5C7O
Structure of human testis-specific glyceraldehyde-3-phosphate dehydrogenase holo form with NAD+
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-04-10 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.0746 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 144.442, 71.741, 80.844 |
Unit cell angles | 90.00, 123.01, 90.00 |
Refinement procedure
Resolution | 31.706 - 1.729 |
R-factor | 0.138 |
Rwork | 0.137 |
R-free | 0.16640 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3h9e |
RMSD bond length | 0.005 |
RMSD bond angle | 1.026 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHENIX |
Refinement software | PHENIX |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 31.710 |
High resolution limit [Å] | 1.730 |
Number of reflections | 68115 |
<I/σ(I)> | 19.2 |
Completeness [%] | 93.9 |
Redundancy | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 273 | 1:1 mixture of protein:NAD+ complex at 10 mg/mL with 0.2 M sodium nitrate, 20% PEG3350 |