5J32
Isopropylmalate dehydrogenase in complex with isopropylmalate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-10-12 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 96.059, 49.622, 158.969 |
| Unit cell angles | 90.00, 105.44, 90.00 |
Refinement procedure
| Resolution | 32.475 - 1.933 |
| R-factor | 0.1607 |
| Rwork | 0.159 |
| R-free | 0.19800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3r8w |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.021 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.500 | 1.960 |
| High resolution limit [Å] | 1.930 | 1.930 |
| Rmerge | 0.624 | |
| Number of reflections | 104824 | |
| <I/σ(I)> | 11 | 2 |
| Completeness [%] | 96.4 | 92.6 |
| Redundancy | 3.15 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 1.0 M ammonium phosphate, 0.1 M imidazole, pH 8.0, and 5 mM isopropylmalate |
