5C7O

Structure of human testis-specific glyceraldehyde-3-phosphate dehydrogenase holo form with NAD+

Functional Information from GO Data

Chain	GOid	namespace	contents
O	0006006	biological_process	glucose metabolic process
O	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
O	0050661	molecular_function	NADP binding
O	0051287	molecular_function	NAD binding
P	0006006	biological_process	glucose metabolic process
P	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
P	0050661	molecular_function	NADP binding
P	0051287	molecular_function	NAD binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	33
Details	binding site for residue NAD O 501

Chain	Residue
O	ASN81
O	LYS151
O	SER169
O	THR170
O	GLY171
O	TYR173
O	SER193
O	ALA194
O	OCS224
O	ALA255
O	ASN388
O	GLY82
O	TYR392
O	SO4502
O	HOH611
O	HOH651
O	HOH666
O	HOH685
O	HOH697
O	HOH737
O	HOH743
O	HOH768
O	PHE83
O	HOH771
O	HOH772
O	HOH778
O	HOH785
O	GLY84
O	ARG85
O	ILE86
O	ASP106
O	PRO107
O	PHE108

---

site_id	AC2
Number of Residues	7
Details	binding site for residue SO4 O 502

Chain	Residue
O	THR254
O	THR256
O	ARG306
O	NAD501
O	HOH611
O	HOH625
O	HOH685

---

site_id	AC3
Number of Residues	33
Details	binding site for residue NAD P 501

Chain	Residue
P	ASN81
P	GLY82
P	PHE83
P	GLY84
P	ARG85
P	ILE86
P	ASP106
P	PRO107
P	PHE108
P	LYS151
P	SER169
P	THR170
P	GLY171
P	TYR173
P	SER193
P	ALA194
P	OCS224
P	ALA255
P	ASN388
P	TYR392
P	SO4502
P	HOH603
P	HOH631
P	HOH638
P	HOH657
P	HOH671
P	HOH677
P	HOH710
P	HOH720
P	HOH730
P	HOH767
P	HOH775
P	HOH783

---

site_id	AC4
Number of Residues	8
Details	binding site for residue SO4 P 502

Chain	Residue
P	THR254
P	THR256
P	ARG306
P	NAD501
P	HOH603
P	HOH654
P	HOH678
P	HOH775

---

Functional Information from PROSITE/UniProt

site_id	PS00071
Number of Residues	8
Details	GAPDH Glyceraldehyde 3-phosphate dehydrogenase active site. ASCTTNcL

Chain	Residue	Details
O	ALA222-LEU229

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	2
Details	Active site: {"description":"Nucleophile","evidences":[{"source":"PROSITE-ProRule","id":"PRU10009","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"21269272","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	12
Details	Binding site: {"evidences":[{"source":"PubMed","id":"21269272","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	6
Details	Binding site: {"evidences":[{"evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	2
Details	Site: {"description":"Activates thiol group during catalysis"}

Chain

Residue

Details

---