5FHZ

Human aldehyde dehydrogenase 1A3 complexed with NAD(+) and retinoic acid

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0001758	molecular_function	retinal dehydrogenase (NAD+) activity
A	0002138	biological_process	retinoic acid biosynthetic process
A	0004029	molecular_function	aldehyde dehydrogenase (NAD+) activity
A	0004030	molecular_function	aldehyde dehydrogenase [NAD(P)+] activity
A	0005654	cellular_component	nucleoplasm
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0006081	biological_process	aldehyde metabolic process
A	0006629	biological_process	lipid metabolic process
A	0016491	molecular_function	oxidoreductase activity
A	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
A	0031076	biological_process	embryonic camera-type eye development
A	0042572	biological_process	retinol metabolic process
A	0042573	biological_process	retinoic acid metabolic process
A	0042574	biological_process	retinal metabolic process
A	0042803	molecular_function	protein homodimerization activity
A	0043584	biological_process	nose development
A	0048048	biological_process	embryonic eye morphogenesis
A	0051289	biological_process	protein homotetramerization
A	0070062	cellular_component	extracellular exosome
A	0070324	molecular_function	thyroid hormone binding
A	0070384	biological_process	Harderian gland development
A	0070403	molecular_function	NAD+ binding
B	0001758	molecular_function	retinal dehydrogenase (NAD+) activity
B	0002138	biological_process	retinoic acid biosynthetic process
B	0004029	molecular_function	aldehyde dehydrogenase (NAD+) activity
B	0004030	molecular_function	aldehyde dehydrogenase [NAD(P)+] activity
B	0005654	cellular_component	nucleoplasm
B	0005737	cellular_component	cytoplasm
B	0005829	cellular_component	cytosol
B	0006081	biological_process	aldehyde metabolic process
B	0006629	biological_process	lipid metabolic process
B	0016491	molecular_function	oxidoreductase activity
B	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
B	0031076	biological_process	embryonic camera-type eye development
B	0042572	biological_process	retinol metabolic process
B	0042573	biological_process	retinoic acid metabolic process
B	0042574	biological_process	retinal metabolic process
B	0042803	molecular_function	protein homodimerization activity
B	0043584	biological_process	nose development
B	0048048	biological_process	embryonic eye morphogenesis
B	0051289	biological_process	protein homotetramerization
B	0070062	cellular_component	extracellular exosome
B	0070324	molecular_function	thyroid hormone binding
B	0070384	biological_process	Harderian gland development
B	0070403	molecular_function	NAD+ binding
C	0001758	molecular_function	retinal dehydrogenase (NAD+) activity
C	0002138	biological_process	retinoic acid biosynthetic process
C	0004029	molecular_function	aldehyde dehydrogenase (NAD+) activity
C	0004030	molecular_function	aldehyde dehydrogenase [NAD(P)+] activity
C	0005654	cellular_component	nucleoplasm
C	0005737	cellular_component	cytoplasm
C	0005829	cellular_component	cytosol
C	0006081	biological_process	aldehyde metabolic process
C	0006629	biological_process	lipid metabolic process
C	0016491	molecular_function	oxidoreductase activity
C	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
C	0031076	biological_process	embryonic camera-type eye development
C	0042572	biological_process	retinol metabolic process
C	0042573	biological_process	retinoic acid metabolic process
C	0042574	biological_process	retinal metabolic process
C	0042803	molecular_function	protein homodimerization activity
C	0043584	biological_process	nose development
C	0048048	biological_process	embryonic eye morphogenesis
C	0051289	biological_process	protein homotetramerization
C	0070062	cellular_component	extracellular exosome
C	0070324	molecular_function	thyroid hormone binding
C	0070384	biological_process	Harderian gland development
C	0070403	molecular_function	NAD+ binding
D	0001758	molecular_function	retinal dehydrogenase (NAD+) activity
D	0002138	biological_process	retinoic acid biosynthetic process
D	0004029	molecular_function	aldehyde dehydrogenase (NAD+) activity
D	0004030	molecular_function	aldehyde dehydrogenase [NAD(P)+] activity
D	0005654	cellular_component	nucleoplasm
D	0005737	cellular_component	cytoplasm
D	0005829	cellular_component	cytosol
D	0006081	biological_process	aldehyde metabolic process
D	0006629	biological_process	lipid metabolic process
D	0016491	molecular_function	oxidoreductase activity
D	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
D	0031076	biological_process	embryonic camera-type eye development
D	0042572	biological_process	retinol metabolic process
D	0042573	biological_process	retinoic acid metabolic process
D	0042574	biological_process	retinal metabolic process
D	0042803	molecular_function	protein homodimerization activity
D	0043584	biological_process	nose development
D	0048048	biological_process	embryonic eye morphogenesis
D	0051289	biological_process	protein homotetramerization
D	0070062	cellular_component	extracellular exosome
D	0070324	molecular_function	thyroid hormone binding
D	0070384	biological_process	Harderian gland development
D	0070403	molecular_function	NAD+ binding
E	0001758	molecular_function	retinal dehydrogenase (NAD+) activity
E	0002138	biological_process	retinoic acid biosynthetic process
E	0004029	molecular_function	aldehyde dehydrogenase (NAD+) activity
E	0004030	molecular_function	aldehyde dehydrogenase [NAD(P)+] activity
E	0005654	cellular_component	nucleoplasm
E	0005737	cellular_component	cytoplasm
E	0005829	cellular_component	cytosol
E	0006081	biological_process	aldehyde metabolic process
E	0006629	biological_process	lipid metabolic process
E	0016491	molecular_function	oxidoreductase activity
E	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
E	0031076	biological_process	embryonic camera-type eye development
E	0042572	biological_process	retinol metabolic process
E	0042573	biological_process	retinoic acid metabolic process
E	0042574	biological_process	retinal metabolic process
E	0042803	molecular_function	protein homodimerization activity
E	0043584	biological_process	nose development
E	0048048	biological_process	embryonic eye morphogenesis
E	0051289	biological_process	protein homotetramerization
E	0070062	cellular_component	extracellular exosome
E	0070324	molecular_function	thyroid hormone binding
E	0070384	biological_process	Harderian gland development
E	0070403	molecular_function	NAD+ binding
F	0001758	molecular_function	retinal dehydrogenase (NAD+) activity
F	0002138	biological_process	retinoic acid biosynthetic process
F	0004029	molecular_function	aldehyde dehydrogenase (NAD+) activity
F	0004030	molecular_function	aldehyde dehydrogenase [NAD(P)+] activity
F	0005654	cellular_component	nucleoplasm
F	0005737	cellular_component	cytoplasm
F	0005829	cellular_component	cytosol
F	0006081	biological_process	aldehyde metabolic process
F	0006629	biological_process	lipid metabolic process
F	0016491	molecular_function	oxidoreductase activity
F	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
F	0031076	biological_process	embryonic camera-type eye development
F	0042572	biological_process	retinol metabolic process
F	0042573	biological_process	retinoic acid metabolic process
F	0042574	biological_process	retinal metabolic process
F	0042803	molecular_function	protein homodimerization activity
F	0043584	biological_process	nose development
F	0048048	biological_process	embryonic eye morphogenesis
F	0051289	biological_process	protein homotetramerization
F	0070062	cellular_component	extracellular exosome
F	0070324	molecular_function	thyroid hormone binding
F	0070384	biological_process	Harderian gland development
F	0070403	molecular_function	NAD+ binding
G	0001758	molecular_function	retinal dehydrogenase (NAD+) activity
G	0002138	biological_process	retinoic acid biosynthetic process
G	0004029	molecular_function	aldehyde dehydrogenase (NAD+) activity
G	0004030	molecular_function	aldehyde dehydrogenase [NAD(P)+] activity
G	0005654	cellular_component	nucleoplasm
G	0005737	cellular_component	cytoplasm
G	0005829	cellular_component	cytosol
G	0006081	biological_process	aldehyde metabolic process
G	0006629	biological_process	lipid metabolic process
G	0016491	molecular_function	oxidoreductase activity
G	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
G	0031076	biological_process	embryonic camera-type eye development
G	0042572	biological_process	retinol metabolic process
G	0042573	biological_process	retinoic acid metabolic process
G	0042574	biological_process	retinal metabolic process
G	0042803	molecular_function	protein homodimerization activity
G	0043584	biological_process	nose development
G	0048048	biological_process	embryonic eye morphogenesis
G	0051289	biological_process	protein homotetramerization
G	0070062	cellular_component	extracellular exosome
G	0070324	molecular_function	thyroid hormone binding
G	0070384	biological_process	Harderian gland development
G	0070403	molecular_function	NAD+ binding
H	0001758	molecular_function	retinal dehydrogenase (NAD+) activity
H	0002138	biological_process	retinoic acid biosynthetic process
H	0004029	molecular_function	aldehyde dehydrogenase (NAD+) activity
H	0004030	molecular_function	aldehyde dehydrogenase [NAD(P)+] activity
H	0005654	cellular_component	nucleoplasm
H	0005737	cellular_component	cytoplasm
H	0005829	cellular_component	cytosol
H	0006081	biological_process	aldehyde metabolic process
H	0006629	biological_process	lipid metabolic process
H	0016491	molecular_function	oxidoreductase activity
H	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
H	0031076	biological_process	embryonic camera-type eye development
H	0042572	biological_process	retinol metabolic process
H	0042573	biological_process	retinoic acid metabolic process
H	0042574	biological_process	retinal metabolic process
H	0042803	molecular_function	protein homodimerization activity
H	0043584	biological_process	nose development
H	0048048	biological_process	embryonic eye morphogenesis
H	0051289	biological_process	protein homotetramerization
H	0070062	cellular_component	extracellular exosome
H	0070324	molecular_function	thyroid hormone binding
H	0070384	biological_process	Harderian gland development
H	0070403	molecular_function	NAD+ binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	19
Details	binding site for residue NAD A 601

Chain	Residue
A	ILE177
A	PHE255
A	GLY257
A	SER258
A	THR259
A	VAL261
A	GLY282
A	GLN361
A	LYS364
A	GLU411
A	HOH707
A	THR178
A	TRP180
A	LYS204
A	ALA206
A	GLU207
A	GLY237
A	GLY241
A	ALA242

---

site_id	AC2
Number of Residues	11
Details	binding site for residue REA A 602

Chain	Residue
A	ILE132
A	GLY136
A	ARG139
A	THR140
A	ASN181
A	PHE182
A	MET186
A	CYS313
A	CYS314
A	THR315
A	LEU471

---

site_id	AC3
Number of Residues	14
Details	binding site for residue NAD B 601

Chain	Residue
B	ILE177
B	THR178
B	TRP180
B	LYS204
B	ALA206
B	GLU207
B	GLN208
B	GLY237
B	GLY241
B	ALA242
B	PHE255
B	SER258
B	VAL261
B	HOH707

---

site_id	AC4
Number of Residues	6
Details	binding site for residue REA B 602

Chain	Residue
B	THR140
B	LEU185
B	TRP189
B	GLN304
B	LEU471
B	ALA473

---

site_id	AC5
Number of Residues	17
Details	binding site for residue NAD C 601

Chain	Residue
C	ILE177
C	THR178
C	PRO179
C	TRP180
C	LYS204
C	PRO205
C	ALA206
C	GLU207
C	GLN208
C	GLY237
C	GLY241
C	ALA242
C	PHE255
C	SER258
C	VAL261
C	LYS360
C	LYS364

---

site_id	AC6
Number of Residues	8
Details	binding site for residue REA C 602

Chain	Residue
C	GLY136
C	THR140
C	LEU185
C	TRP189
C	ASN469
C	LEU471
C	ALA473
C	LEU489

---

site_id	AC7
Number of Residues	17
Details	binding site for residue NAD D 601

Chain	Residue
D	ILE177
D	THR178
D	TRP180
D	LYS204
D	GLU207
D	GLY237
D	GLY241
D	ALA242
D	PHE255
D	SER258
D	THR259
D	GLU260
D	VAL261
D	GLY282
D	GLN361
D	LYS364
D	GLU411

---

site_id	AC8
Number of Residues	9
Details	binding site for residue REA D 602

Chain	Residue
D	CYS314
D	LEU471
D	ILE132
D	GLY136
D	ARG139
D	ASN181
D	PHE182
D	MET186
D	CYS313

---

site_id	AC9
Number of Residues	21
Details	binding site for residue NAD E 601

Chain	Residue
E	THR178
E	PRO179
E	TRP180
E	ASN181
E	LYS204
E	ALA206
E	GLU207
E	GLY237
E	GLY241
E	ALA242
E	PHE255
E	THR256
E	GLY257
E	SER258
E	VAL261
E	LEU281
E	GLY282
E	CYS314
E	GLN361
E	GLU411
E	PHE413

---

site_id	AD1
Number of Residues	18
Details	binding site for residue NAD F 601

Chain	Residue
F	ILE177
F	THR178
F	TRP180
F	LYS204
F	ALA206
F	GLU207
F	GLY237
F	GLY241
F	ALA242
F	PHE255
F	THR256
F	SER258
F	GLU260
F	VAL261
F	LEU264
F	LYS360
F	GLN361
F	LYS364

---

site_id	AD2
Number of Residues	17
Details	binding site for residue NAD G 601

Chain	Residue
G	ILE177
G	THR178
G	PRO179
G	TRP180
G	LYS204
G	PRO205
G	ALA206
G	GLU207
G	GLY237
G	GLY241
G	ALA242
G	PHE255
G	SER258
G	GLU260
G	VAL261
G	LYS360
G	LYS364

---

site_id	AD3
Number of Residues	17
Details	binding site for residue NAD H 601

Chain	Residue
H	ILE177
H	THR178
H	PRO179
H	TRP180
H	LYS204
H	ALA206
H	GLU207
H	GLY237
H	GLY241
H	ALA242
H	PHE255
H	THR256
H	SER258
H	GLU260
H	VAL261
H	LYS360
H	GLN361

---

Functional Information from PROSITE/UniProt

site_id	PS00070
Number of Residues	12
Details	ALDEHYDE_DEHYDR_CYS Aldehyde dehydrogenases cysteine active site. FfNQGQCCTAAS

Chain	Residue	Details
A	PHE307-SER318

---

site_id	PS00687
Number of Residues	8
Details	ALDEHYDE_DEHYDR_GLU Aldehyde dehydrogenases glutamic acid active site. LELGGKNP

Chain	Residue	Details
A	LEU279-PRO286

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	8
Details	Active site: {"description":"Proton acceptor","evidences":[{"source":"PROSITE-ProRule","id":"PRU10007","evidenceCode":"ECO:0000255"},{"source":"PROSITE-ProRule","id":"PRU10008","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"27759097","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	8
Details	Active site: {"description":"Nucleophile","evidences":[{"source":"PROSITE-ProRule","id":"PRU10007","evidenceCode":"ECO:0000255"},{"source":"PROSITE-ProRule","id":"PRU10008","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	69
Details	Binding site: {"evidences":[{"source":"PubMed","id":"27759097","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"5FHZ","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	8
Details	Site: {"description":"Transition state stabilizer","evidences":[{"evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---