5EC5
Crystal structure of lysenin pore
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-03-10 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 192.994, 192.994, 493.202 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.100 - 3.100 |
| R-factor | 0.21658 |
| Rwork | 0.214 |
| R-free | 0.25787 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.474 |
| Data reduction software | XDS (VERSION November 3, 2014) |
| Data scaling software | XDS (VERSION November 3, 2014) |
| Phasing software | PHENIX (1.9-1692) |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.290 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Number of reflections | 160505 | |
| <I/σ(I)> | 8.9 | 2.3 |
| Completeness [%] | 99.9 | 99.7 |
| Redundancy | 8.1 | 8.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | PEG 400, magnesium chloride, Hepes |
