5CZZ

Crystal structure of Staphylococcus aureus Cas9 in complex with sgRNA and target DNA (TTGAAT PAM)

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003676	molecular_function	nucleic acid binding
A	0003677	molecular_function	DNA binding
A	0003723	molecular_function	RNA binding
A	0004519	molecular_function	endonuclease activity
A	0043571	biological_process	maintenance of CRISPR repeat elements
A	0046872	molecular_function	metal ion binding
A	0051607	biological_process	defense response to virus

Functional Information from PDB Data

site_id	AC1
Number of Residues	6
Details	binding site for residue NA A 1101

Chain	Residue
A	TYR389
A	THR390
A	HOH1205
A	HOH1223
A	HOH1254
C	HOH201

---

site_id	AC2
Number of Residues	4
Details	binding site for residue NA A 1102

Chain	Residue
A	ILE599
A	LEU592
A	SER594
A	SER597

---

site_id	AC3
Number of Residues	4
Details	binding site for residue NA A 1103

Chain	Residue
A	GLU231
A	MET232
A	MET234
A	HIS236

---

site_id	AC4
Number of Residues	6
Details	binding site for residue NA B 101

Chain	Residue
B	U11
B	G12
B	HOH215
B	HOH216
B	HOH217
C	HOH203

---

site_id	AC5
Number of Residues	1
Details	binding site for residue PO4 B 102

Chain	Residue
B	G70

---

site_id	AC6
Number of Residues	3
Details	binding site for residue EDO B 103

Chain	Residue
B	G21
B	U22
C	DG9

---

site_id	AC7
Number of Residues	6
Details	binding site for residue NA C 101

Chain	Residue
A	HOH1222
C	DT5
C	DC6
C	HOH202
C	HOH204
D	HOH206

---

site_id	AC8
Number of Residues	5
Details	binding site for residue EDO D 101

Chain	Residue
A	ASN885
C	HOH202
D	DG3
D	DA4
D	DA5

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1
Details	ACT_SITE: For RuvC-like nuclease domain => ECO:0000305|PubMed:26317473

Chain	Residue	Details
A	ASP10

---

site_id	SWS_FT_FI2
Number of Residues	1
Details	ACT_SITE: Proton acceptor for HNH nuclease domain => ECO:0000305|PubMed:26317473

Chain	Residue	Details
A	HIS557

---

site_id	SWS_FT_FI3
Number of Residues	4
Details	BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_01480

Chain	Residue	Details
A	ASP10
A	GLU477
A	GLU481
A	HIS701

---

site_id	SWS_FT_FI4
Number of Residues	1
Details	BINDING: BINDING => ECO:0000269|PubMed:26317473

Chain	Residue	Details
A	TYR789

---