5IL1

Crystal structure of SAM-bound METTL3-METTL14 complex

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0001510	biological_process	RNA methylation
A	0001734	molecular_function	mRNA m(6)A methyltransferase activity
A	0005634	cellular_component	nucleus

Functional Information from PDB Data

site_id	AC1
Number of Residues	20
Details	binding site for residue SAM A 601

Chain	Residue
A	CYS376
A	LYS513
A	PHE534
A	ARG536
A	HIS538
A	ASN539
A	ASN549
A	GLN550
A	HOH739
A	HOH749
A	HOH752
A	ASP377
A	HOH774
A	ILE378
A	ASP395
A	PRO397
A	TYR406
A	GLY407
A	LEU409
A	SER511

---

site_id	AC2
Number of Residues	4
Details	binding site for residue EDO A 602

Chain	Residue
A	ASN445
A	TYR449
A	ARG451
A	HOH764

---

site_id	AC3
Number of Residues	2
Details	binding site for residue EDO B 501

Chain	Residue
A	TRP475
B	ARG254

---

site_id	AC4
Number of Residues	6
Details	binding site for residue EDO B 502

Chain	Residue
B	LEU190
B	GLU192
B	TRP234
B	LYS326
B	ILE330
B	PHE331

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	14
Details	Region: {"description":"Gate loop 1","evidences":[{"source":"PubMed","id":"27281194","evidenceCode":"ECO:0000303"}]}

Chain

Residue

Details

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site_id	SWS_FT_FI2
Number of Residues	20
Details	Region: {"description":"Interaction with METTL14","evidences":[{"source":"PubMed","id":"27281194","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"5IL0","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5IL1","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5IL2","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	17
Details	Region: {"description":"Interphase loop","evidences":[{"source":"PubMed","id":"27281194","evidenceCode":"ECO:0000303"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	23
Details	Region: {"description":"Positively charged region required for RNA-binding","evidences":[{"source":"PubMed","id":"27281194","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	8
Details	Region: {"description":"Gate loop 2","evidences":[{"source":"PubMed","id":"27281194","evidenceCode":"ECO:0000303"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	5
Details	Binding site: {"evidences":[{"source":"PubMed","id":"27281194","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"27373337","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"27627798","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"5IL1","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5IL2","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5K7U","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5K7W","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5L6D","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5L6E","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	2
Details	Binding site: {"evidences":[{"source":"PubMed","id":"27281194","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"27373337","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"5IL1","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5IL2","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5K7U","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5K7W","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI8
Number of Residues	2
Details	Site: {"description":"Interaction with METTL14","evidences":[{"source":"PubMed","id":"27281194","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"5IL1","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5IL2","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI9
Number of Residues	18
Details	Region: {"description":"Interaction with METTL3","evidences":[{"source":"PubMed","id":"27281194","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"5IL0","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5IL1","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5IL2","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI10
Number of Residues	5
Details	Site: {"description":"Interaction with METTL3","evidences":[{"source":"PubMed","id":"27281194","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"5IL0","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5IL1","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5IL2","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI11
Number of Residues	1
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"27281194","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"29348140","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"5IL0","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5IL1","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5IL2","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"24275569","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

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