5CNP

X-ray crystal structure of Spermidine n1-acetyltransferase from Vibrio cholerae.

Replaces:  3EG7

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000287	molecular_function	magnesium ion binding
A	0004145	molecular_function	diamine N-acetyltransferase activity
A	0005737	cellular_component	cytoplasm
A	0006598	biological_process	polyamine catabolic process
A	0016746	molecular_function	acyltransferase activity
A	0016747	molecular_function	acyltransferase activity, transferring groups other than amino-acyl groups
A	0046203	biological_process	spermidine catabolic process
A	0046208	biological_process	spermine catabolic process
A	0046872	molecular_function	metal ion binding
B	0000287	molecular_function	magnesium ion binding
B	0004145	molecular_function	diamine N-acetyltransferase activity
B	0005737	cellular_component	cytoplasm
B	0006598	biological_process	polyamine catabolic process
B	0016746	molecular_function	acyltransferase activity
B	0016747	molecular_function	acyltransferase activity, transferring groups other than amino-acyl groups
B	0046203	biological_process	spermidine catabolic process
B	0046208	biological_process	spermine catabolic process
B	0046872	molecular_function	metal ion binding
C	0000287	molecular_function	magnesium ion binding
C	0004145	molecular_function	diamine N-acetyltransferase activity
C	0005737	cellular_component	cytoplasm
C	0006598	biological_process	polyamine catabolic process
C	0016746	molecular_function	acyltransferase activity
C	0016747	molecular_function	acyltransferase activity, transferring groups other than amino-acyl groups
C	0046203	biological_process	spermidine catabolic process
C	0046208	biological_process	spermine catabolic process
C	0046872	molecular_function	metal ion binding
D	0000287	molecular_function	magnesium ion binding
D	0004145	molecular_function	diamine N-acetyltransferase activity
D	0005737	cellular_component	cytoplasm
D	0006598	biological_process	polyamine catabolic process
D	0016746	molecular_function	acyltransferase activity
D	0016747	molecular_function	acyltransferase activity, transferring groups other than amino-acyl groups
D	0046203	biological_process	spermidine catabolic process
D	0046208	biological_process	spermine catabolic process
D	0046872	molecular_function	metal ion binding
E	0000287	molecular_function	magnesium ion binding
E	0004145	molecular_function	diamine N-acetyltransferase activity
E	0005737	cellular_component	cytoplasm
E	0006598	biological_process	polyamine catabolic process
E	0016746	molecular_function	acyltransferase activity
E	0016747	molecular_function	acyltransferase activity, transferring groups other than amino-acyl groups
E	0046203	biological_process	spermidine catabolic process
E	0046208	biological_process	spermine catabolic process
E	0046872	molecular_function	metal ion binding
F	0000287	molecular_function	magnesium ion binding
F	0004145	molecular_function	diamine N-acetyltransferase activity
F	0005737	cellular_component	cytoplasm
F	0006598	biological_process	polyamine catabolic process
F	0016746	molecular_function	acyltransferase activity
F	0016747	molecular_function	acyltransferase activity, transferring groups other than amino-acyl groups
F	0046203	biological_process	spermidine catabolic process
F	0046208	biological_process	spermine catabolic process
F	0046872	molecular_function	metal ion binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	4
Details	binding site for residue IPA A 201

Chain	Residue
A	ASN53
A	ARG56
A	TYR78
A	TYR109

---

site_id	AC2
Number of Residues	4
Details	binding site for residue MG B 201

Chain	Residue
B	GLU33
B	GLU75
E	GLU33
E	GLU75

---

site_id	AC3
Number of Residues	4
Details	binding site for residue MG D 201

Chain	Residue
C	GLU75
D	GLU33
D	GLU75
C	GLU33

---

site_id	AC4
Number of Residues	6
Details	binding site for residue IPA E 201

Chain	Residue
E	ASN53
E	ARG56
E	TYR78
E	TYR109
E	LEU114
F	MSE28

---

site_id	AC5
Number of Residues	4
Details	binding site for residue MG F 201

Chain	Residue
A	GLU33
A	GLU75
F	GLU33
F	GLU75

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	6
Details	ACT_SITE: Proton donor => ECO:0000250|UniProtKB:P0A951

Chain	Residue	Details
A	TYR134
B	TYR134
C	TYR134
D	TYR134
E	TYR134
F	TYR134

---

site_id	SWS_FT_FI2
Number of Residues	12
Details	BINDING: BINDING => ECO:0000269|PubMed:25623305, ECO:0000269|Ref.3, ECO:0007744|PDB:4MJ8

Chain	Residue	Details
A	MSE28
E	GLU84
F	MSE28
F	GLU84
A	GLU84
B	MSE28
B	GLU84
C	MSE28
C	GLU84
D	MSE28
D	GLU84
E	MSE28

---

site_id	SWS_FT_FI3
Number of Residues	6
Details	BINDING: BINDING => ECO:0000269|PubMed:25623305, ECO:0007744|PDB:4MI4

Chain	Residue	Details
A	GLU33
B	GLU33
C	GLU33
D	GLU33
E	GLU33
F	GLU33

---

site_id	SWS_FT_FI4
Number of Residues	6
Details	BINDING: BINDING => ECO:0000269|PubMed:25623305, ECO:0007744|PDB:4MI4, ECO:0007744|PDB:4R87

Chain	Residue	Details
A	GLU41
B	GLU41
C	GLU41
D	GLU41
E	GLU41
F	GLU41

---

site_id	SWS_FT_FI5
Number of Residues	6
Details	BINDING: BINDING => ECO:0000269|PubMed:25623305, ECO:0007744|PDB:4R87

Chain	Residue	Details
A	HIS49
B	HIS49
C	HIS49
D	HIS49
E	HIS49
F	HIS49

---

site_id	SWS_FT_FI6
Number of Residues	6
Details	BINDING: BINDING => ECO:0000269|PubMed:25623305, ECO:0000269|PubMed:26410587, ECO:0007744|PDB:5CNP

Chain	Residue	Details
A	GLU75
B	GLU75
C	GLU75
D	GLU75
E	GLU75
F	GLU75

---

site_id	SWS_FT_FI7
Number of Residues	12
Details	BINDING: BINDING => ECO:0000269|PubMed:25623305, ECO:0007744|PDB:4R57, ECO:0007744|PDB:4R87

Chain	Residue	Details
A	ILE87
E	GLN94
F	ILE87
F	GLN94
A	GLN94
B	ILE87
B	GLN94
C	ILE87
C	GLN94
D	ILE87
D	GLN94
E	ILE87

---

site_id	SWS_FT_FI8
Number of Residues	6
Details	BINDING: BINDING => ECO:0000269|PubMed:25623305, ECO:0007744|PDB:4R57

Chain	Residue	Details
A	ASN127
B	ASN127
C	ASN127
D	ASN127
E	ASN127
F	ASN127

---

site_id	SWS_FT_FI9
Number of Residues	6
Details	SITE: Could be important for selectivity toward long polyamines => ECO:0000305|PubMed:25623305

Chain	Residue	Details
A	GLU84
B	GLU84
C	GLU84
D	GLU84
E	GLU84
F	GLU84

---