5EC5
Crystal structure of lysenin pore
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0005576 | cellular_component | extracellular region |
| A | 0006811 | biological_process | monoatomic ion transport |
| A | 0016020 | cellular_component | membrane |
| A | 0031640 | biological_process | killing of cells of another organism |
| A | 0035821 | biological_process | modulation of process of another organism |
| A | 0042742 | biological_process | defense response to bacterium |
| A | 0044218 | cellular_component | other organism cell membrane |
| A | 0090729 | molecular_function | toxin activity |
| B | 0005576 | cellular_component | extracellular region |
| B | 0006811 | biological_process | monoatomic ion transport |
| B | 0016020 | cellular_component | membrane |
| B | 0031640 | biological_process | killing of cells of another organism |
| B | 0035821 | biological_process | modulation of process of another organism |
| B | 0042742 | biological_process | defense response to bacterium |
| B | 0044218 | cellular_component | other organism cell membrane |
| B | 0090729 | molecular_function | toxin activity |
| C | 0005576 | cellular_component | extracellular region |
| C | 0006811 | biological_process | monoatomic ion transport |
| C | 0016020 | cellular_component | membrane |
| C | 0031640 | biological_process | killing of cells of another organism |
| C | 0035821 | biological_process | modulation of process of another organism |
| C | 0042742 | biological_process | defense response to bacterium |
| C | 0044218 | cellular_component | other organism cell membrane |
| C | 0090729 | molecular_function | toxin activity |
| D | 0005576 | cellular_component | extracellular region |
| D | 0006811 | biological_process | monoatomic ion transport |
| D | 0016020 | cellular_component | membrane |
| D | 0031640 | biological_process | killing of cells of another organism |
| D | 0035821 | biological_process | modulation of process of another organism |
| D | 0042742 | biological_process | defense response to bacterium |
| D | 0044218 | cellular_component | other organism cell membrane |
| D | 0090729 | molecular_function | toxin activity |
| E | 0005576 | cellular_component | extracellular region |
| E | 0006811 | biological_process | monoatomic ion transport |
| E | 0016020 | cellular_component | membrane |
| E | 0031640 | biological_process | killing of cells of another organism |
| E | 0035821 | biological_process | modulation of process of another organism |
| E | 0042742 | biological_process | defense response to bacterium |
| E | 0044218 | cellular_component | other organism cell membrane |
| E | 0090729 | molecular_function | toxin activity |
| F | 0005576 | cellular_component | extracellular region |
| F | 0006811 | biological_process | monoatomic ion transport |
| F | 0016020 | cellular_component | membrane |
| F | 0031640 | biological_process | killing of cells of another organism |
| F | 0035821 | biological_process | modulation of process of another organism |
| F | 0042742 | biological_process | defense response to bacterium |
| F | 0044218 | cellular_component | other organism cell membrane |
| F | 0090729 | molecular_function | toxin activity |
| G | 0005576 | cellular_component | extracellular region |
| G | 0006811 | biological_process | monoatomic ion transport |
| G | 0016020 | cellular_component | membrane |
| G | 0031640 | biological_process | killing of cells of another organism |
| G | 0035821 | biological_process | modulation of process of another organism |
| G | 0042742 | biological_process | defense response to bacterium |
| G | 0044218 | cellular_component | other organism cell membrane |
| G | 0090729 | molecular_function | toxin activity |
| H | 0005576 | cellular_component | extracellular region |
| H | 0006811 | biological_process | monoatomic ion transport |
| H | 0016020 | cellular_component | membrane |
| H | 0031640 | biological_process | killing of cells of another organism |
| H | 0035821 | biological_process | modulation of process of another organism |
| H | 0042742 | biological_process | defense response to bacterium |
| H | 0044218 | cellular_component | other organism cell membrane |
| H | 0090729 | molecular_function | toxin activity |
| I | 0005576 | cellular_component | extracellular region |
| I | 0006811 | biological_process | monoatomic ion transport |
| I | 0016020 | cellular_component | membrane |
| I | 0031640 | biological_process | killing of cells of another organism |
| I | 0035821 | biological_process | modulation of process of another organism |
| I | 0042742 | biological_process | defense response to bacterium |
| I | 0044218 | cellular_component | other organism cell membrane |
| I | 0090729 | molecular_function | toxin activity |
| J | 0005576 | cellular_component | extracellular region |
| J | 0006811 | biological_process | monoatomic ion transport |
| J | 0016020 | cellular_component | membrane |
| J | 0031640 | biological_process | killing of cells of another organism |
| J | 0035821 | biological_process | modulation of process of another organism |
| J | 0042742 | biological_process | defense response to bacterium |
| J | 0044218 | cellular_component | other organism cell membrane |
| J | 0090729 | molecular_function | toxin activity |
| K | 0005576 | cellular_component | extracellular region |
| K | 0006811 | biological_process | monoatomic ion transport |
| K | 0016020 | cellular_component | membrane |
| K | 0031640 | biological_process | killing of cells of another organism |
| K | 0035821 | biological_process | modulation of process of another organism |
| K | 0042742 | biological_process | defense response to bacterium |
| K | 0044218 | cellular_component | other organism cell membrane |
| K | 0090729 | molecular_function | toxin activity |
| L | 0005576 | cellular_component | extracellular region |
| L | 0006811 | biological_process | monoatomic ion transport |
| L | 0016020 | cellular_component | membrane |
| L | 0031640 | biological_process | killing of cells of another organism |
| L | 0035821 | biological_process | modulation of process of another organism |
| L | 0042742 | biological_process | defense response to bacterium |
| L | 0044218 | cellular_component | other organism cell membrane |
| L | 0090729 | molecular_function | toxin activity |
| M | 0005576 | cellular_component | extracellular region |
| M | 0006811 | biological_process | monoatomic ion transport |
| M | 0016020 | cellular_component | membrane |
| M | 0031640 | biological_process | killing of cells of another organism |
| M | 0035821 | biological_process | modulation of process of another organism |
| M | 0042742 | biological_process | defense response to bacterium |
| M | 0044218 | cellular_component | other organism cell membrane |
| M | 0090729 | molecular_function | toxin activity |
| N | 0005576 | cellular_component | extracellular region |
| N | 0006811 | biological_process | monoatomic ion transport |
| N | 0016020 | cellular_component | membrane |
| N | 0031640 | biological_process | killing of cells of another organism |
| N | 0035821 | biological_process | modulation of process of another organism |
| N | 0042742 | biological_process | defense response to bacterium |
| N | 0044218 | cellular_component | other organism cell membrane |
| N | 0090729 | molecular_function | toxin activity |
| O | 0005576 | cellular_component | extracellular region |
| O | 0006811 | biological_process | monoatomic ion transport |
| O | 0016020 | cellular_component | membrane |
| O | 0031640 | biological_process | killing of cells of another organism |
| O | 0035821 | biological_process | modulation of process of another organism |
| O | 0042742 | biological_process | defense response to bacterium |
| O | 0044218 | cellular_component | other organism cell membrane |
| O | 0090729 | molecular_function | toxin activity |
| P | 0005576 | cellular_component | extracellular region |
| P | 0006811 | biological_process | monoatomic ion transport |
| P | 0016020 | cellular_component | membrane |
| P | 0031640 | biological_process | killing of cells of another organism |
| P | 0035821 | biological_process | modulation of process of another organism |
| P | 0042742 | biological_process | defense response to bacterium |
| P | 0044218 | cellular_component | other organism cell membrane |
| P | 0090729 | molecular_function | toxin activity |
| R | 0005576 | cellular_component | extracellular region |
| R | 0006811 | biological_process | monoatomic ion transport |
| R | 0016020 | cellular_component | membrane |
| R | 0031640 | biological_process | killing of cells of another organism |
| R | 0035821 | biological_process | modulation of process of another organism |
| R | 0042742 | biological_process | defense response to bacterium |
| R | 0044218 | cellular_component | other organism cell membrane |
| R | 0090729 | molecular_function | toxin activity |
| S | 0005576 | cellular_component | extracellular region |
| S | 0006811 | biological_process | monoatomic ion transport |
| S | 0016020 | cellular_component | membrane |
| S | 0031640 | biological_process | killing of cells of another organism |
| S | 0035821 | biological_process | modulation of process of another organism |
| S | 0042742 | biological_process | defense response to bacterium |
| S | 0044218 | cellular_component | other organism cell membrane |
| S | 0090729 | molecular_function | toxin activity |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 6 |
| Details | binding site for residue MBO A 301 |
| Chain | Residue |
| A | SER226 |
| A | ASP228 |
| A | PHE266 |
| A | SER269 |
| A | CYS283 |
| A | ASP285 |
| site_id | AC2 |
| Number of Residues | 6 |
| Details | binding site for residue MBO A 302 |
| Chain | Residue |
| A | LEU284 |
| A | ARG287 |
| B | ARG52 |
| A | CYS272 |
| A | TYR273 |
| A | ASP274 |
| site_id | AC3 |
| Number of Residues | 4 |
| Details | binding site for residue MBO B 301 |
| Chain | Residue |
| B | CYS272 |
| B | TYR273 |
| B | ASP274 |
| B | ARG287 |
| site_id | AC4 |
| Number of Residues | 6 |
| Details | binding site for residue MBO B 302 |
| Chain | Residue |
| B | SER226 |
| B | ASP228 |
| B | PHE266 |
| B | SER269 |
| B | CYS283 |
| B | ASP285 |
| site_id | AC5 |
| Number of Residues | 6 |
| Details | binding site for residue MBO C 301 |
| Chain | Residue |
| C | SER226 |
| C | ASP228 |
| C | PHE266 |
| C | SER269 |
| C | CYS283 |
| C | ASP285 |
| site_id | AC6 |
| Number of Residues | 3 |
| Details | binding site for residue HG C 302 |
| Chain | Residue |
| C | CYS272 |
| C | TYR273 |
| C | ARG287 |
| site_id | AC7 |
| Number of Residues | 6 |
| Details | binding site for residue MBO D 301 |
| Chain | Residue |
| D | SER226 |
| D | ASP228 |
| D | PHE266 |
| D | SER269 |
| D | CYS283 |
| D | ASP285 |
| site_id | AC8 |
| Number of Residues | 3 |
| Details | binding site for residue HG D 302 |
| Chain | Residue |
| D | CYS272 |
| D | TYR273 |
| D | ARG287 |
| site_id | AC9 |
| Number of Residues | 6 |
| Details | binding site for residue MBO E 301 |
| Chain | Residue |
| E | SER226 |
| E | ASP228 |
| E | PHE266 |
| E | SER269 |
| E | CYS283 |
| E | ASP285 |
| site_id | AD1 |
| Number of Residues | 5 |
| Details | binding site for residue HG E 302 |
| Chain | Residue |
| E | CYS272 |
| E | TYR273 |
| E | ASP274 |
| E | LEU284 |
| E | ARG287 |
| site_id | AD2 |
| Number of Residues | 5 |
| Details | binding site for residue HG F 301 |
| Chain | Residue |
| F | CYS272 |
| F | TYR273 |
| F | ASP274 |
| F | LEU284 |
| F | ARG287 |
| site_id | AD3 |
| Number of Residues | 7 |
| Details | binding site for residue MBO F 302 |
| Chain | Residue |
| F | SER226 |
| F | ASP228 |
| F | GLN229 |
| F | GLY230 |
| F | PHE266 |
| F | SER269 |
| F | CYS283 |
| site_id | AD4 |
| Number of Residues | 8 |
| Details | binding site for residue MBO G 301 |
| Chain | Residue |
| G | SER226 |
| G | SER227 |
| G | ASP228 |
| G | GLY230 |
| G | PHE266 |
| G | SER269 |
| G | CYS283 |
| G | ASP285 |
| site_id | AD5 |
| Number of Residues | 3 |
| Details | binding site for residue HG G 302 |
| Chain | Residue |
| G | CYS272 |
| G | TYR273 |
| G | LEU284 |
| site_id | AD6 |
| Number of Residues | 5 |
| Details | binding site for residue MBO H 301 |
| Chain | Residue |
| H | CYS272 |
| H | TYR273 |
| H | ASP274 |
| H | LEU284 |
| H | ARG287 |
| site_id | AD7 |
| Number of Residues | 4 |
| Details | binding site for residue MBO H 302 |
| Chain | Residue |
| H | SER226 |
| H | PHE266 |
| H | SER269 |
| H | CYS283 |
| site_id | AD8 |
| Number of Residues | 5 |
| Details | binding site for residue HG I 301 |
| Chain | Residue |
| I | CYS272 |
| I | TYR273 |
| I | ASP274 |
| I | LEU284 |
| I | ARG287 |
| site_id | AD9 |
| Number of Residues | 6 |
| Details | binding site for residue MBO I 302 |
| Chain | Residue |
| I | SER226 |
| I | SER227 |
| I | ASP228 |
| I | PHE266 |
| I | SER269 |
| I | CYS283 |
| site_id | AE1 |
| Number of Residues | 3 |
| Details | binding site for residue HG J 301 |
| Chain | Residue |
| J | SER226 |
| J | SER269 |
| J | CYS283 |
| site_id | AE2 |
| Number of Residues | 4 |
| Details | binding site for residue HG J 302 |
| Chain | Residue |
| J | ASP274 |
| J | ARG287 |
| J | CYS272 |
| J | TYR273 |
| site_id | AE3 |
| Number of Residues | 8 |
| Details | binding site for residue MBO K 301 |
| Chain | Residue |
| K | SER226 |
| K | SER227 |
| K | ASP228 |
| K | PHE266 |
| K | SER269 |
| K | CYS283 |
| K | ASP285 |
| K | HOH406 |
| site_id | AE4 |
| Number of Residues | 5 |
| Details | binding site for residue MBO K 302 |
| Chain | Residue |
| K | CYS272 |
| K | TYR273 |
| K | ASP274 |
| K | LEU284 |
| K | ARG287 |
| site_id | AE5 |
| Number of Residues | 6 |
| Details | binding site for residue MBO L 301 |
| Chain | Residue |
| L | SER226 |
| L | ASP228 |
| L | PHE266 |
| L | SER269 |
| L | CYS283 |
| L | ASP285 |
| site_id | AE6 |
| Number of Residues | 4 |
| Details | binding site for residue MBO L 302 |
| Chain | Residue |
| L | CYS272 |
| L | TYR273 |
| L | ASP274 |
| L | ARG287 |
| site_id | AE7 |
| Number of Residues | 6 |
| Details | binding site for residue MBO M 301 |
| Chain | Residue |
| M | SER226 |
| M | ASP228 |
| M | GLY230 |
| M | PHE266 |
| M | SER269 |
| M | CYS283 |
| site_id | AE8 |
| Number of Residues | 6 |
| Details | binding site for residue MBO M 302 |
| Chain | Residue |
| L | ARG52 |
| M | CYS272 |
| M | TYR273 |
| M | ASP274 |
| M | LEU284 |
| M | ARG287 |
| site_id | AE9 |
| Number of Residues | 3 |
| Details | binding site for residue HG N 301 |
| Chain | Residue |
| N | SER226 |
| N | SER269 |
| N | CYS283 |
| site_id | AF1 |
| Number of Residues | 5 |
| Details | binding site for residue HG N 302 |
| Chain | Residue |
| N | CYS272 |
| N | TYR273 |
| N | ASP274 |
| N | LEU284 |
| N | ARG287 |
| site_id | AF2 |
| Number of Residues | 5 |
| Details | binding site for residue MBO O 301 |
| Chain | Residue |
| O | SER226 |
| O | PHE266 |
| O | SER269 |
| O | CYS283 |
| O | ASP285 |
| site_id | AF3 |
| Number of Residues | 2 |
| Details | binding site for residue HG O 302 |
| Chain | Residue |
| O | CYS272 |
| O | TYR273 |
| site_id | AF4 |
| Number of Residues | 2 |
| Details | binding site for residue HG P 301 |
| Chain | Residue |
| P | CYS272 |
| P | TYR273 |
| site_id | AF5 |
| Number of Residues | 6 |
| Details | binding site for residue MBO P 302 |
| Chain | Residue |
| P | SER226 |
| P | ASP228 |
| P | GLY230 |
| P | PHE266 |
| P | SER269 |
| P | CYS283 |
| site_id | AF6 |
| Number of Residues | 7 |
| Details | binding site for residue MBO R 301 |
| Chain | Residue |
| R | SER226 |
| R | SER227 |
| R | ASP228 |
| R | GLY230 |
| R | PHE266 |
| R | SER269 |
| R | CYS283 |
| site_id | AF7 |
| Number of Residues | 3 |
| Details | binding site for residue HG R 302 |
| Chain | Residue |
| R | CYS272 |
| R | TYR273 |
| R | ARG287 |
| site_id | AF8 |
| Number of Residues | 6 |
| Details | binding site for residue MBO S 301 |
| Chain | Residue |
| S | SER226 |
| S | ASP228 |
| S | PHE266 |
| S | SER269 |
| S | CYS283 |
| S | ASP285 |
| site_id | AF9 |
| Number of Residues | 5 |
| Details | binding site for residue HG S 302 |
| Chain | Residue |
| S | CYS272 |
| S | TYR273 |
| S | ASP274 |
| S | LEU284 |
| S | ARG287 |
Functional Information from PROSITE/UniProt
| site_id | PS00435 |
| Number of Residues | 11 |
| Details | PEROXIDASE_1 Peroxidases proximal heme-ligand signature. ETRTVTATHSI |
| Chain | Residue | Details |
| A | GLU50-ILE60 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 72 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:22819216 |
| Chain | Residue | Details |
| A | LYS185 | |
| A | SER227 | |
| A | TYR233 | |
| A | TYR282 | |
| B | LYS185 | |
| B | SER227 | |
| B | TYR233 | |
| B | TYR282 | |
| C | LYS185 | |
| C | SER227 | |
| C | TYR233 | |
| C | TYR282 | |
| D | LYS185 | |
| D | SER227 | |
| D | TYR233 | |
| D | TYR282 | |
| E | LYS185 | |
| E | SER227 | |
| E | TYR233 | |
| E | TYR282 | |
| F | LYS185 | |
| F | SER227 | |
| F | TYR233 | |
| F | TYR282 | |
| G | LYS185 | |
| G | SER227 | |
| G | TYR233 | |
| G | TYR282 | |
| H | LYS185 | |
| H | SER227 | |
| H | TYR233 | |
| H | TYR282 | |
| I | LYS185 | |
| I | SER227 | |
| I | TYR233 | |
| I | TYR282 | |
| J | LYS185 | |
| J | SER227 | |
| J | TYR233 | |
| J | TYR282 | |
| K | LYS185 | |
| K | SER227 | |
| K | TYR233 | |
| K | TYR282 | |
| L | LYS185 | |
| L | SER227 | |
| L | TYR233 | |
| L | TYR282 | |
| M | LYS185 | |
| M | SER227 | |
| M | TYR233 | |
| M | TYR282 | |
| N | LYS185 | |
| N | SER227 | |
| N | TYR233 | |
| N | TYR282 | |
| O | LYS185 | |
| O | SER227 | |
| O | TYR233 | |
| O | TYR282 | |
| P | LYS185 | |
| P | SER227 | |
| P | TYR233 | |
| P | TYR282 | |
| R | LYS185 | |
| R | SER227 | |
| R | TYR233 | |
| R | TYR282 | |
| S | LYS185 | |
| S | SER227 | |
| S | TYR233 | |
| S | TYR282 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 54 |
| Details | SITE: Crucial for binding sphingomyelin and inducing hemolysis => ECO:0000269|PubMed:15274631 |
| Chain | Residue | Details |
| A | TRP20 | |
| A | TRP245 | |
| A | TRP291 | |
| B | TRP20 | |
| B | TRP245 | |
| B | TRP291 | |
| C | TRP20 | |
| C | TRP245 | |
| C | TRP291 | |
| D | TRP20 | |
| D | TRP245 | |
| D | TRP291 | |
| E | TRP20 | |
| E | TRP245 | |
| E | TRP291 | |
| F | TRP20 | |
| F | TRP245 | |
| F | TRP291 | |
| G | TRP20 | |
| G | TRP245 | |
| G | TRP291 | |
| H | TRP20 | |
| H | TRP245 | |
| H | TRP291 | |
| I | TRP20 | |
| I | TRP245 | |
| I | TRP291 | |
| J | TRP20 | |
| J | TRP245 | |
| J | TRP291 | |
| K | TRP20 | |
| K | TRP245 | |
| K | TRP291 | |
| L | TRP20 | |
| L | TRP245 | |
| L | TRP291 | |
| M | TRP20 | |
| M | TRP245 | |
| M | TRP291 | |
| N | TRP20 | |
| N | TRP245 | |
| N | TRP291 | |
| O | TRP20 | |
| O | TRP245 | |
| O | TRP291 | |
| P | TRP20 | |
| P | TRP245 | |
| P | TRP291 | |
| R | TRP20 | |
| R | TRP245 | |
| R | TRP291 | |
| S | TRP20 | |
| S | TRP245 | |
| S | TRP291 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 18 |
| Details | SITE: Crucial for binding sphingomyelin and important for inducing hemolysis => ECO:0000269|PubMed:15274631 |
| Chain | Residue | Details |
| A | TRP187 | |
| B | TRP187 | |
| C | TRP187 | |
| D | TRP187 | |
| E | TRP187 | |
| F | TRP187 | |
| G | TRP187 | |
| H | TRP187 | |
| I | TRP187 | |
| J | TRP187 | |
| K | TRP187 | |
| L | TRP187 | |
| M | TRP187 | |
| N | TRP187 | |
| O | TRP187 | |
| P | TRP187 | |
| R | TRP187 | |
| S | TRP187 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 18 |
| Details | SITE: Important for activity |
| Chain | Residue | Details |
| A | PHE209 | |
| B | PHE209 | |
| C | PHE209 | |
| D | PHE209 | |
| E | PHE209 | |
| F | PHE209 | |
| G | PHE209 | |
| H | PHE209 | |
| I | PHE209 | |
| J | PHE209 | |
| K | PHE209 | |
| L | PHE209 | |
| M | PHE209 | |
| N | PHE209 | |
| O | PHE209 | |
| P | PHE209 | |
| R | PHE209 | |
| S | PHE209 |
