity |
| C | 0046872 | molecular_function | metal ion binding |
| C | 0051287 | molecular_function | NAD binding |
| C | 0070403 | molecular_function | NAD+ binding |
| D | 0000287 | molecular_function | magnesium ion binding |
| D | 0003862 | molecular_function | 3-isopropylmalate dehydrogenase activity |
| D | 0005829 | cellular_component | cytosol |
| D | 0009098 | biological_process | L-leucine biosynthetic process |
| D | 0009507 | cellular_component | chloroplast |
| D | 0009536 | cellular_component | plastid |
| D | 0009553 | biological_process | embryo sac development |
| D | 0009555 | biological_process | pollen development |
| D | 0009570 | cellular_component | chloroplast stroma |
| D | 0009941 | cellular_component | chloroplast envelope |
| D | 0016491 | molecular_function | oxidoreductase activity |
| D | 0016616 | molecular_function | oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor |
| D | 0042803 | molecular_function | protein homodimerization activity |
| D | 0046872 | molecular_function | metal ion binding |
| D | 0051287 | molecular_function | NAD binding |
| D | 0070403 | molecular_function | NAD+ binding |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 6 |
| Details | binding site for residue MG A 501 |
| Chain | Residue |
| A | ASP288 |
| A | ASP292 |
| A | IPM502 |
| A | HOH623 |
| A | HOH657 |
| B | ASP264 |
| site_id | AC2 |
| Number of Residues | 11 |
| Details | binding site for residue IPM A 502 |
| Chain | Residue |
| A | TYR181 |
| A | ASP288 |
| A | MG501 |
| A | HOH623 |
| A | HOH657 |
| A | HOH785 |
| B | LYS232 |
| B | ASP264 |
| A | ARG136 |
| A | ARG146 |
| A | ARG174 |
| site_id | AC3 |
| Number of Residues | 6 |
| Details | binding site for residue MG A 503 |
| Chain | Residue |
| A | ASP264 |
| A | IPM504 |
| B | ASP288 |
| B | ASP292 |
| B | HOH654 |
| B | HOH655 |
| site_id | AC4 |
| Number of Residues | 12 |
| Details | binding site for residue IPM A 504 |
| Chain | Residue |
| A | LYS232 |
| A | ASN234 |
| A | VAL235 |
| A | ASP264 |
| A | MG503 |
| A | HOH793 |
| B | ARG136 |
| B | ARG146 |
| B | ARG174 |
| B | TYR181 |
| B | ASP288 |
| B | HOH654 |
| site_id | AC5 |
| Number of Residues | 6 |
| Details | binding site for residue MG C 501 |
| Chain | Residue |
| C | ASP288 |
| C | ASP292 |
| C | IPM502 |
| C | HOH622 |
| C | HOH803 |
| D | ASP264 |
| site_id | AC6 |
| Number of Residues | 10 |
| Details | binding site for residue IPM C 502 |
| Chain | Residue |
| C | ARG136 |
| C | ARG146 |
| C | ARG174 |
| C | TYR181 |
| C | ASP288 |
| C | MG501 |
| C | HOH622 |
| C | HOH773 |
| D | LYS232 |
| D | ASP264 |
| site_id | AC7 |
| Number of Residues | 6 |
| Details | binding site for residue MG C 503 |
| Chain | Residue |
| C | ASP264 |
| C | IPM504 |
| C | HOH625 |
| D | ASP288 |
| D | ASP292 |
| D | HOH799 |
| site_id | AC8 |
| Number of Residues | 9 |
| Details | binding site for residue IPM C 504 |
| Chain | Residue |
| C | LYS232 |
| C | ASP264 |
| C | MG503 |
| C | HOH625 |
| D | ARG136 |
| D | ARG146 |
| D | ARG174 |
| D | TYR181 |
| D | ASP288 |
Functional Information from PROSITE/UniProt
| site_id | PS00470 |
| Number of Residues | 20 |
| Details | IDH_IMDH Isocitrate and isopropylmalate dehydrogenases signature. NIFGDIlSDeaSmit.GSIGM |
| Chain | Residue | Details |
| A | ASN284-MET303 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 16 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:27137927, ECO:0007744|PDB:5J33 |
| Chain | Residue | Details |
| A | ILE114 | |
| C | ASN234 | |
| C | ASN265 | |
| C | GLU318 | |
| D | ILE114 | |
| D | ASN234 | |
| D | ASN265 | |
| D | GLU318 | |
| A | ASN234 | |
| A | ASN265 | |
| A | GLU318 | |
| B | ILE114 | |
| B | ASN234 | |
| B | ASN265 | |
| B | GLU318 | |
| C | ILE114 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 16 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:27137927, ECO:0007744|PDB:5J32 |
| Chain | Residue | Details |
| A | ARG136 | |
| C | ARG146 | |
| C | ARG174 | |
| C | ASP264 | |
| D | ARG136 | |
| D | ARG146 | |
| D | ARG174 | |
| D | ASP264 | |
| A | ARG146 | |
| A | ARG174 | |
| A | ASP264 | |
| B | ARG136 | |
| B | ARG146 | |
| B | ARG174 | |
| B | ASP264 | |
| C | ARG136 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 8 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:27137927, ECO:0007744|PDB:5J32, ECO:0007744|PDB:5J33, ECO:0007744|PDB:5J34 |
| Chain | Residue | Details |
| A | ASP288 | |
| A | ASP292 | |
| B | ASP288 | |
| B | ASP292 | |
| C | ASP288 | |
| C | ASP292 | |
| D | ASP288 | |
| D | ASP292 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 4 |
| Details | SITE: Confers substrate specificity => ECO:0000269|PubMed:21697089 |
| Chain | Residue | Details |
| A | LEU133 | |
| B | LEU133 | |
| C | LEU133 | |
| D | LEU133 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 8 |
| Details | SITE: Important for catalysis => ECO:0000269|PubMed:27137927 |
| Chain | Residue | Details |
| A | TYR181 | |
| A | LYS232 | |
| B | TYR181 | |
| B | LYS232 | |
| C | TYR181 | |
| C | LYS232 | |
| D | TYR181 | |
| D | LYS232 |
| site_id | SWS_FT_FI6 |
| Number of Residues | 8 |
| Details | SITE: Essential for redox regulation => ECO:0000250|UniProtKB:Q9FMT1 |
| Chain | Residue | Details |
| A | CYS228 | |
| A | CYS386 | |
| B | CYS228 | |
| B | CYS386 | |
| C | CYS228 | |
| C | CYS386 | |
| D | CYS228 | |
| D | CYS386 |
| site_id | SWS_FT_FI7 |
| Number of Residues | 4 |
| Details | MOD_RES: Phosphoserine => ECO:0000250|UniProtKB:Q9FMT1 |
| Chain | Residue | Details |
| A | SER70 | |
| B | SER70 | |
| C | SER70 | |
| D | SER70 |
