5FHQ

Crystal structure of (WT) Rat Catechol-O-Methyltransferase in complex with AdoMet and 3,5-dinitrocatechol (DNC)

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000287	molecular_function	magnesium ion binding
A	0006584	biological_process	catecholamine metabolic process
A	0008171	molecular_function	O-methyltransferase activity
A	0016206	molecular_function	catechol O-methyltransferase activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	21
Details	binding site for residue SAM A 301

Chain	Residue
A	MET40
A	TYR95
A	GLY117
A	ALA118
A	SER119
A	GLN120
A	ASP141
A	HIS142
A	TRP143
A	DNC302
A	HOH453
A	VAL42
A	HOH489
A	HOH532
A	GLY66
A	ALA67
A	TYR68
A	TYR71
A	SER72
A	GLU90
A	MET91

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site_id	AC2
Number of Residues	11
Details	binding site for residue DNC A 302

Chain	Residue
A	TRP38
A	ASP141
A	HIS142
A	TRP143
A	LYS144
A	ASP169
A	ASN170
A	SAM301
A	MG303
A	HOH416
A	HOH431

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site_id	AC3
Number of Residues	5
Details	binding site for residue MG A 303

Chain	Residue
A	ASP141
A	ASP169
A	ASN170
A	DNC302
A	HOH416

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	4
Details	Binding site: {"evidences":[{"source":"PROSITE-ProRule","id":"PRU01019","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"12237326","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

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site_id	SWS_FT_FI2
Number of Residues	3
Details	Binding site: {"evidences":[{"source":"PROSITE-ProRule","id":"PRU01019","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

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site_id	SWS_FT_FI3
Number of Residues	7
Details	Binding site: {}

Chain

Residue

Details

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Catalytic Information from CSA

site_id	MCSA1
Number of Residues	5
Details	M-CSA 915

Chain	Residue	Details
A	ASP141	metal ligand
A	LYS144	proton shuttle (general acid/base)
A	ASP169	metal ligand
A	ASN170	metal ligand
A	GLU199	electrostatic stabiliser

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