5CZZ

Crystal structure of Staphylococcus aureus Cas9 in complex with sgRNA and target DNA (TTGAAT PAM)

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003676	molecular_function	nucleic acid binding
A	0003677	molecular_function	DNA binding
A	0003723	molecular_function	RNA binding
A	0004518	molecular_function	nuclease activity
A	0004519	molecular_function	endonuclease activity
A	0016787	molecular_function	hydrolase activity
A	0043571	biological_process	maintenance of CRISPR repeat elements
A	0046872	molecular_function	metal ion binding
A	0051607	biological_process	defense response to virus

Functional Information from PDB Data

site_id	AC1
Number of Residues	6
Details	binding site for residue NA A 1101

Chain	Residue
A	TYR389
A	THR390
A	HOH1205
A	HOH1223
A	HOH1254
C	HOH201

---

site_id	AC2
Number of Residues	4
Details	binding site for residue NA A 1102

Chain	Residue
A	ILE599
A	LEU592
A	SER594
A	SER597

---

site_id	AC3
Number of Residues	4
Details	binding site for residue NA A 1103

Chain	Residue
A	GLU231
A	MET232
A	MET234
A	HIS236

---

site_id	AC4
Number of Residues	6
Details	binding site for residue NA B 101

Chain	Residue
B	U11
B	G12
B	HOH215
B	HOH216
B	HOH217
C	HOH203

---

site_id	AC5
Number of Residues	1
Details	binding site for residue PO4 B 102

Chain	Residue
B	G70

---

site_id	AC6
Number of Residues	3
Details	binding site for residue EDO B 103

Chain	Residue
B	G21
B	U22
C	DG9

---

site_id	AC7
Number of Residues	6
Details	binding site for residue NA C 101

Chain	Residue
A	HOH1222
C	DT5
C	DC6
C	HOH202
C	HOH204
D	HOH206

---

site_id	AC8
Number of Residues	5
Details	binding site for residue EDO D 101

Chain	Residue
A	ASN885
C	HOH202
D	DG3
D	DA4
D	DA5

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	166
Details	Domain: {"description":"HNH Cas9-type","evidences":[{"source":"HAMAP-Rule","id":"MF_01480","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	385
Details	Region: {"description":"Recognition lobe","evidences":[{"source":"PubMed","id":"26317473","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	46
Details	Region: {"description":"RuvC-II","evidences":[{"source":"PubMed","id":"26317473","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	15
Details	Region: {"description":"PAM substrate-binding","evidences":[{"source":"PubMed","id":"26317473","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	1
Details	Active site: {"description":"For RuvC-like nuclease domain","evidences":[{"source":"PubMed","id":"26317473","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	1
Details	Active site: {"description":"Proton acceptor for HNH nuclease domain","evidences":[{"source":"PubMed","id":"26317473","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	4
Details	Binding site: {"evidences":[{"source":"HAMAP-Rule","id":"MF_01480","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI8
Number of Residues	1
Details	Binding site: {"evidences":[{"source":"PubMed","id":"26317473","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---