5GP1

Crystal structure of ZIKV NS5 Methyltransferase in complex with GTP and SAH

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004482	molecular_function	mRNA 5'-cap (guanine-N7-)-methyltransferase activity
A	0004483	molecular_function	methyltransferase cap1 activity
A	0008168	molecular_function	methyltransferase activity
A	0032259	biological_process	methylation
B	0004482	molecular_function	mRNA 5'-cap (guanine-N7-)-methyltransferase activity
B	0004483	molecular_function	methyltransferase cap1 activity
B	0008168	molecular_function	methyltransferase activity
B	0032259	biological_process	methylation
C	0004482	molecular_function	mRNA 5'-cap (guanine-N7-)-methyltransferase activity
C	0004483	molecular_function	methyltransferase cap1 activity
C	0008168	molecular_function	methyltransferase activity
C	0032259	biological_process	methylation

Functional Information from PDB Data

site_id	AC1
Number of Residues	4
Details	binding site for residue SO4 A 301

Chain	Residue
A	LYS29
A	GLU34
A	ARG57
A	ARG213

---

site_id	AC2
Number of Residues	7
Details	binding site for residue SO4 A 302

Chain	Residue
A	ARG84
A	SO4305
A	ARG37
A	ARG41
A	LEU44
A	VAL55
A	ARG57

---

site_id	AC3
Number of Residues	5
Details	binding site for residue SO4 A 303

Chain	Residue
A	GLU155
A	GLU158
A	LEU206
A	SER227
A	SER232

---

site_id	AC4
Number of Residues	2
Details	binding site for residue SO4 A 304

Chain	Residue
A	ARG160
A	LYS252

---

site_id	AC5
Number of Residues	3
Details	binding site for residue SO4 A 305

Chain	Residue
A	ARG84
A	GLU111
A	SO4302

---

site_id	AC6
Number of Residues	11
Details	binding site for residue GTA A 306

Chain	Residue
A	LYS13
A	LEU16
A	ASN17
A	MET19
A	PHE24
A	LYS28
A	SER151
A	SER152
A	SER215
A	HOH413
A	HOH418

---

site_id	AC7
Number of Residues	16
Details	binding site for residue SAH A 307

Chain	Residue
A	SER56
A	GLY58
A	ASP79
A	GLY81
A	CYS82
A	GLY83
A	GLY86
A	TRP87
A	THR104
A	HIS110
A	VAL130
A	ASP131
A	VAL132
A	PHE133
A	ASP146
A	ILE147

---

site_id	AC8
Number of Residues	4
Details	binding site for residue SO4 B 301

Chain	Residue
B	ARG37
B	ARG41
B	ARG57
B	ARG84

---

site_id	AC9
Number of Residues	3
Details	binding site for residue SO4 B 302

Chain	Residue
B	LYS29
B	ARG41
B	ARG213

---

site_id	AD1
Number of Residues	11
Details	binding site for residue GTA B 303

Chain	Residue
B	LYS13
B	LEU16
B	ASN17
B	MET19
B	PHE24
B	LYS28
B	SER150
B	SER151
B	SER152
B	SER215
B	THR216

---

site_id	AD2
Number of Residues	18
Details	binding site for residue SAH B 304

Chain	Residue
B	SER56
B	GLY58
B	GLY81
B	CYS82
B	GLY83
B	ARG84
B	GLY85
B	GLY86
B	TRP87
B	THR104
B	LYS105
B	HIS110
B	GLU111
B	VAL130
B	ASP131
B	VAL132
B	PHE133
B	ASP146

---

site_id	AD3
Number of Residues	3
Details	binding site for residue SO4 C 301

Chain	Residue
C	ARG37
C	ARG57
C	ARG84

---

site_id	AD4
Number of Residues	2
Details	binding site for residue NI C 302

Chain	Residue
C	HIS110
C	GLU111

---

site_id	AD5
Number of Residues	11
Details	binding site for residue GTA C 303

Chain	Residue
C	THR216
C	LYS13
C	LEU16
C	ASN17
C	MET19
C	PHE24
C	LYS28
C	SER150
C	SER151
C	SER152
C	SER215

---

site_id	AD6
Number of Residues	17
Details	binding site for residue SAH C 304

Chain	Residue
C	SER56
C	GLY58
C	GLY81
C	CYS82
C	GLY83
C	GLY85
C	GLY86
C	THR104
C	LYS105
C	HIS110
C	GLU111
C	VAL130
C	ASP131
C	VAL132
C	PHE133
C	ASP146
C	ILE147

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	9
Details	Region: {"description":"SUMO-interacting motif (SIM)","evidences":[{"source":"UniProtKB","id":"A0A024B7W1","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	12
Details	Active site: {"description":"For 2'-O-MTase activity","evidences":[{"source":"UniProtKB","id":"Q6YMS4","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	51
Details	Binding site: {"evidences":[{"source":"UniProtKB","id":"A0A024B7W1","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	27
Details	Binding site: {"evidences":[{"source":"PROSITE-ProRule","id":"PRU00924","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	21
Details	Site: {"description":"mRNA cap binding","evidences":[{"source":"PROSITE-ProRule","id":"PRU00924","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	6
Details	Site: {"description":"mRNA cap binding; via carbonyl oxygen","evidences":[{"source":"PROSITE-ProRule","id":"PRU00924","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	9
Details	Site: {"description":"Essential for 2'-O-methyltransferase activity","evidences":[{"source":"PROSITE-ProRule","id":"PRU00924","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI8
Number of Residues	3
Details	Site: {"description":"Essential for 2'-O-methyltransferase and N-7 methyltransferase activity","evidences":[{"source":"PROSITE-ProRule","id":"PRU00924","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI9
Number of Residues	3
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"UniProtKB","id":"P03314","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---