Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

5EJ7

EcMenD-ThDP-Mn2+ complex soaked with 2-ketoglutarate for 21 s

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000287	molecular_function	magnesium ion binding
A	0003824	molecular_function	catalytic activity
A	0009234	biological_process	menaquinone biosynthetic process
A	0016740	molecular_function	transferase activity
A	0030145	molecular_function	manganese ion binding
A	0030976	molecular_function	thiamine pyrophosphate binding
A	0042803	molecular_function	protein homodimerization activity
A	0046872	molecular_function	metal ion binding
A	0070204	molecular_function	2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylic-acid synthase activity
B	0000287	molecular_function	magnesium ion binding
B	0003824	molecular_function	catalytic activity
B	0009234	biological_process	menaquinone biosynthetic process
B	0016740	molecular_function	transferase activity
B	0030145	molecular_function	manganese ion binding
B	0030976	molecular_function	thiamine pyrophosphate binding
B	0042803	molecular_function	protein homodimerization activity
B	0046872	molecular_function	metal ion binding
B	0070204	molecular_function	2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylic-acid synthase activity
C	0000287	molecular_function	magnesium ion binding
C	0003824	molecular_function	catalytic activity
C	0009234	biological_process	menaquinone biosynthetic process
C	0016740	molecular_function	transferase activity
C	0030145	molecular_function	manganese ion binding
C	0030976	molecular_function	thiamine pyrophosphate binding
C	0042803	molecular_function	protein homodimerization activity
C	0046872	molecular_function	metal ion binding
C	0070204	molecular_function	2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylic-acid synthase activity
D	0000287	molecular_function	magnesium ion binding
D	0003824	molecular_function	catalytic activity
D	0009234	biological_process	menaquinone biosynthetic process
D	0016740	molecular_function	transferase activity
D	0030145	molecular_function	manganese ion binding
D	0030976	molecular_function	thiamine pyrophosphate binding
D	0042803	molecular_function	protein homodimerization activity
D	0046872	molecular_function	metal ion binding
D	0070204	molecular_function	2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylic-acid synthase activity
E	0000287	molecular_function	magnesium ion binding
E	0003824	molecular_function	catalytic activity
E	0009234	biological_process	menaquinone biosynthetic process
E	0016740	molecular_function	transferase activity
E	0030145	molecular_function	manganese ion binding
E	0030976	molecular_function	thiamine pyrophosphate binding
E	0042803	molecular_function	protein homodimerization activity
E	0046872	molecular_function	metal ion binding
E	0070204	molecular_function	2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylic-acid synthase activity
F	0000287	molecular_function	magnesium ion binding
F	0003824	molecular_function	catalytic activity
F	0009234	biological_process	menaquinone biosynthetic process
F	0016740	molecular_function	transferase activity
F	0030145	molecular_function	manganese ion binding
F	0030976	molecular_function	thiamine pyrophosphate binding
F	0042803	molecular_function	protein homodimerization activity
F	0046872	molecular_function	metal ion binding
F	0070204	molecular_function	2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylic-acid synthase activity
G	0000287	molecular_function	magnesium ion binding
G	0003824	molecular_function	catalytic activity
G	0009234	biological_process	menaquinone biosynthetic process
G	0016740	molecular_function	transferase activity
G	0030145	molecular_function	manganese ion binding
G	0030976	molecular_function	thiamine pyrophosphate binding
G	0042803	molecular_function	protein homodimerization activity
G	0046872	molecular_function	metal ion binding
G	0070204	molecular_function	2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylic-acid synthase activity
H	0000287	molecular_function	magnesium ion binding
H	0003824	molecular_function	catalytic activity
H	0009234	biological_process	menaquinone biosynthetic process
H	0016740	molecular_function	transferase activity
H	0030145	molecular_function	manganese ion binding
H	0030976	molecular_function	thiamine pyrophosphate binding
H	0042803	molecular_function	protein homodimerization activity
H	0046872	molecular_function	metal ion binding
H	0070204	molecular_function	2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylic-acid synthase activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	29
Details	binding site for residue TD6 A 601

Chain	Residue
A	ASN390
A	GLY441
A	ASP442
A	LEU443
A	SER444
A	TYR447
A	ASN469
A	GLY471
A	GLY472
A	GLN473
A	ILE474
A	SER391
A	MN602
A	HOH803
A	HOH820
A	HOH945
B	PRO30
B	GLU55
B	THR81
B	ALA82
B	ASN117
B	GLN118
A	LEU392
A	ARG395
A	ARG413
A	SER416
A	GLY417
A	ILE418
A	ASP419

site_id	AC2
Number of Residues	5
Details	binding site for residue MN A 602

Chain	Residue
A	ASP442
A	ASN469
A	GLY471
A	TD6601
A	HOH803

site_id	AC3
Number of Residues	9
Details	binding site for residue GOL A 603

Chain	Residue
A	HIS49
A	HIS50
A	THR51
A	ARG430
A	LEU454
A	GLN457
A	HOH744
A	HOH763
A	HOH1056

site_id	AC4
Number of Residues	4
Details	binding site for residue GOL A 604

Chain	Residue
A	ARG364
A	HIS374
A	ASP378
A	HOH1115

site_id	AC5
Number of Residues	28
Details	binding site for residue TD6 B 601

Chain	Residue
A	PRO30
A	GLU55
A	THR81
A	ALA82
A	ASN117
A	GLN118
B	ASN390
B	SER391
B	LEU392
B	ARG395
B	ARG413
B	SER416
B	GLY417
B	ILE418
B	ASP419
B	GLY441
B	ASP442
B	LEU443
B	SER444
B	ASN469
B	GLY471
B	GLY472
B	GLN473
B	ILE474
B	MN602
B	HOH884
B	HOH887
B	HOH890

site_id	AC6
Number of Residues	5
Details	binding site for residue MN B 602

Chain	Residue
B	ASP442
B	ASN469
B	GLY471
B	TD6601
B	HOH887

site_id	AC7
Number of Residues	8
Details	binding site for residue GOL B 603

Chain	Residue
B	HIS49
B	HIS50
B	THR51
B	ARG430
B	LEU454
B	HOH728
B	HOH732
B	HOH956

site_id	AC8
Number of Residues	30
Details	binding site for residue TD6 C 601

Chain	Residue
C	LEU443
C	SER444
C	TYR447
C	ASN469
C	GLY471
C	GLY472
C	GLN473
C	ILE474
C	MN602
C	HOH6128
C	HOH6206
C	HOH6263
C	HOH6304
D	PRO30
D	GLU55
D	THR81
D	ALA82
D	ASN117
D	GLN118
C	ASN390
C	SER391
C	LEU392
C	ARG395
C	ARG413
C	SER416
C	GLY417
C	ILE418
C	ASP419
C	GLY441
C	ASP442

site_id	AC9
Number of Residues	5
Details	binding site for residue MN C 602

Chain	Residue
C	ASP442
C	ASN469
C	GLY471
C	TD6601
C	HOH6206

site_id	AD1
Number of Residues	8
Details	binding site for residue GOL C 603

Chain	Residue
C	HIS49
C	HIS50
C	THR51
C	ARG430
C	LEU454
C	HOH6116
C	HOH6135
C	HOH6496

site_id	AD2
Number of Residues	5
Details	binding site for residue GOL C 604

Chain	Residue
B	HOH1406
C	ARG364
C	HIS374
C	ASP378
C	HOH6376

site_id	AD3
Number of Residues	30
Details	binding site for residue TD6 D 601

Chain	Residue
C	PRO30
C	GLU55
C	THR81
C	ALA82
C	ASN117
C	GLN118
C	HOH6356
D	ASN390
D	SER391
D	LEU392
D	ARG395
D	ARG413
D	SER416
D	GLY417
D	ILE418
D	ASP419
D	GLY441
D	ASP442
D	LEU443
D	SER444
D	TYR447
D	ASN469
D	GLY471
D	GLY472
D	GLN473
D	ILE474
D	MN602
D	HOH792
D	HOH860
D	HOH864

site_id	AD4
Number of Residues	5
Details	binding site for residue MN D 602

Chain	Residue
D	ASP442
D	ASN469
D	GLY471
D	TD6601
D	HOH864

site_id	AD5
Number of Residues	7
Details	binding site for residue GOL D 603

Chain	Residue
D	HIS50
D	THR51
D	ARG430
D	LEU454
D	HOH724
D	HOH760
D	HOH1022

site_id	AD6
Number of Residues	4
Details	binding site for residue GOL D 604

Chain	Residue
D	ARG364
D	HIS374
D	ASP378
D	HOH803

site_id	AD7
Number of Residues	29
Details	binding site for residue TD6 E 601

Chain	Residue
E	ASN390
E	SER391
E	LEU392
E	ARG395
E	ARG413
E	SER416
E	GLY417
E	ILE418
E	ASP419
E	GLY441
E	ASP442
E	LEU443
E	SER444
E	TYR447
E	ASN469
E	GLY471
E	GLY472
E	GLN473
E	ILE474
E	MN602
E	HOH787
E	HOH843
E	HOH906
F	PRO30
F	GLU55
F	THR81
F	ALA82
F	ASN117
F	GLN118

site_id	AD8
Number of Residues	5
Details	binding site for residue MN E 602

Chain	Residue
E	ASP442
E	ASN469
E	GLY471
E	TD6601
E	HOH843

site_id	AD9
Number of Residues	9
Details	binding site for residue GOL E 603

Chain	Residue
E	HIS49
E	HIS50
E	THR51
E	ARG430
E	LEU454
E	GLN457
E	HOH728
E	HOH784
E	HOH934

site_id	AE1
Number of Residues	4
Details	binding site for residue GOL E 604

Chain	Residue
E	ARG364
E	HIS374
E	ARG375
E	ASP378

site_id	AE2
Number of Residues	29
Details	binding site for residue TD6 F 601

Chain	Residue
E	PRO30
E	GLU55
E	THR81
E	ALA82
E	ASN117
E	GLN118
F	ASN390
F	SER391
F	LEU392
F	ARG395
F	ARG413
F	SER416
F	GLY417
F	ILE418
F	ASP419
F	GLY441
F	ASP442
F	LEU443
F	SER444
F	TYR447
F	ASN469
F	GLY471
F	GLY472
F	GLN473
F	ILE474
F	MN602
F	HOH744
F	HOH830
F	HOH963

site_id	AE3
Number of Residues	5
Details	binding site for residue MN F 602

Chain	Residue
F	ASP442
F	ASN469
F	GLY471
F	TD6601
F	HOH744

site_id	AE4
Number of Residues	7
Details	binding site for residue GOL F 603

Chain	Residue
F	HIS50
F	THR51
F	ARG430
F	LEU454
F	HOH718
F	HOH724
F	HOH996

site_id	AE5
Number of Residues	28
Details	binding site for residue TD6 G 601

Chain	Residue
G	ASN390
G	SER391
G	LEU392
G	ARG395
G	ARG413
G	SER416
G	GLY417
G	ILE418
G	ASP419
G	GLY441
G	ASP442
G	LEU443
G	SER444
G	ASN469
G	GLY471
G	GLY472
G	GLN473
G	ILE474
G	MN602
G	HOH789
G	HOH832
G	HOH847
G	HOH1090
H	PRO30
H	GLU55
H	THR81
H	ASN117
H	GLN118

site_id	AE6
Number of Residues	5
Details	binding site for residue MN G 602

Chain	Residue
G	ASP442
G	ASN469
G	GLY471
G	TD6601
G	HOH832

site_id	AE7
Number of Residues	8
Details	binding site for residue GOL G 603

Chain	Residue
G	HIS49
G	HIS50
G	THR51
G	ARG430
G	LEU454
G	GLN457
G	HOH746
G	HOH1021

site_id	AE8
Number of Residues	5
Details	binding site for residue GOL G 604

Chain	Residue
G	ARG364
G	HIS374
G	ARG375
G	ASP378
G	HOH964

site_id	AE9
Number of Residues	6
Details	binding site for residue MN G 605

Chain	Residue
F	GLU16
F	HOH841
F	HOH896
F	HOH999
F	HOH1203
G	GLU354

site_id	AF1
Number of Residues	28
Details	binding site for residue TD6 H 601

Chain	Residue
G	PRO30
G	GLU55
G	THR81
G	ALA82
G	ASN117
G	GLN118
H	ASN390
H	SER391
H	LEU392
H	ARG395
H	ARG413
H	SER416
H	GLY417
H	ILE418
H	ASP419
H	GLY441
H	ASP442
H	LEU443
H	SER444
H	ASN469
H	GLY471
H	GLY472
H	GLN473
H	ILE474
H	MN602
H	HOH815
H	HOH826
H	HOH963

site_id	AF2
Number of Residues	5
Details	binding site for residue MN H 602

Chain	Residue
H	ASP442
H	ASN469
H	GLY471
H	TD6601
H	HOH815

site_id	AF3
Number of Residues	9
Details	binding site for residue GOL H 603

Chain	Residue
H	HIS49
H	HIS50
H	THR51
H	ARG430
H	LEU454
H	GLN457
H	HOH715
H	HOH794
H	HOH845

site_id	AF4
Number of Residues	5
Details	binding site for residue GOL H 604

Chain	Residue
H	ARG364
H	HIS374
H	ARG375
H	ASP378
H	HOH925

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)