Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

5FHZ

Human aldehyde dehydrogenase 1A3 complexed with NAD(+) and retinoic acid

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0001758	molecular_function	retinal dehydrogenase activity
A	0002072	biological_process	optic cup morphogenesis involved in camera-type eye development
A	0002138	biological_process	retinoic acid biosynthetic process
A	0004029	molecular_function	aldehyde dehydrogenase (NAD+) activity
A	0004030	molecular_function	aldehyde dehydrogenase [NAD(P)+] activity
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0006629	biological_process	lipid metabolic process
A	0006915	biological_process	apoptotic process
A	0007626	biological_process	locomotory behavior
A	0016491	molecular_function	oxidoreductase activity
A	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
A	0021768	biological_process	nucleus accumbens development
A	0031076	biological_process	embryonic camera-type eye development
A	0042472	biological_process	inner ear morphogenesis
A	0042572	biological_process	retinol metabolic process
A	0042573	biological_process	retinoic acid metabolic process
A	0042574	biological_process	retinal metabolic process
A	0042803	molecular_function	protein homodimerization activity
A	0043065	biological_process	positive regulation of apoptotic process
A	0043584	biological_process	nose development
A	0048048	biological_process	embryonic eye morphogenesis
A	0050885	biological_process	neuromuscular process controlling balance
A	0051289	biological_process	protein homotetramerization
A	0060013	biological_process	righting reflex
A	0060166	biological_process	olfactory pit development
A	0060324	biological_process	face development
A	0070062	cellular_component	extracellular exosome
A	0070324	molecular_function	thyroid hormone binding
A	0070384	biological_process	Harderian gland development
A	0070403	molecular_function	NAD+ binding
B	0001758	molecular_function	retinal dehydrogenase activity
B	0002072	biological_process	optic cup morphogenesis involved in camera-type eye development
B	0002138	biological_process	retinoic acid biosynthetic process
B	0004029	molecular_function	aldehyde dehydrogenase (NAD+) activity
B	0004030	molecular_function	aldehyde dehydrogenase [NAD(P)+] activity
B	0005737	cellular_component	cytoplasm
B	0005829	cellular_component	cytosol
B	0006629	biological_process	lipid metabolic process
B	0006915	biological_process	apoptotic process
B	0007626	biological_process	locomotory behavior
B	0016491	molecular_function	oxidoreductase activity
B	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
B	0021768	biological_process	nucleus accumbens development
B	0031076	biological_process	embryonic camera-type eye development
B	0042472	biological_process	inner ear morphogenesis
B	0042572	biological_process	retinol metabolic process
B	0042573	biological_process	retinoic acid metabolic process
B	0042574	biological_process	retinal metabolic process
B	0042803	molecular_function	protein homodimerization activity
B	0043065	biological_process	positive regulation of apoptotic process
B	0043584	biological_process	nose development
B	0048048	biological_process	embryonic eye morphogenesis
B	0050885	biological_process	neuromuscular process controlling balance
B	0051289	biological_process	protein homotetramerization
B	0060013	biological_process	righting reflex
B	0060166	biological_process	olfactory pit development
B	0060324	biological_process	face development
B	0070062	cellular_component	extracellular exosome
B	0070324	molecular_function	thyroid hormone binding
B	0070384	biological_process	Harderian gland development
B	0070403	molecular_function	NAD+ binding
C	0001758	molecular_function	retinal dehydrogenase activity
C	0002072	biological_process	optic cup morphogenesis involved in camera-type eye development
C	0002138	biological_process	retinoic acid biosynthetic process
C	0004029	molecular_function	aldehyde dehydrogenase (NAD+) activity
C	0004030	molecular_function	aldehyde dehydrogenase [NAD(P)+] activity
C	0005737	cellular_component	cytoplasm
C	0005829	cellular_component	cytosol
C	0006629	biological_process	lipid metabolic process
C	0006915	biological_process	apoptotic process
C	0007626	biological_process	locomotory behavior
C	0016491	molecular_function	oxidoreductase activity
C	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
C	0021768	biological_process	nucleus accumbens development
C	0031076	biological_process	embryonic camera-type eye development
C	0042472	biological_process	inner ear morphogenesis
C	0042572	biological_process	retinol metabolic process
C	0042573	biological_process	retinoic acid metabolic process
C	0042574	biological_process	retinal metabolic process
C	0042803	molecular_function	protein homodimerization activity
C	0043065	biological_process	positive regulation of apoptotic process
C	0043584	biological_process	nose development
C	0048048	biological_process	embryonic eye morphogenesis
C	0050885	biological_process	neuromuscular process controlling balance
C	0051289	biological_process	protein homotetramerization
C	0060013	biological_process	righting reflex
C	0060166	biological_process	olfactory pit development
C	0060324	biological_process	face development
C	0070062	cellular_component	extracellular exosome
C	0070324	molecular_function	thyroid hormone binding
C	0070384	biological_process	Harderian gland development
C	0070403	molecular_function	NAD+ binding
D	0001758	molecular_function	retinal dehydrogenase activity
D	0002072	biological_process	optic cup morphogenesis involved in camera-type eye development
D	0002138	biological_process	retinoic acid biosynthetic process
D	0004029	molecular_function	aldehyde dehydrogenase (NAD+) activity
D	0004030	molecular_function	aldehyde dehydrogenase [NAD(P)+] activity
D	0005737	cellular_component	cytoplasm
D	0005829	cellular_component	cytosol
D	0006629	biological_process	lipid metabolic process
D	0006915	biological_process	apoptotic process
D	0007626	biological_process	locomotory behavior
D	0016491	molecular_function	oxidoreductase activity
D	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
D	0021768	biological_process	nucleus accumbens development
D	0031076	biological_process	embryonic camera-type eye development
D	0042472	biological_process	inner ear morphogenesis
D	0042572	biological_process	retinol metabolic process
D	0042573	biological_process	retinoic acid metabolic process
D	0042574	biological_process	retinal metabolic process
D	0042803	molecular_function	protein homodimerization activity
D	0043065	biological_process	positive regulation of apoptotic process
D	0043584	biological_process	nose development
D	0048048	biological_process	embryonic eye morphogenesis
D	0050885	biological_process	neuromuscular process controlling balance
D	0051289	biological_process	protein homotetramerization
D	0060013	biological_process	righting reflex
D	0060166	biological_process	olfactory pit development
D	0060324	biological_process	face development
D	0070062	cellular_component	extracellular exosome
D	0070324	molecular_function	thyroid hormone binding
D	0070384	biological_process	Harderian gland development
D	0070403	molecular_function	NAD+ binding
E	0001758	molecular_function	retinal dehydrogenase activity
E	0002072	biological_process	optic cup morphogenesis involved in camera-type eye development
E	0002138	biological_process	retinoic acid biosynthetic process
E	0004029	molecular_function	aldehyde dehydrogenase (NAD+) activity
E	0004030	molecular_function	aldehyde dehydrogenase [NAD(P)+] activity
E	0005737	cellular_component	cytoplasm
E	0005829	cellular_component	cytosol
E	0006629	biological_process	lipid metabolic process
E	0006915	biological_process	apoptotic process
E	0007626	biological_process	locomotory behavior
E	0016491	molecular_function	oxidoreductase activity
E	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
E	0021768	biological_process	nucleus accumbens development
E	0031076	biological_process	embryonic camera-type eye development
E	0042472	biological_process	inner ear morphogenesis
E	0042572	biological_process	retinol metabolic process
E	0042573	biological_process	retinoic acid metabolic process
E	0042574	biological_process	retinal metabolic process
E	0042803	molecular_function	protein homodimerization activity
E	0043065	biological_process	positive regulation of apoptotic process
E	0043584	biological_process	nose development
E	0048048	biological_process	embryonic eye morphogenesis
E	0050885	biological_process	neuromuscular process controlling balance
E	0051289	biological_process	protein homotetramerization
E	0060013	biological_process	righting reflex
E	0060166	biological_process	olfactory pit development
E	0060324	biological_process	face development
E	0070062	cellular_component	extracellular exosome
E	0070324	molecular_function	thyroid hormone binding
E	0070384	biological_process	Harderian gland development
E	0070403	molecular_function	NAD+ binding
F	0001758	molecular_function	retinal dehydrogenase activity
F	0002072	biological_process	optic cup morphogenesis involved in camera-type eye development
F	0002138	biological_process	retinoic acid biosynthetic process
F	0004029	molecular_function	aldehyde dehydrogenase (NAD+) activity
F	0004030	molecular_function	aldehyde dehydrogenase [NAD(P)+] activity
F	0005737	cellular_component	cytoplasm
F	0005829	cellular_component	cytosol
F	0006629	biological_process	lipid metabolic process
F	0006915	biological_process	apoptotic process
F	0007626	biological_process	locomotory behavior
F	0016491	molecular_function	oxidoreductase activity
F	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
F	0021768	biological_process	nucleus accumbens development
F	0031076	biological_process	embryonic camera-type eye development
F	0042472	biological_process	inner ear morphogenesis
F	0042572	biological_process	retinol metabolic process
F	0042573	biological_process	retinoic acid metabolic process
F	0042574	biological_process	retinal metabolic process
F	0042803	molecular_function	protein homodimerization activity
F	0043065	biological_process	positive regulation of apoptotic process
F	0043584	biological_process	nose development
F	0048048	biological_process	embryonic eye morphogenesis
F	0050885	biological_process	neuromuscular process controlling balance
F	0051289	biological_process	protein homotetramerization
F	0060013	biological_process	righting reflex
F	0060166	biological_process	olfactory pit development
F	0060324	biological_process	face development
F	0070062	cellular_component	extracellular exosome
F	0070324	molecular_function	thyroid hormone binding
F	0070384	biological_process	Harderian gland development
F	0070403	molecular_function	NAD+ binding
G	0001758	molecular_function	retinal dehydrogenase activity
G	0002072	biological_process	optic cup morphogenesis involved in camera-type eye development
G	0002138	biological_process	retinoic acid biosynthetic process
G	0004029	molecular_function	aldehyde dehydrogenase (NAD+) activity
G	0004030	molecular_function	aldehyde dehydrogenase [NAD(P)+] activity
G	0005737	cellular_component	cytoplasm
G	0005829	cellular_component	cytosol
G	0006629	biological_process	lipid metabolic process
G	0006915	biological_process	apoptotic process
G	0007626	biological_process	locomotory behavior
G	0016491	molecular_function	oxidoreductase activity
G	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
G	0021768	biological_process	nucleus accumbens development
G	0031076	biological_process	embryonic camera-type eye development
G	0042472	biological_process	inner ear morphogenesis
G	0042572	biological_process	retinol metabolic process
G	0042573	biological_process	retinoic acid metabolic process
G	0042574	biological_process	retinal metabolic process
G	0042803	molecular_function	protein homodimerization activity
G	0043065	biological_process	positive regulation of apoptotic process
G	0043584	biological_process	nose development
G	0048048	biological_process	embryonic eye morphogenesis
G	0050885	biological_process	neuromuscular process controlling balance
G	0051289	biological_process	protein homotetramerization
G	0060013	biological_process	righting reflex
G	0060166	biological_process	olfactory pit development
G	0060324	biological_process	face development
G	0070062	cellular_component	extracellular exosome
G	0070324	molecular_function	thyroid hormone binding
G	0070384	biological_process	Harderian gland development
G	0070403	molecular_function	NAD+ binding
H	0001758	molecular_function	retinal dehydrogenase activity
H	0002072	biological_process	optic cup morphogenesis involved in camera-type eye development
H	0002138	biological_process	retinoic acid biosynthetic process
H	0004029	molecular_function	aldehyde dehydrogenase (NAD+) activity
H	0004030	molecular_function	aldehyde dehydrogenase [NAD(P)+] activity
H	0005737	cellular_component	cytoplasm
H	0005829	cellular_component	cytosol
H	0006629	biological_process	lipid metabolic process
H	0006915	biological_process	apoptotic process
H	0007626	biological_process	locomotory behavior
H	0016491	molecular_function	oxidoreductase activity
H	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
H	0021768	biological_process	nucleus accumbens development
H	0031076	biological_process	embryonic camera-type eye development
H	0042472	biological_process	inner ear morphogenesis
H	0042572	biological_process	retinol metabolic process
H	0042573	biological_process	retinoic acid metabolic process
H	0042574	biological_process	retinal metabolic process
H	0042803	molecular_function	protein homodimerization activity
H	0043065	biological_process	positive regulation of apoptotic process
H	0043584	biological_process	nose development
H	0048048	biological_process	embryonic eye morphogenesis
H	0050885	biological_process	neuromuscular process controlling balance
H	0051289	biological_process	protein homotetramerization
H	0060013	biological_process	righting reflex
H	0060166	biological_process	olfactory pit development
H	0060324	biological_process	face development
H	0070062	cellular_component	extracellular exosome
H	0070324	molecular_function	thyroid hormone binding
H	0070384	biological_process	Harderian gland development
H	0070403	molecular_function	NAD+ binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	19
Details	binding site for residue NAD A 601

Chain	Residue
A	ILE177
A	PHE255
A	GLY257
A	SER258
A	THR259
A	VAL261
A	GLY282
A	GLN361
A	LYS364
A	GLU411
A	HOH707
A	THR178
A	TRP180
A	LYS204
A	ALA206
A	GLU207
A	GLY237
A	GLY241
A	ALA242

site_id	AC2
Number of Residues	11
Details	binding site for residue REA A 602

Chain	Residue
A	ILE132
A	GLY136
A	ARG139
A	THR140
A	ASN181
A	PHE182
A	MET186
A	CYS313
A	CYS314
A	THR315
A	LEU471

site_id	AC3
Number of Residues	14
Details	binding site for residue NAD B 601

Chain	Residue
B	ILE177
B	THR178
B	TRP180
B	LYS204
B	ALA206
B	GLU207
B	GLN208
B	GLY237
B	GLY241
B	ALA242
B	PHE255
B	SER258
B	VAL261
B	HOH707

site_id	AC4
Number of Residues	6
Details	binding site for residue REA B 602

Chain	Residue
B	THR140
B	LEU185
B	TRP189
B	GLN304
B	LEU471
B	ALA473

site_id	AC5
Number of Residues	17
Details	binding site for residue NAD C 601

Chain	Residue
C	ILE177
C	THR178
C	PRO179
C	TRP180
C	LYS204
C	PRO205
C	ALA206
C	GLU207
C	GLN208
C	GLY237
C	GLY241
C	ALA242
C	PHE255
C	SER258
C	VAL261
C	LYS360
C	LYS364

site_id	AC6
Number of Residues	8
Details	binding site for residue REA C 602

Chain	Residue
C	GLY136
C	THR140
C	LEU185
C	TRP189
C	ASN469
C	LEU471
C	ALA473
C	LEU489

site_id	AC7
Number of Residues	17
Details	binding site for residue NAD D 601

Chain	Residue
D	ILE177
D	THR178
D	TRP180
D	LYS204
D	GLU207
D	GLY237
D	GLY241
D	ALA242
D	PHE255
D	SER258
D	THR259
D	GLU260
D	VAL261
D	GLY282
D	GLN361
D	LYS364
D	GLU411

site_id	AC8
Number of Residues	9
Details	binding site for residue REA D 602

Chain	Residue
D	CYS314
D	LEU471
D	ILE132
D	GLY136
D	ARG139
D	ASN181
D	PHE182
D	MET186
D	CYS313

site_id	AC9
Number of Residues	21
Details	binding site for residue NAD E 601

Chain	Residue
E	THR178
E	PRO179
E	TRP180
E	ASN181
E	LYS204
E	ALA206
E	GLU207
E	GLY237
E	GLY241
E	ALA242
E	PHE255
E	THR256
E	GLY257
E	SER258
E	VAL261
E	LEU281
E	GLY282
E	CYS314
E	GLN361
E	GLU411
E	PHE413

site_id	AD1
Number of Residues	18
Details	binding site for residue NAD F 601

Chain	Residue
F	ILE177
F	THR178
F	TRP180
F	LYS204
F	ALA206
F	GLU207
F	GLY237
F	GLY241
F	ALA242
F	PHE255
F	THR256
F	SER258
F	GLU260
F	VAL261
F	LEU264
F	LYS360
F	GLN361
F	LYS364

site_id	AD2
Number of Residues	17
Details	binding site for residue NAD G 601

Chain	Residue
G	ILE177
G	THR178
G	PRO179
G	TRP180
G	LYS204
G	PRO205
G	ALA206
G	GLU207
G	GLY237
G	GLY241
G	ALA242
G	PHE255
G	SER258
G	GLU260
G	VAL261
G	LYS360
G	LYS364

site_id	AD3
Number of Residues	17
Details	binding site for residue NAD H 601

Chain	Residue
H	ILE177
H	THR178
H	PRO179
H	TRP180
H	LYS204
H	ALA206
H	GLU207
H	GLY237
H	GLY241
H	ALA242
H	PHE255
H	THR256
H	SER258
H	GLU260
H	VAL261
H	LYS360
H	GLN361

Functional Information from PROSITE/UniProt

site_id	PS00070
Number of Residues	12
Details	ALDEHYDE_DEHYDR_CYS Aldehyde dehydrogenases cysteine active site. FfNQGQCCTAAS

Chain	Residue	Details
A	PHE307-SER318

site_id	PS00687
Number of Residues	8
Details	ALDEHYDE_DEHYDR_GLU Aldehyde dehydrogenases glutamic acid active site. LELGGKNP

Chain	Residue	Details
A	LEU279-PRO286

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	8
Details	ACT_SITE: Proton acceptor => ECO:0000255\|PROSITE-ProRule:PRU10007, ECO:0000255\|PROSITE-ProRule:PRU10008, ECO:0000305\|PubMed:27759097

Chain	Residue	Details
A	GLU280
B	GLU280
C	GLU280
D	GLU280
E	GLU280
F	GLU280
G	GLU280
H	GLU280

site_id	SWS_FT_FI2
Number of Residues	8
Details	ACT_SITE: Nucleophile => ECO:0000255\|PROSITE-ProRule:PRU10007, ECO:0000255\|PROSITE-ProRule:PRU10008

Chain	Residue	Details
F	CYS314
G	CYS314
H	CYS314
A	CYS314
B	CYS314
C	CYS314
D	CYS314
E	CYS314

site_id	SWS_FT_FI3
Number of Residues	40
Details	BINDING: BINDING => ECO:0000269\|PubMed:27759097, ECO:0007744\|PDB:5FHZ

Chain	Residue	Details
B	GLN361
B	GLU411
C	LYS204
C	GLU207
C	GLY257
C	GLN361
C	GLU411
D	LYS204
D	GLU207
D	GLY257
D	GLN361
D	GLU411
E	LYS204
E	GLU207
E	GLY257
E	GLN361
E	GLU411
F	LYS204
F	GLU207
F	GLY257
F	GLN361
F	GLU411
G	LYS204
G	GLU207
G	GLY257
G	GLN361
G	GLU411
H	LYS204
H	GLU207
H	GLY257
H	GLN361
H	GLU411
A	LYS204
A	GLU207
A	GLY257
A	GLN361
A	GLU411
B	LYS204
B	GLU207
B	GLY257

site_id	SWS_FT_FI4
Number of Residues	8
Details	SITE: Transition state stabilizer => ECO:0000250

Chain	Residue	Details
F	ASN181
G	ASN181
H	ASN181
A	ASN181
B	ASN181
C	ASN181
D	ASN181
E	ASN181

site_id	SWS_FT_FI5
Number of Residues	8
Details	MOD_RES: N-acetylalanine => ECO:0000269\|Ref.3, ECO:0007744\|PubMed:22814378

Chain	Residue	Details
F	ALA2
G	ALA2
H	ALA2
A	ALA2
B	ALA2
C	ALA2
D	ALA2
E	ALA2

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)