5CZZ

Crystal structure of Staphylococcus aureus Cas9 in complex with sgRNA and target DNA (TTGAAT PAM)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-10-26
Detector	PSI PILATUS 6M
Wavelength(s)	1.000
Spacegroup name	P 21 21 2
Unit cell lengths	67.585, 345.584, 98.089
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.044 - 2.600
R-factor	0.2135
Rwork	0.212
R-free	0.24060
Structure solution method	SAD
RMSD bond length	0.002
RMSD bond angle	0.474
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.370	2.660
High resolution limit [Å]	2.600	2.600
Rmerge		0.806
Number of reflections	71486
<I/σ(I)>	14.6	1.9
Completeness [%]	99.4	92.8
Redundancy	6.7	5.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	PEG4000, NaCl, Na2HPO4, NaN3