5GWK
Human topoisomerase IIalpha in complex with DNA and etoposide
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0003677 | molecular_function | DNA binding |
| A | 0003918 | molecular_function | DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity |
| A | 0005524 | molecular_function | ATP binding |
| A | 0006259 | biological_process | DNA metabolic process |
| A | 0006265 | biological_process | DNA topological change |
| B | 0003677 | molecular_function | DNA binding |
| B | 0003918 | molecular_function | DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity |
| B | 0005524 | molecular_function | ATP binding |
| B | 0006259 | biological_process | DNA metabolic process |
| B | 0006265 | biological_process | DNA topological change |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 2 |
| Details | binding site for residue MG A 1301 |
| Chain | Residue |
| A | ASP541 |
| A | ASP543 |
| site_id | AC2 |
| Number of Residues | 3 |
| Details | binding site for residue MG A 1302 |
| Chain | Residue |
| A | ASN851 |
| A | GLU854 |
| A | ARG929 |
| site_id | AC3 |
| Number of Residues | 2 |
| Details | binding site for residue MG B 1301 |
| Chain | Residue |
| B | ASP541 |
| B | ASP543 |
| site_id | AC4 |
| Number of Residues | 2 |
| Details | binding site for residue MG B 1302 |
| Chain | Residue |
| B | ASN851 |
| B | ARG929 |
| site_id | AC5 |
| Number of Residues | 1 |
| Details | binding site for residue MG D 101 |
| Chain | Residue |
| D | DG17 |
| site_id | AC6 |
| Number of Residues | 9 |
| Details | binding site for residue EVP D 102 |
| Chain | Residue |
| B | GLY462 |
| B | ASP463 |
| B | ARG487 |
| B | MET766 |
| D | DA12 |
| D | DG13 |
| E | DC8 |
| F | DT9 |
| F | DG10 |
| site_id | AC7 |
| Number of Residues | 1 |
| Details | binding site for residue MG F 101 |
| Chain | Residue |
| F | DG17 |
| site_id | AC8 |
| Number of Residues | 10 |
| Details | binding site for residue EVP F 102 |
| Chain | Residue |
| A | GLY462 |
| A | ASP463 |
| A | ARG487 |
| A | MET762 |
| A | MET766 |
| C | DC8 |
| D | DT9 |
| D | DG10 |
| F | DA12 |
| F | DG13 |
Functional Information from PROSITE/UniProt
| site_id | PS00177 |
| Number of Residues | 9 |
| Details | TOPOISOMERASE_II DNA topoisomerase II signature. LTEGDSAKT |
| Chain | Residue | Details |
| A | LEU459-THR467 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 234 |
| Details | Domain: {"description":"Toprim","evidences":[{"source":"PROSITE-ProRule","id":"PRU00995","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 18 |
| Details | Region: {"description":"Interaction with DNA","evidences":[{"source":"PubMed","id":"22841979","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI3 |
| Number of Residues | 20 |
| Details | Motif: {"description":"Nuclear export signal"} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI4 |
| Number of Residues | 2 |
| Details | Active site: {"description":"O-(5'-phospho-DNA)-tyrosine intermediate","evidences":[{"source":"PROSITE-ProRule","id":"PRU01384","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI5 |
| Number of Residues | 2 |
| Details | Binding site: {"evidences":[{"source":"PROSITE-ProRule","id":"PRU00995","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI6 |
| Number of Residues | 4 |
| Details | Binding site: {"evidences":[{"source":"PubMed","id":"22841979","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI7 |
| Number of Residues | 2 |
| Details | Site: {"description":"Interaction with DNA","evidences":[{"source":"PROSITE-ProRule","id":"PRU00995","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI8 |
| Number of Residues | 14 |
| Details | Site: {"description":"Interaction with DNA","evidences":[{"source":"PubMed","id":"22841979","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI9 |
| Number of Residues | 2 |
| Details | Site: {"description":"Transition state stabilizer","evidences":[{"source":"UniProtKB","id":"P06786","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI10 |
| Number of Residues | 2 |
| Details | Site: {"description":"Important for DNA bending; intercalates between base pairs of target DNA","evidences":[{"source":"UniProtKB","id":"P06786","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI11 |
| Number of Residues | 22 |
| Details | Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)","evidences":[{"source":"PubMed","id":"28112733","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI12 |
| Number of Residues | 10 |
| Details | Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)","evidences":[{"source":"PubMed","id":"25772364","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"28112733","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
