5DJ4

Leucine-bound Sestrin2 from Homo sapiens

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0001932	biological_process	regulation of protein phosphorylation
A	0004601	molecular_function	peroxidase activity
A	0005092	molecular_function	GDP-dissociation inhibitor activity
A	0005515	molecular_function	protein binding
A	0005634	cellular_component	nucleus
A	0005737	cellular_component	cytoplasm
A	0005765	cellular_component	lysosomal membrane
A	0005829	cellular_component	cytosol
A	0016239	biological_process	positive regulation of macroautophagy
A	0016491	molecular_function	oxidoreductase activity
A	0016684	molecular_function	oxidoreductase activity, acting on peroxide as acceptor
A	0030308	biological_process	negative regulation of cell growth
A	0030330	biological_process	DNA damage response, signal transduction by p53 class mediator
A	0031588	cellular_component	nucleotide-activated protein kinase complex
A	0031932	cellular_component	TORC2 complex
A	0032147	biological_process	activation of protein kinase activity
A	0032542	molecular_function	sulfiredoxin activity
A	0034198	biological_process	cellular response to amino acid starvation
A	0034599	biological_process	cellular response to oxidative stress
A	0038203	biological_process	TORC2 signaling
A	0042149	biological_process	cellular response to glucose starvation
A	0042731	molecular_function	PH domain binding
A	0044877	molecular_function	protein-containing complex binding
A	0061700	cellular_component	GATOR2 complex
A	0070728	molecular_function	L-leucine binding
A	0071230	biological_process	cellular response to amino acid stimulus
A	0071233	biological_process	cellular response to L-leucine
A	0072593	biological_process	reactive oxygen species metabolic process
A	0072659	biological_process	protein localization to plasma membrane
A	0098869	biological_process	cellular oxidant detoxification
A	0140311	molecular_function	protein sequestering activity
A	1900182	biological_process	positive regulation of protein localization to nucleus
A	1901031	biological_process	regulation of response to reactive oxygen species
A	1902010	biological_process	negative regulation of translation in response to endoplasmic reticulum stress
A	1903432	biological_process	regulation of TORC1 signaling
A	1904262	biological_process	negative regulation of TORC1 signaling
A	1904263	biological_process	positive regulation of TORC1 signaling
A	1990253	biological_process	cellular response to leucine starvation
A	1990316	cellular_component	Atg1/ULK1 kinase complex
B	0001932	biological_process	regulation of protein phosphorylation
B	0004601	molecular_function	peroxidase activity
B	0005092	molecular_function	GDP-dissociation inhibitor activity
B	0005515	molecular_function	protein binding
B	0005634	cellular_component	nucleus
B	0005737	cellular_component	cytoplasm
B	0005765	cellular_component	lysosomal membrane
B	0005829	cellular_component	cytosol
B	0016239	biological_process	positive regulation of macroautophagy
B	0016491	molecular_function	oxidoreductase activity
B	0016684	molecular_function	oxidoreductase activity, acting on peroxide as acceptor
B	0030308	biological_process	negative regulation of cell growth
B	0030330	biological_process	DNA damage response, signal transduction by p53 class mediator
B	0031588	cellular_component	nucleotide-activated protein kinase complex
B	0031932	cellular_component	TORC2 complex
B	0032147	biological_process	activation of protein kinase activity
B	0032542	molecular_function	sulfiredoxin activity
B	0034198	biological_process	cellular response to amino acid starvation
B	0034599	biological_process	cellular response to oxidative stress
B	0038203	biological_process	TORC2 signaling
B	0042149	biological_process	cellular response to glucose starvation
B	0042731	molecular_function	PH domain binding
B	0044877	molecular_function	protein-containing complex binding
B	0061700	cellular_component	GATOR2 complex
B	0070728	molecular_function	L-leucine binding
B	0071230	biological_process	cellular response to amino acid stimulus
B	0071233	biological_process	cellular response to L-leucine
B	0072593	biological_process	reactive oxygen species metabolic process
B	0072659	biological_process	protein localization to plasma membrane
B	0098869	biological_process	cellular oxidant detoxification
B	0140311	molecular_function	protein sequestering activity
B	1900182	biological_process	positive regulation of protein localization to nucleus
B	1901031	biological_process	regulation of response to reactive oxygen species
B	1902010	biological_process	negative regulation of translation in response to endoplasmic reticulum stress
B	1903432	biological_process	regulation of TORC1 signaling
B	1904262	biological_process	negative regulation of TORC1 signaling
B	1904263	biological_process	positive regulation of TORC1 signaling
B	1990253	biological_process	cellular response to leucine starvation
B	1990316	cellular_component	Atg1/ULK1 kinase complex
C	0001932	biological_process	regulation of protein phosphorylation
C	0004601	molecular_function	peroxidase activity
C	0005092	molecular_function	GDP-dissociation inhibitor activity
C	0005515	molecular_function	protein binding
C	0005634	cellular_component	nucleus
C	0005737	cellular_component	cytoplasm
C	0005765	cellular_component	lysosomal membrane
C	0005829	cellular_component	cytosol
C	0016239	biological_process	positive regulation of macroautophagy
C	0016491	molecular_function	oxidoreductase activity
C	0016684	molecular_function	oxidoreductase activity, acting on peroxide as acceptor
C	0030308	biological_process	negative regulation of cell growth
C	0030330	biological_process	DNA damage response, signal transduction by p53 class mediator
C	0031588	cellular_component	nucleotide-activated protein kinase complex
C	0031932	cellular_component	TORC2 complex
C	0032147	biological_process	activation of protein kinase activity
C	0032542	molecular_function	sulfiredoxin activity
C	0034198	biological_process	cellular response to amino acid starvation
C	0034599	biological_process	cellular response to oxidative stress
C	0038203	biological_process	TORC2 signaling
C	0042149	biological_process	cellular response to glucose starvation
C	0042731	molecular_function	PH domain binding
C	0044877	molecular_function	protein-containing complex binding
C	0061700	cellular_component	GATOR2 complex
C	0070728	molecular_function	L-leucine binding
C	0071230	biological_process	cellular response to amino acid stimulus
C	0071233	biological_process	cellular response to L-leucine
C	0072593	biological_process	reactive oxygen species metabolic process
C	0072659	biological_process	protein localization to plasma membrane
C	0098869	biological_process	cellular oxidant detoxification
C	0140311	molecular_function	protein sequestering activity
C	1900182	biological_process	positive regulation of protein localization to nucleus
C	1901031	biological_process	regulation of response to reactive oxygen species
C	1902010	biological_process	negative regulation of translation in response to endoplasmic reticulum stress
C	1903432	biological_process	regulation of TORC1 signaling
C	1904262	biological_process	negative regulation of TORC1 signaling
C	1904263	biological_process	positive regulation of TORC1 signaling
C	1990253	biological_process	cellular response to leucine starvation
C	1990316	cellular_component	Atg1/ULK1 kinase complex
D	0001932	biological_process	regulation of protein phosphorylation
D	0004601	molecular_function	peroxidase activity
D	0005092	molecular_function	GDP-dissociation inhibitor activity
D	0005515	molecular_function	protein binding
D	0005634	cellular_component	nucleus
D	0005737	cellular_component	cytoplasm
D	0005765	cellular_component	lysosomal membrane
D	0005829	cellular_component	cytosol
D	0016239	biological_process	positive regulation of macroautophagy
D	0016491	molecular_function	oxidoreductase activity
D	0016684	molecular_function	oxidoreductase activity, acting on peroxide as acceptor
D	0030308	biological_process	negative regulation of cell growth
D	0030330	biological_process	DNA damage response, signal transduction by p53 class mediator
D	0031588	cellular_component	nucleotide-activated protein kinase complex
D	0031932	cellular_component	TORC2 complex
D	0032147	biological_process	activation of protein kinase activity
D	0032542	molecular_function	sulfiredoxin activity
D	0034198	biological_process	cellular response to amino acid starvation
D	0034599	biological_process	cellular response to oxidative stress
D	0038203	biological_process	TORC2 signaling
D	0042149	biological_process	cellular response to glucose starvation
D	0042731	molecular_function	PH domain binding
D	0044877	molecular_function	protein-containing complex binding
D	0061700	cellular_component	GATOR2 complex
D	0070728	molecular_function	L-leucine binding
D	0071230	biological_process	cellular response to amino acid stimulus
D	0071233	biological_process	cellular response to L-leucine
D	0072593	biological_process	reactive oxygen species metabolic process
D	0072659	biological_process	protein localization to plasma membrane
D	0098869	biological_process	cellular oxidant detoxification
D	0140311	molecular_function	protein sequestering activity
D	1900182	biological_process	positive regulation of protein localization to nucleus
D	1901031	biological_process	regulation of response to reactive oxygen species
D	1902010	biological_process	negative regulation of translation in response to endoplasmic reticulum stress
D	1903432	biological_process	regulation of TORC1 signaling
D	1904262	biological_process	negative regulation of TORC1 signaling
D	1904263	biological_process	positive regulation of TORC1 signaling
D	1990253	biological_process	cellular response to leucine starvation
D	1990316	cellular_component	Atg1/ULK1 kinase complex
E	0001932	biological_process	regulation of protein phosphorylation
E	0004601	molecular_function	peroxidase activity
E	0005092	molecular_function	GDP-dissociation inhibitor activity
E	0005515	molecular_function	protein binding
E	0005634	cellular_component	nucleus
E	0005737	cellular_component	cytoplasm
E	0005765	cellular_component	lysosomal membrane
E	0005829	cellular_component	cytosol
E	0016239	biological_process	positive regulation of macroautophagy
E	0016491	molecular_function	oxidoreductase activity
E	0016684	molecular_function	oxidoreductase activity, acting on peroxide as acceptor
E	0030308	biological_process	negative regulation of cell growth
E	0030330	biological_process	DNA damage response, signal transduction by p53 class mediator
E	0031588	cellular_component	nucleotide-activated protein kinase complex
E	0031932	cellular_component	TORC2 complex
E	0032147	biological_process	activation of protein kinase activity
E	0032542	molecular_function	sulfiredoxin activity
E	0034198	biological_process	cellular response to amino acid starvation
E	0034599	biological_process	cellular response to oxidative stress
E	0038203	biological_process	TORC2 signaling
E	0042149	biological_process	cellular response to glucose starvation
E	0042731	molecular_function	PH domain binding
E	0044877	molecular_function	protein-containing complex binding
E	0061700	cellular_component	GATOR2 complex
E	0070728	molecular_function	L-leucine binding
E	0071230	biological_process	cellular response to amino acid stimulus
E	0071233	biological_process	cellular response to L-leucine
E	0072593	biological_process	reactive oxygen species metabolic process
E	0072659	biological_process	protein localization to plasma membrane
E	0098869	biological_process	cellular oxidant detoxification
E	0140311	molecular_function	protein sequestering activity
E	1900182	biological_process	positive regulation of protein localization to nucleus
E	1901031	biological_process	regulation of response to reactive oxygen species
E	1902010	biological_process	negative regulation of translation in response to endoplasmic reticulum stress
E	1903432	biological_process	regulation of TORC1 signaling
E	1904262	biological_process	negative regulation of TORC1 signaling
E	1904263	biological_process	positive regulation of TORC1 signaling
E	1990253	biological_process	cellular response to leucine starvation
E	1990316	cellular_component	Atg1/ULK1 kinase complex

Functional Information from PDB Data

site_id	AC1
Number of Residues	9
Details	binding site for residue LEU A 501

Chain	Residue
A	THR374
A	TYR375
A	ASN376
A	THR377
A	THR386
A	ARG390
A	GLU451
A	HIS454
A	HOH615

---

site_id	AC2
Number of Residues	9
Details	binding site for residue LEU B 501

Chain	Residue
B	THR374
B	TYR375
B	ASN376
B	THR377
B	THR386
B	ARG390
B	GLU451
B	HIS454
B	HOH614

---

site_id	AC3
Number of Residues	7
Details	binding site for residue LEU C 501

Chain	Residue
C	THR374
C	TYR375
C	ASN376
C	THR377
C	THR386
C	GLU451
C	HIS454

---

site_id	AC4
Number of Residues	8
Details	binding site for residue LEU D 501

Chain	Residue
D	THR374
D	TYR375
D	ASN376
D	THR377
D	THR386
D	ARG390
D	GLU451
D	HIS454

---

site_id	AC5
Number of Residues	8
Details	binding site for residue LEU E 501

Chain	Residue
E	THR374
E	TYR375
E	ASN376
E	THR377
E	THR386
E	ARG390
E	GLU451
E	HIS454

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	5
Details	Active site: {"description":"Cysteine sulfenic acid (-SOH) intermediate","evidences":[{"source":"PubMed","id":"26612684","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	25
Details	Binding site: {"evidences":[{"source":"PubMed","id":"26586190","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"5DJ4","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	10
Details	Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)","evidences":[{"source":"PubMed","id":"35114100","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---