5ES3

Co-crystal structure of LDH liganded with oxamate

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0001666	biological_process	response to hypoxia
A	0001889	biological_process	liver development
A	0003824	molecular_function	catalytic activity
A	0004457	molecular_function	lactate dehydrogenase activity
A	0004459	molecular_function	L-lactate dehydrogenase (NAD+) activity
A	0005515	molecular_function	protein binding
A	0005737	cellular_component	cytoplasm
A	0005739	cellular_component	mitochondrion
A	0005829	cellular_component	cytosol
A	0006089	biological_process	lactate metabolic process
A	0007519	biological_process	skeletal muscle tissue development
A	0007584	biological_process	response to nutrient
A	0009410	biological_process	response to xenobiotic stimulus
A	0009749	biological_process	response to glucose
A	0016491	molecular_function	oxidoreductase activity
A	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
A	0019661	biological_process	glucose catabolic process to lactate via pyruvate
A	0019674	biological_process	NAD+ metabolic process
A	0019752	biological_process	carboxylic acid metabolic process
A	0019900	molecular_function	kinase binding
A	0035686	cellular_component	sperm fibrous sheath
A	0042542	biological_process	response to hydrogen peroxide
A	0042802	molecular_function	identical protein binding
A	0042867	biological_process	pyruvate catabolic process
A	0043065	biological_process	positive regulation of apoptotic process
A	0043627	biological_process	response to estrogen
A	0051287	molecular_function	NAD binding
A	0051591	biological_process	response to cAMP
A	1904628	biological_process	cellular response to phorbol 13-acetate 12-myristate
A	1990204	cellular_component	oxidoreductase complex
B	0001666	biological_process	response to hypoxia
B	0001889	biological_process	liver development
B	0003824	molecular_function	catalytic activity
B	0004457	molecular_function	lactate dehydrogenase activity
B	0004459	molecular_function	L-lactate dehydrogenase (NAD+) activity
B	0005515	molecular_function	protein binding
B	0005737	cellular_component	cytoplasm
B	0005739	cellular_component	mitochondrion
B	0005829	cellular_component	cytosol
B	0006089	biological_process	lactate metabolic process
B	0007519	biological_process	skeletal muscle tissue development
B	0007584	biological_process	response to nutrient
B	0009410	biological_process	response to xenobiotic stimulus
B	0009749	biological_process	response to glucose
B	0016491	molecular_function	oxidoreductase activity
B	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
B	0019661	biological_process	glucose catabolic process to lactate via pyruvate
B	0019674	biological_process	NAD+ metabolic process
B	0019752	biological_process	carboxylic acid metabolic process
B	0019900	molecular_function	kinase binding
B	0035686	cellular_component	sperm fibrous sheath
B	0042542	biological_process	response to hydrogen peroxide
B	0042802	molecular_function	identical protein binding
B	0042867	biological_process	pyruvate catabolic process
B	0043065	biological_process	positive regulation of apoptotic process
B	0043627	biological_process	response to estrogen
B	0051287	molecular_function	NAD binding
B	0051591	biological_process	response to cAMP
B	1904628	biological_process	cellular response to phorbol 13-acetate 12-myristate
B	1990204	cellular_component	oxidoreductase complex
C	0001666	biological_process	response to hypoxia
C	0001889	biological_process	liver development
C	0003824	molecular_function	catalytic activity
C	0004457	molecular_function	lactate dehydrogenase activity
C	0004459	molecular_function	L-lactate dehydrogenase (NAD+) activity
C	0005515	molecular_function	protein binding
C	0005737	cellular_component	cytoplasm
C	0005739	cellular_component	mitochondrion
C	0005829	cellular_component	cytosol
C	0006089	biological_process	lactate metabolic process
C	0007519	biological_process	skeletal muscle tissue development
C	0007584	biological_process	response to nutrient
C	0009410	biological_process	response to xenobiotic stimulus
C	0009749	biological_process	response to glucose
C	0016491	molecular_function	oxidoreductase activity
C	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
C	0019661	biological_process	glucose catabolic process to lactate via pyruvate
C	0019674	biological_process	NAD+ metabolic process
C	0019752	biological_process	carboxylic acid metabolic process
C	0019900	molecular_function	kinase binding
C	0035686	cellular_component	sperm fibrous sheath
C	0042542	biological_process	response to hydrogen peroxide
C	0042802	molecular_function	identical protein binding
C	0042867	biological_process	pyruvate catabolic process
C	0043065	biological_process	positive regulation of apoptotic process
C	0043627	biological_process	response to estrogen
C	0051287	molecular_function	NAD binding
C	0051591	biological_process	response to cAMP
C	1904628	biological_process	cellular response to phorbol 13-acetate 12-myristate
C	1990204	cellular_component	oxidoreductase complex
D	0001666	biological_process	response to hypoxia
D	0001889	biological_process	liver development
D	0003824	molecular_function	catalytic activity
D	0004457	molecular_function	lactate dehydrogenase activity
D	0004459	molecular_function	L-lactate dehydrogenase (NAD+) activity
D	0005515	molecular_function	protein binding
D	0005737	cellular_component	cytoplasm
D	0005739	cellular_component	mitochondrion
D	0005829	cellular_component	cytosol
D	0006089	biological_process	lactate metabolic process
D	0007519	biological_process	skeletal muscle tissue development
D	0007584	biological_process	response to nutrient
D	0009410	biological_process	response to xenobiotic stimulus
D	0009749	biological_process	response to glucose
D	0016491	molecular_function	oxidoreductase activity
D	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
D	0019661	biological_process	glucose catabolic process to lactate via pyruvate
D	0019674	biological_process	NAD+ metabolic process
D	0019752	biological_process	carboxylic acid metabolic process
D	0019900	molecular_function	kinase binding
D	0035686	cellular_component	sperm fibrous sheath
D	0042542	biological_process	response to hydrogen peroxide
D	0042802	molecular_function	identical protein binding
D	0042867	biological_process	pyruvate catabolic process
D	0043065	biological_process	positive regulation of apoptotic process
D	0043627	biological_process	response to estrogen
D	0051287	molecular_function	NAD binding
D	0051591	biological_process	response to cAMP
D	1904628	biological_process	cellular response to phorbol 13-acetate 12-myristate
D	1990204	cellular_component	oxidoreductase complex
E	0001666	biological_process	response to hypoxia
E	0001889	biological_process	liver development
E	0003824	molecular_function	catalytic activity
E	0004457	molecular_function	lactate dehydrogenase activity
E	0004459	molecular_function	L-lactate dehydrogenase (NAD+) activity
E	0005515	molecular_function	protein binding
E	0005737	cellular_component	cytoplasm
E	0005739	cellular_component	mitochondrion
E	0005829	cellular_component	cytosol
E	0006089	biological_process	lactate metabolic process
E	0007519	biological_process	skeletal muscle tissue development
E	0007584	biological_process	response to nutrient
E	0009410	biological_process	response to xenobiotic stimulus
E	0009749	biological_process	response to glucose
E	0016491	molecular_function	oxidoreductase activity
E	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
E	0019661	biological_process	glucose catabolic process to lactate via pyruvate
E	0019674	biological_process	NAD+ metabolic process
E	0019752	biological_process	carboxylic acid metabolic process
E	0019900	molecular_function	kinase binding
E	0035686	cellular_component	sperm fibrous sheath
E	0042542	biological_process	response to hydrogen peroxide
E	0042802	molecular_function	identical protein binding
E	0042867	biological_process	pyruvate catabolic process
E	0043065	biological_process	positive regulation of apoptotic process
E	0043627	biological_process	response to estrogen
E	0051287	molecular_function	NAD binding
E	0051591	biological_process	response to cAMP
E	1904628	biological_process	cellular response to phorbol 13-acetate 12-myristate
E	1990204	cellular_component	oxidoreductase complex
F	0001666	biological_process	response to hypoxia
F	0001889	biological_process	liver development
F	0003824	molecular_function	catalytic activity
F	0004457	molecular_function	lactate dehydrogenase activity
F	0004459	molecular_function	L-lactate dehydrogenase (NAD+) activity
F	0005515	molecular_function	protein binding
F	0005737	cellular_component	cytoplasm
F	0005739	cellular_component	mitochondrion
F	0005829	cellular_component	cytosol
F	0006089	biological_process	lactate metabolic process
F	0007519	biological_process	skeletal muscle tissue development
F	0007584	biological_process	response to nutrient
F	0009410	biological_process	response to xenobiotic stimulus
F	0009749	biological_process	response to glucose
F	0016491	molecular_function	oxidoreductase activity
F	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
F	0019661	biological_process	glucose catabolic process to lactate via pyruvate
F	0019674	biological_process	NAD+ metabolic process
F	0019752	biological_process	carboxylic acid metabolic process
F	0019900	molecular_function	kinase binding
F	0035686	cellular_component	sperm fibrous sheath
F	0042542	biological_process	response to hydrogen peroxide
F	0042802	molecular_function	identical protein binding
F	0042867	biological_process	pyruvate catabolic process
F	0043065	biological_process	positive regulation of apoptotic process
F	0043627	biological_process	response to estrogen
F	0051287	molecular_function	NAD binding
F	0051591	biological_process	response to cAMP
F	1904628	biological_process	cellular response to phorbol 13-acetate 12-myristate
F	1990204	cellular_component	oxidoreductase complex
G	0001666	biological_process	response to hypoxia
G	0001889	biological_process	liver development
G	0003824	molecular_function	catalytic activity
G	0004457	molecular_function	lactate dehydrogenase activity
G	0004459	molecular_function	L-lactate dehydrogenase (NAD+) activity
G	0005515	molecular_function	protein binding
G	0005737	cellular_component	cytoplasm
G	0005739	cellular_component	mitochondrion
G	0005829	cellular_component	cytosol
G	0006089	biological_process	lactate metabolic process
G	0007519	biological_process	skeletal muscle tissue development
G	0007584	biological_process	response to nutrient
G	0009410	biological_process	response to xenobiotic stimulus
G	0009749	biological_process	response to glucose
G	0016491	molecular_function	oxidoreductase activity
G	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
G	0019661	biological_process	glucose catabolic process to lactate via pyruvate
G	0019674	biological_process	NAD+ metabolic process
G	0019752	biological_process	carboxylic acid metabolic process
G	0019900	molecular_function	kinase binding
G	0035686	cellular_component	sperm fibrous sheath
G	0042542	biological_process	response to hydrogen peroxide
G	0042802	molecular_function	identical protein binding
G	0042867	biological_process	pyruvate catabolic process
G	0043065	biological_process	positive regulation of apoptotic process
G	0043627	biological_process	response to estrogen
G	0051287	molecular_function	NAD binding
G	0051591	biological_process	response to cAMP
G	1904628	biological_process	cellular response to phorbol 13-acetate 12-myristate
G	1990204	cellular_component	oxidoreductase complex
H	0001666	biological_process	response to hypoxia
H	0001889	biological_process	liver development
H	0003824	molecular_function	catalytic activity
H	0004457	molecular_function	lactate dehydrogenase activity
H	0004459	molecular_function	L-lactate dehydrogenase (NAD+) activity
H	0005515	molecular_function	protein binding
H	0005737	cellular_component	cytoplasm
H	0005739	cellular_component	mitochondrion
H	0005829	cellular_component	cytosol
H	0006089	biological_process	lactate metabolic process
H	0007519	biological_process	skeletal muscle tissue development
H	0007584	biological_process	response to nutrient
H	0009410	biological_process	response to xenobiotic stimulus
H	0009749	biological_process	response to glucose
H	0016491	molecular_function	oxidoreductase activity
H	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
H	0019661	biological_process	glucose catabolic process to lactate via pyruvate
H	0019674	biological_process	NAD+ metabolic process
H	0019752	biological_process	carboxylic acid metabolic process
H	0019900	molecular_function	kinase binding
H	0035686	cellular_component	sperm fibrous sheath
H	0042542	biological_process	response to hydrogen peroxide
H	0042802	molecular_function	identical protein binding
H	0042867	biological_process	pyruvate catabolic process
H	0043065	biological_process	positive regulation of apoptotic process
H	0043627	biological_process	response to estrogen
H	0051287	molecular_function	NAD binding
H	0051591	biological_process	response to cAMP
H	1904628	biological_process	cellular response to phorbol 13-acetate 12-myristate
H	1990204	cellular_component	oxidoreductase complex

Functional Information from PDB Data

site_id	AC1
Number of Residues	5
Details	binding site for residue OXM A 401

Chain	Residue
A	ASN137
A	ARG168
A	HIS192
A	ALA237
A	THR247

---

site_id	AC2
Number of Residues	5
Details	binding site for residue OXM C 401

Chain	Residue
C	THR247
C	ASN137
C	ARG168
C	HIS192
C	ALA237

---

site_id	AC3
Number of Residues	5
Details	binding site for residue OXM E 401

Chain	Residue
E	ASN137
E	ARG168
E	HIS192
E	ALA237
E	THR247

---

site_id	AC4
Number of Residues	5
Details	binding site for residue OXM F 401

Chain	Residue
F	ASN137
F	ARG168
F	HIS192
F	ALA237
F	THR247

---

site_id	AC5
Number of Residues	5
Details	binding site for residue OXM D 401

Chain	Residue
D	ASN137
D	ARG168
D	HIS192
D	ALA237
D	THR247

---

site_id	AC6
Number of Residues	5
Details	binding site for residue OXM H 401

Chain	Residue
H	ASN137
H	LEU164
H	ARG168
H	HIS192
H	THR247

---

site_id	AC7
Number of Residues	5
Details	binding site for residue OXM B 401

Chain	Residue
B	ASN137
B	ARG168
B	HIS192
B	ALA237
B	THR247

---

site_id	AC8
Number of Residues	6
Details	binding site for residue OXM G 401

Chain	Residue
G	ASN137
G	LEU164
G	ARG168
G	HIS192
G	ALA237
G	THR247

---

Functional Information from PROSITE/UniProt

site_id	PS00064
Number of Residues	7
Details	L_LDH L-lactate dehydrogenase active site. LGEHGDS

Chain	Residue	Details
A	LEU189-SER195

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	8
Details	Active site: {"description":"Proton acceptor","evidences":[{"evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	264
Details	Binding site: {"evidences":[{"evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	20
Details	Modified residue: {"description":"N6-succinyllysine; alternate","evidences":[{"source":"UniProtKB","id":"P06151","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	24
Details	Modified residue: {"description":"N6-acetyllysine","evidences":[{"source":"UniProtKB","id":"P00338","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	8
Details	Modified residue: {"description":"N6-acetyllysine; alternate","evidences":[{"source":"UniProtKB","id":"P00338","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	8
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"22673903","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	24
Details	Modified residue: {"description":"N6-acetyllysine","evidences":[{"source":"UniProtKB","id":"P06151","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI8
Number of Residues	8
Details	Modified residue: {"description":"Phosphotyrosine","evidences":[{"source":"PubMed","id":"22673903","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI9
Number of Residues	16
Details	Modified residue: {"description":"Phosphothreonine","evidences":[{"source":"PubMed","id":"22673903","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI10
Number of Residues	16
Details	Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternate","evidences":[{"source":"UniProtKB","id":"P00338","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI11
Number of Residues	1
Details	Modified residue: {"description":"N-acetylalanine","evidences":[{"source":"Reference","evidenceCode":"ECO:0000269","citation":{"citationType":"submission","publicationDate":"JUN-2009","submissionDatabase":"UniProtKB","authors":["Bienvenut W.V.","von Kriegsheim A.F.","Kolch W."]}}]}

Chain

Residue

Details

---