Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

5FJA

Cryo-EM structure of yeast RNA polymerase III at 4.7 A

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000428	cellular_component	DNA-directed RNA polymerase complex
A	0001056	molecular_function	RNA polymerase III activity
A	0003677	molecular_function	DNA binding
A	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
A	0005515	molecular_function	protein binding
A	0005634	cellular_component	nucleus
A	0005654	cellular_component	nucleoplasm
A	0005666	cellular_component	RNA polymerase III complex
A	0006351	biological_process	DNA-templated transcription
A	0006383	biological_process	transcription by RNA polymerase III
A	0006384	biological_process	transcription initiation at RNA polymerase III promoter
A	0006386	biological_process	termination of RNA polymerase III transcription
A	0008270	molecular_function	zinc ion binding
A	0016740	molecular_function	transferase activity
A	0016779	molecular_function	nucleotidyltransferase activity
A	0034062	molecular_function	5'-3' RNA polymerase activity
A	0042797	biological_process	tRNA transcription by RNA polymerase III
A	0046872	molecular_function	metal ion binding
B	0000428	cellular_component	DNA-directed RNA polymerase complex
B	0001056	molecular_function	RNA polymerase III activity
B	0003677	molecular_function	DNA binding
B	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
B	0005634	cellular_component	nucleus
B	0005654	cellular_component	nucleoplasm
B	0005666	cellular_component	RNA polymerase III complex
B	0006351	biological_process	DNA-templated transcription
B	0006383	biological_process	transcription by RNA polymerase III
B	0006384	biological_process	transcription initiation at RNA polymerase III promoter
B	0006386	biological_process	termination of RNA polymerase III transcription
B	0008270	molecular_function	zinc ion binding
B	0016740	molecular_function	transferase activity
B	0016779	molecular_function	nucleotidyltransferase activity
B	0032549	molecular_function	ribonucleoside binding
B	0034062	molecular_function	5'-3' RNA polymerase activity
B	0042797	biological_process	tRNA transcription by RNA polymerase III
B	0046872	molecular_function	metal ion binding
C	0000428	cellular_component	DNA-directed RNA polymerase complex
C	0001054	molecular_function	RNA polymerase I activity
C	0001056	molecular_function	RNA polymerase III activity
C	0003677	molecular_function	DNA binding
C	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
C	0005515	molecular_function	protein binding
C	0005634	cellular_component	nucleus
C	0005654	cellular_component	nucleoplasm
C	0005666	cellular_component	RNA polymerase III complex
C	0005730	cellular_component	nucleolus
C	0005736	cellular_component	RNA polymerase I complex
C	0006351	biological_process	DNA-templated transcription
C	0006360	biological_process	transcription by RNA polymerase I
C	0006361	biological_process	transcription initiation at RNA polymerase I promoter
C	0006362	biological_process	transcription elongation by RNA polymerase I
C	0006363	biological_process	termination of RNA polymerase I transcription
C	0006383	biological_process	transcription by RNA polymerase III
C	0006384	biological_process	transcription initiation at RNA polymerase III promoter
C	0006386	biological_process	termination of RNA polymerase III transcription
C	0042254	biological_process	ribosome biogenesis
C	0042790	biological_process	nucleolar large rRNA transcription by RNA polymerase I
C	0042797	biological_process	tRNA transcription by RNA polymerase III
C	0046983	molecular_function	protein dimerization activity
C	0055029	cellular_component	nuclear DNA-directed RNA polymerase complex
D	0000166	molecular_function	nucleotide binding
D	0000428	cellular_component	DNA-directed RNA polymerase complex
D	0001056	molecular_function	RNA polymerase III activity
D	0005515	molecular_function	protein binding
D	0005634	cellular_component	nucleus
D	0005654	cellular_component	nucleoplasm
D	0005666	cellular_component	RNA polymerase III complex
D	0005829	cellular_component	cytosol
D	0006352	biological_process	DNA-templated transcription initiation
D	0006383	biological_process	transcription by RNA polymerase III
D	0006384	biological_process	transcription initiation at RNA polymerase III promoter
D	0006386	biological_process	termination of RNA polymerase III transcription
D	0030880	cellular_component	RNA polymerase complex
D	0042797	biological_process	tRNA transcription by RNA polymerase III
D	0044237	biological_process	cellular metabolic process
E	0000428	cellular_component	DNA-directed RNA polymerase complex
E	0001054	molecular_function	RNA polymerase I activity
E	0001055	molecular_function	RNA polymerase II activity
E	0001056	molecular_function	RNA polymerase III activity
E	0001172	biological_process	RNA-templated transcription
E	0003677	molecular_function	DNA binding
E	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
E	0003968	molecular_function	RNA-dependent RNA polymerase activity
E	0005515	molecular_function	protein binding
E	0005634	cellular_component	nucleus
E	0005654	cellular_component	nucleoplasm
E	0005665	cellular_component	RNA polymerase II, core complex
E	0005666	cellular_component	RNA polymerase III complex
E	0005736	cellular_component	RNA polymerase I complex
E	0006351	biological_process	DNA-templated transcription
E	0006360	biological_process	transcription by RNA polymerase I
E	0006361	biological_process	transcription initiation at RNA polymerase I promoter
E	0006362	biological_process	transcription elongation by RNA polymerase I
E	0006363	biological_process	termination of RNA polymerase I transcription
E	0006366	biological_process	transcription by RNA polymerase II
E	0006367	biological_process	transcription initiation at RNA polymerase II promoter
E	0006368	biological_process	transcription elongation by RNA polymerase II
E	0006383	biological_process	transcription by RNA polymerase III
E	0006384	biological_process	transcription initiation at RNA polymerase III promoter
E	0006386	biological_process	termination of RNA polymerase III transcription
E	0042254	biological_process	ribosome biogenesis
E	0042790	biological_process	nucleolar large rRNA transcription by RNA polymerase I
E	0042797	biological_process	tRNA transcription by RNA polymerase III
E	0055029	cellular_component	nuclear DNA-directed RNA polymerase complex
F	0000428	cellular_component	DNA-directed RNA polymerase complex
F	0001054	molecular_function	RNA polymerase I activity
F	0001055	molecular_function	RNA polymerase II activity
F	0001056	molecular_function	RNA polymerase III activity
F	0001172	biological_process	RNA-templated transcription
F	0003677	molecular_function	DNA binding
F	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
F	0003968	molecular_function	RNA-dependent RNA polymerase activity
F	0005634	cellular_component	nucleus
F	0005654	cellular_component	nucleoplasm
F	0005665	cellular_component	RNA polymerase II, core complex
F	0005666	cellular_component	RNA polymerase III complex
F	0005736	cellular_component	RNA polymerase I complex
F	0005737	cellular_component	cytoplasm
F	0006351	biological_process	DNA-templated transcription
F	0006360	biological_process	transcription by RNA polymerase I
F	0006361	biological_process	transcription initiation at RNA polymerase I promoter
F	0006362	biological_process	transcription elongation by RNA polymerase I
F	0006363	biological_process	termination of RNA polymerase I transcription
F	0006366	biological_process	transcription by RNA polymerase II
F	0006367	biological_process	transcription initiation at RNA polymerase II promoter
F	0006368	biological_process	transcription elongation by RNA polymerase II
F	0006383	biological_process	transcription by RNA polymerase III
F	0006384	biological_process	transcription initiation at RNA polymerase III promoter
F	0006386	biological_process	termination of RNA polymerase III transcription
F	0042254	biological_process	ribosome biogenesis
F	0042790	biological_process	nucleolar large rRNA transcription by RNA polymerase I
F	0042797	biological_process	tRNA transcription by RNA polymerase III
G	0000428	cellular_component	DNA-directed RNA polymerase complex
G	0001056	molecular_function	RNA polymerase III activity
G	0003677	molecular_function	DNA binding
G	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
G	0005515	molecular_function	protein binding
G	0005634	cellular_component	nucleus
G	0005654	cellular_component	nucleoplasm
G	0005666	cellular_component	RNA polymerase III complex
G	0006351	biological_process	DNA-templated transcription
G	0006352	biological_process	DNA-templated transcription initiation
G	0006383	biological_process	transcription by RNA polymerase III
G	0006384	biological_process	transcription initiation at RNA polymerase III promoter
G	0006386	biological_process	termination of RNA polymerase III transcription
G	0042797	biological_process	tRNA transcription by RNA polymerase III
G	0055029	cellular_component	nuclear DNA-directed RNA polymerase complex
H	0000428	cellular_component	DNA-directed RNA polymerase complex
H	0001054	molecular_function	RNA polymerase I activity
H	0001055	molecular_function	RNA polymerase II activity
H	0001056	molecular_function	RNA polymerase III activity
H	0001172	biological_process	RNA-templated transcription
H	0003677	molecular_function	DNA binding
H	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
H	0003968	molecular_function	RNA-dependent RNA polymerase activity
H	0005515	molecular_function	protein binding
H	0005634	cellular_component	nucleus
H	0005654	cellular_component	nucleoplasm
H	0005665	cellular_component	RNA polymerase II, core complex
H	0005666	cellular_component	RNA polymerase III complex
H	0005736	cellular_component	RNA polymerase I complex
H	0006351	biological_process	DNA-templated transcription
H	0006360	biological_process	transcription by RNA polymerase I
H	0006361	biological_process	transcription initiation at RNA polymerase I promoter
H	0006362	biological_process	transcription elongation by RNA polymerase I
H	0006363	biological_process	termination of RNA polymerase I transcription
H	0006366	biological_process	transcription by RNA polymerase II
H	0006367	biological_process	transcription initiation at RNA polymerase II promoter
H	0006368	biological_process	transcription elongation by RNA polymerase II
H	0006383	biological_process	transcription by RNA polymerase III
H	0006384	biological_process	transcription initiation at RNA polymerase III promoter
H	0006386	biological_process	termination of RNA polymerase III transcription
H	0042254	biological_process	ribosome biogenesis
H	0042790	biological_process	nucleolar large rRNA transcription by RNA polymerase I
H	0042797	biological_process	tRNA transcription by RNA polymerase III
I	0000428	cellular_component	DNA-directed RNA polymerase complex
I	0001056	molecular_function	RNA polymerase III activity
I	0003676	molecular_function	nucleic acid binding
I	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
I	0005634	cellular_component	nucleus
I	0005654	cellular_component	nucleoplasm
I	0005666	cellular_component	RNA polymerase III complex
I	0005730	cellular_component	nucleolus
I	0006351	biological_process	DNA-templated transcription
I	0006383	biological_process	transcription by RNA polymerase III
I	0006384	biological_process	transcription initiation at RNA polymerase III promoter
I	0006386	biological_process	termination of RNA polymerase III transcription
I	0008270	molecular_function	zinc ion binding
I	0042797	biological_process	tRNA transcription by RNA polymerase III
I	0046872	molecular_function	metal ion binding
I	0055029	cellular_component	nuclear DNA-directed RNA polymerase complex
J	0000428	cellular_component	DNA-directed RNA polymerase complex
J	0001054	molecular_function	RNA polymerase I activity
J	0001055	molecular_function	RNA polymerase II activity
J	0001056	molecular_function	RNA polymerase III activity
J	0001172	biological_process	RNA-templated transcription
J	0003677	molecular_function	DNA binding
J	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
J	0003968	molecular_function	RNA-dependent RNA polymerase activity
J	0005515	molecular_function	protein binding
J	0005634	cellular_component	nucleus
J	0005654	cellular_component	nucleoplasm
J	0005665	cellular_component	RNA polymerase II, core complex
J	0005666	cellular_component	RNA polymerase III complex
J	0005730	cellular_component	nucleolus
J	0005736	cellular_component	RNA polymerase I complex
J	0006351	biological_process	DNA-templated transcription
J	0006360	biological_process	transcription by RNA polymerase I
J	0006361	biological_process	transcription initiation at RNA polymerase I promoter
J	0006362	biological_process	transcription elongation by RNA polymerase I
J	0006363	biological_process	termination of RNA polymerase I transcription
J	0006366	biological_process	transcription by RNA polymerase II
J	0006367	biological_process	transcription initiation at RNA polymerase II promoter
J	0006368	biological_process	transcription elongation by RNA polymerase II
J	0006383	biological_process	transcription by RNA polymerase III
J	0006384	biological_process	transcription initiation at RNA polymerase III promoter
J	0006386	biological_process	termination of RNA polymerase III transcription
J	0008270	molecular_function	zinc ion binding
J	0042254	biological_process	ribosome biogenesis
J	0042790	biological_process	nucleolar large rRNA transcription by RNA polymerase I
J	0042797	biological_process	tRNA transcription by RNA polymerase III
J	0046872	molecular_function	metal ion binding
K	0000428	cellular_component	DNA-directed RNA polymerase complex
K	0001054	molecular_function	RNA polymerase I activity
K	0001056	molecular_function	RNA polymerase III activity
K	0003677	molecular_function	DNA binding
K	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
K	0005515	molecular_function	protein binding
K	0005634	cellular_component	nucleus
K	0005654	cellular_component	nucleoplasm
K	0005666	cellular_component	RNA polymerase III complex
K	0005730	cellular_component	nucleolus
K	0005736	cellular_component	RNA polymerase I complex
K	0006351	biological_process	DNA-templated transcription
K	0006360	biological_process	transcription by RNA polymerase I
K	0006361	biological_process	transcription initiation at RNA polymerase I promoter
K	0006362	biological_process	transcription elongation by RNA polymerase I
K	0006363	biological_process	termination of RNA polymerase I transcription
K	0006383	biological_process	transcription by RNA polymerase III
K	0006384	biological_process	transcription initiation at RNA polymerase III promoter
K	0006386	biological_process	termination of RNA polymerase III transcription
K	0042254	biological_process	ribosome biogenesis
K	0042790	biological_process	nucleolar large rRNA transcription by RNA polymerase I
K	0042797	biological_process	tRNA transcription by RNA polymerase III
K	0046983	molecular_function	protein dimerization activity
K	0055029	cellular_component	nuclear DNA-directed RNA polymerase complex
L	0000428	cellular_component	DNA-directed RNA polymerase complex
L	0001054	molecular_function	RNA polymerase I activity
L	0001055	molecular_function	RNA polymerase II activity
L	0001056	molecular_function	RNA polymerase III activity
L	0001172	biological_process	RNA-templated transcription
L	0003677	molecular_function	DNA binding
L	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
L	0003968	molecular_function	RNA-dependent RNA polymerase activity
L	0005634	cellular_component	nucleus
L	0005654	cellular_component	nucleoplasm
L	0005665	cellular_component	RNA polymerase II, core complex
L	0005666	cellular_component	RNA polymerase III complex
L	0005730	cellular_component	nucleolus
L	0005736	cellular_component	RNA polymerase I complex
L	0005737	cellular_component	cytoplasm
L	0005777	cellular_component	peroxisome
L	0006351	biological_process	DNA-templated transcription
L	0006360	biological_process	transcription by RNA polymerase I
L	0006361	biological_process	transcription initiation at RNA polymerase I promoter
L	0006362	biological_process	transcription elongation by RNA polymerase I
L	0006363	biological_process	termination of RNA polymerase I transcription
L	0006366	biological_process	transcription by RNA polymerase II
L	0006367	biological_process	transcription initiation at RNA polymerase II promoter
L	0006368	biological_process	transcription elongation by RNA polymerase II
L	0006383	biological_process	transcription by RNA polymerase III
L	0006384	biological_process	transcription initiation at RNA polymerase III promoter
L	0006386	biological_process	termination of RNA polymerase III transcription
L	0008270	molecular_function	zinc ion binding
L	0042254	biological_process	ribosome biogenesis
L	0042790	biological_process	nucleolar large rRNA transcription by RNA polymerase I
L	0042797	biological_process	tRNA transcription by RNA polymerase III
L	0046872	molecular_function	metal ion binding
M	0000428	cellular_component	DNA-directed RNA polymerase complex
M	0001056	molecular_function	RNA polymerase III activity
M	0005634	cellular_component	nucleus
M	0005666	cellular_component	RNA polymerase III complex
M	0006351	biological_process	DNA-templated transcription
M	0006383	biological_process	transcription by RNA polymerase III
M	0006384	biological_process	transcription initiation at RNA polymerase III promoter
M	0006386	biological_process	termination of RNA polymerase III transcription
M	0042797	biological_process	tRNA transcription by RNA polymerase III
N	0000428	cellular_component	DNA-directed RNA polymerase complex
N	0001056	molecular_function	RNA polymerase III activity
N	0003677	molecular_function	DNA binding
N	0005634	cellular_component	nucleus
N	0005654	cellular_component	nucleoplasm
N	0005666	cellular_component	RNA polymerase III complex
N	0006383	biological_process	transcription by RNA polymerase III
N	0006384	biological_process	transcription initiation at RNA polymerase III promoter
N	0006386	biological_process	termination of RNA polymerase III transcription
N	0042797	biological_process	tRNA transcription by RNA polymerase III
O	0000428	cellular_component	DNA-directed RNA polymerase complex
O	0001056	molecular_function	RNA polymerase III activity
O	0003677	molecular_function	DNA binding
O	0003697	molecular_function	single-stranded DNA binding
O	0005515	molecular_function	protein binding
O	0005634	cellular_component	nucleus
O	0005654	cellular_component	nucleoplasm
O	0005666	cellular_component	RNA polymerase III complex
O	0005737	cellular_component	cytoplasm
O	0006351	biological_process	DNA-templated transcription
O	0006383	biological_process	transcription by RNA polymerase III
O	0006384	biological_process	transcription initiation at RNA polymerase III promoter
O	0006386	biological_process	termination of RNA polymerase III transcription
O	0042797	biological_process	tRNA transcription by RNA polymerase III
P	0000428	cellular_component	DNA-directed RNA polymerase complex
P	0001056	molecular_function	RNA polymerase III activity
P	0005515	molecular_function	protein binding
P	0005634	cellular_component	nucleus
P	0005654	cellular_component	nucleoplasm
P	0005666	cellular_component	RNA polymerase III complex
P	0005737	cellular_component	cytoplasm
P	0005739	cellular_component	mitochondrion
P	0006383	biological_process	transcription by RNA polymerase III
P	0006384	biological_process	transcription initiation at RNA polymerase III promoter
P	0006386	biological_process	termination of RNA polymerase III transcription
P	0042797	biological_process	tRNA transcription by RNA polymerase III
Q	0006383	biological_process	transcription by RNA polymerase III

Functional Information from PDB Data

site_id	AC1
Number of Residues	5
Details	BINDING SITE FOR RESIDUE ZN A 2000

Chain	Residue
A	CYS67
A	THR69
A	CYS70
A	CYS77
A	HIS80

site_id	AC2
Number of Residues	6
Details	BINDING SITE FOR RESIDUE ZN A 2001

Chain	Residue
A	HIS156
A	CYS157
A	CYS107
A	ASN109
A	CYS110
A	CYS154

site_id	AC3
Number of Residues	5
Details	BINDING SITE FOR RESIDUE ZN B 2000

Chain	Residue
B	CYS1095
B	LYS1097
B	CYS1098
B	CYS1107
B	CYS1110

site_id	AC4
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN I 2000

Chain	Residue
I	CYS5
I	CYS8
I	CYS26
I	CYS29

site_id	AC5
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN J 2000

Chain	Residue
J	CYS7
J	CYS10
J	CYS45
J	CYS46

site_id	AC6
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN L 2000

Chain	Residue
L	CYS31
L	CYS34
L	CYS48
L	CYS51

Functional Information from PROSITE/UniProt

site_id	PS01112
Number of Residues	10
Details	RNA_POL_N_8KD RNA polymerases N / 8 Kd subunits signature. IVPVrCFSCG

Chain	Residue	Details
J	ILE2-GLY11

site_id	PS01030
Number of Residues	26
Details	RNA_POL_M_15KD RNA polymerases M / 15 Kd subunits signature. FCpSCNNMLlitsgdsgvytla.CrsC

Chain	Residue	Details
I	PHE4-CYS29

site_id	PS01111
Number of Residues	15
Details	RNA_POL_K_14KD RNA polymerases K / 14 to 18 Kd subunits signature. TkYErARiLGtRAlQ

Chain	Residue	Details
F	THR86-GLN100

site_id	PS01166
Number of Residues	13
Details	RNA_POL_BETA RNA polymerases beta chain signature. GdKFSSrHGQKGV

Chain	Residue	Details
B	GLY909-VAL921

site_id	PS01154
Number of Residues	32
Details	RNA_POL_L_13KD RNA polymerases L / 13 to 16 Kd subunits signature. IveEdHTLgNaLryvImknpdVefcgYsipHP

Chain	Residue	Details
K	ILE65-PRO96

site_id	PS01110
Number of Residues	14
Details	RNA_POL_H_23KD RNA polymerases H / 23 Kd subunits signature. HELVPKHirLssDE

Chain	Residue	Details
E	HIS147-GLU160

site_id	PS00446
Number of Residues	41
Details	RNA_POL_D_30KD RNA polymerases D / 30 to 40 Kd subunits signature. NAFRRimisevpsvAaeyVyffnNtSviqDEvLAhRIGLVP

Chain	Residue	Details
C	ASN65-PRO105

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1
Details	MOD_RES: Phosphothreonine => ECO:0007744\|PubMed:18407956

Chain	Residue	Details
O	THR27
A	ASP515
N	SER138
J	CYS45
J	CYS46
A	CYS107
A	CYS110
A	CYS154
A	ASP511
A	ASP513

site_id	SWS_FT_FI2
Number of Residues	1
Details	MOD_RES: Phosphoserine => ECO:0007744\|PubMed:19779198

Chain	Residue	Details
O	SER392
N	SER182
L	CYS48
L	CYS51

site_id	SWS_FT_FI3
Number of Residues	1
Details	MOD_RES: Phosphoserine => ECO:0007744\|PubMed:17287358

Chain	Residue	Details
O	SER394
I	CYS75
I	CYS100
I	CYS103

site_id	SWS_FT_FI4
Number of Residues	2
Details	MOD_RES: Phosphothreonine => ECO:0007744\|PubMed:17287358, ECO:0007744\|PubMed:17330950

Chain	Residue	Details
N	THR228
N	THR232

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)