5BWX
X-RAY CRYSTAL STRUCTURE AT 1.70A RESOLUTION OF HUMAN MITOCHONDRIAL BRANCHED CHAIN AMINOTRANSFERASE (BCATM) COMPLEXED WITH A 4-CHLORO-2-FLUORO SUBSTITUTED PYRAZOLOPYRIMIDINONE COMPOUND AND AN INTERNAL ALDIMINE LINKED PLP COFACTOR.
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0003824 | molecular_function | catalytic activity |
| A | 0004084 | molecular_function | branched-chain-amino-acid transaminase activity |
| A | 0005515 | molecular_function | protein binding |
| A | 0005654 | cellular_component | nucleoplasm |
| A | 0005739 | cellular_component | mitochondrion |
| A | 0005759 | cellular_component | mitochondrial matrix |
| A | 0006549 | biological_process | isoleucine metabolic process |
| A | 0006550 | biological_process | isoleucine catabolic process |
| A | 0006551 | biological_process | L-leucine metabolic process |
| A | 0006573 | biological_process | valine metabolic process |
| A | 0006629 | biological_process | lipid metabolic process |
| A | 0008483 | molecular_function | transaminase activity |
| A | 0008652 | biological_process | amino acid biosynthetic process |
| A | 0009081 | biological_process | branched-chain amino acid metabolic process |
| A | 0009082 | biological_process | branched-chain amino acid biosynthetic process |
| A | 0009083 | biological_process | branched-chain amino acid catabolic process |
| A | 0009098 | biological_process | L-leucine biosynthetic process |
| A | 0009099 | biological_process | valine biosynthetic process |
| A | 0010817 | biological_process | regulation of hormone levels |
| A | 0050048 | molecular_function | L-leucine:2-oxoglutarate aminotransferase activity |
| A | 0052654 | molecular_function | L-leucine transaminase activity |
| A | 0052655 | molecular_function | L-valine transaminase activity |
| A | 0052656 | molecular_function | L-isoleucine transaminase activity |
| A | 1990830 | biological_process | cellular response to leukemia inhibitory factor |
| B | 0003824 | molecular_function | catalytic activity |
| B | 0004084 | molecular_function | branched-chain-amino-acid transaminase activity |
| B | 0005515 | molecular_function | protein binding |
| B | 0005654 | cellular_component | nucleoplasm |
| B | 0005739 | cellular_component | mitochondrion |
| B | 0005759 | cellular_component | mitochondrial matrix |
| B | 0006549 | biological_process | isoleucine metabolic process |
| B | 0006550 | biological_process | isoleucine catabolic process |
| B | 0006551 | biological_process | L-leucine metabolic process |
| B | 0006573 | biological_process | valine metabolic process |
| B | 0006629 | biological_process | lipid metabolic process |
| B | 0008483 | molecular_function | transaminase activity |
| B | 0008652 | biological_process | amino acid biosynthetic process |
| B | 0009081 | biological_process | branched-chain amino acid metabolic process |
| B | 0009082 | biological_process | branched-chain amino acid biosynthetic process |
| B | 0009083 | biological_process | branched-chain amino acid catabolic process |
| B | 0009098 | biological_process | L-leucine biosynthetic process |
| B | 0009099 | biological_process | valine biosynthetic process |
| B | 0010817 | biological_process | regulation of hormone levels |
| B | 0050048 | molecular_function | L-leucine:2-oxoglutarate aminotransferase activity |
| B | 0052654 | molecular_function | L-leucine transaminase activity |
| B | 0052655 | molecular_function | L-valine transaminase activity |
| B | 0052656 | molecular_function | L-isoleucine transaminase activity |
| B | 1990830 | biological_process | cellular response to leukemia inhibitory factor |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 17 |
| Details | binding site for residue PLP A 401 |
| Chain | Residue |
| A | ARG99 |
| A | VAL269 |
| A | VAL270 |
| A | GLY312 |
| A | THR313 |
| A | 4W4402 |
| A | HOH568 |
| A | HOH669 |
| A | HOH693 |
| A | ARG192 |
| A | LYS202 |
| A | TYR207 |
| A | GLU237 |
| A | THR240 |
| A | ASN242 |
| A | LEU266 |
| A | GLY268 |
| site_id | AC2 |
| Number of Residues | 19 |
| Details | binding site for residue 4W4 A 402 |
| Chain | Residue |
| A | TYR141 |
| A | ARG143 |
| A | VAL182 |
| A | LYS202 |
| A | GLN224 |
| A | VAL238 |
| A | GLY239 |
| A | THR240 |
| A | MET241 |
| A | GLY312 |
| A | ALA314 |
| A | CYS315 |
| A | CYS318 |
| A | PLP401 |
| A | EDO403 |
| A | HOH630 |
| A | HOH674 |
| B | LEU153 |
| B | VAL155 |
| site_id | AC3 |
| Number of Residues | 3 |
| Details | binding site for residue EDO A 403 |
| Chain | Residue |
| A | CYS315 |
| A | 4W4402 |
| A | HOH668 |
| site_id | AC4 |
| Number of Residues | 5 |
| Details | binding site for residue EDO A 404 |
| Chain | Residue |
| A | ASP276 |
| A | THR280 |
| A | GLU348 |
| A | ARG357 |
| A | HIS359 |
| site_id | AC5 |
| Number of Residues | 3 |
| Details | binding site for residue EDO A 405 |
| Chain | Residue |
| A | ARG102 |
| A | PRO267 |
| A | HOH652 |
| site_id | AC6 |
| Number of Residues | 4 |
| Details | binding site for residue EDO A 406 |
| Chain | Residue |
| A | TYR246 |
| A | ARG306 |
| A | ARG344 |
| A | HOH575 |
| site_id | AC7 |
| Number of Residues | 4 |
| Details | binding site for residue EDO A 407 |
| Chain | Residue |
| A | GLU42 |
| A | LEU59 |
| A | THR60 |
| A | HOH594 |
| site_id | AC8 |
| Number of Residues | 4 |
| Details | binding site for residue EDO A 408 |
| Chain | Residue |
| A | GLN234 |
| A | ASN262 |
| A | THR290 |
| A | HOH663 |
| site_id | AC9 |
| Number of Residues | 2 |
| Details | binding site for residue EDO A 409 |
| Chain | Residue |
| A | TRP94 |
| A | HOH736 |
| site_id | AD1 |
| Number of Residues | 6 |
| Details | binding site for residue EDO A 410 |
| Chain | Residue |
| A | ARG106 |
| A | GLY196 |
| A | GLY197 |
| A | HOH619 |
| B | ILE191 |
| B | PRO209 |
| site_id | AD2 |
| Number of Residues | 8 |
| Details | binding site for residue EDO A 411 |
| Chain | Residue |
| A | GLN16 |
| A | TRP194 |
| A | TYR229 |
| A | GLY230 |
| A | HOH512 |
| A | HOH534 |
| A | HOH617 |
| B | TRP194 |
| site_id | AD3 |
| Number of Residues | 2 |
| Details | binding site for residue EDO A 412 |
| Chain | Residue |
| A | GLU216 |
| A | HOH692 |
| site_id | AD4 |
| Number of Residues | 3 |
| Details | binding site for residue EDO A 413 |
| Chain | Residue |
| A | TRP94 |
| A | ASP98 |
| A | LYS114 |
| site_id | AD5 |
| Number of Residues | 7 |
| Details | binding site for residue EDO A 414 |
| Chain | Residue |
| A | ARG102 |
| A | ASN200 |
| A | TYR201 |
| A | CL416 |
| A | HOH604 |
| A | HOH694 |
| A | HOH717 |
| site_id | AD6 |
| Number of Residues | 5 |
| Details | binding site for residue EDO A 415 |
| Chain | Residue |
| A | ASN200 |
| A | LEU261 |
| A | ASN262 |
| A | HOH513 |
| A | HOH717 |
| site_id | AD7 |
| Number of Residues | 2 |
| Details | binding site for residue CL A 416 |
| Chain | Residue |
| A | EDO414 |
| A | HOH513 |
| site_id | AD8 |
| Number of Residues | 4 |
| Details | binding site for residue GOL A 417 |
| Chain | Residue |
| A | PRO260 |
| A | LEU261 |
| A | HOH520 |
| A | HOH660 |
| site_id | AD9 |
| Number of Residues | 18 |
| Details | binding site for residue 4W4 B 402 |
| Chain | Residue |
| B | TYR141 |
| B | ARG143 |
| B | VAL182 |
| B | LYS202 |
| B | GLN224 |
| B | VAL238 |
| B | GLY239 |
| B | THR240 |
| B | MET241 |
| B | GLY312 |
| B | ALA314 |
| B | CYS315 |
| B | CYS318 |
| B | PLP401 |
| B | EDO404 |
| B | HOH640 |
| B | HOH681 |
| A | VAL155 |
| site_id | AE1 |
| Number of Residues | 5 |
| Details | binding site for residue DMS B 403 |
| Chain | Residue |
| B | MET40 |
| B | GLU42 |
| B | LEU59 |
| B | THR60 |
| B | HOH595 |
| site_id | AE2 |
| Number of Residues | 4 |
| Details | binding site for residue EDO B 404 |
| Chain | Residue |
| B | PHE30 |
| B | TYR173 |
| B | 4W4402 |
| B | HOH766 |
| site_id | AE3 |
| Number of Residues | 6 |
| Details | binding site for residue EDO B 405 |
| Chain | Residue |
| B | ASP276 |
| B | THR280 |
| B | GLU348 |
| B | HIS359 |
| B | TRP361 |
| B | HOH520 |
| site_id | AE4 |
| Number of Residues | 5 |
| Details | binding site for residue EDO B 406 |
| Chain | Residue |
| B | TYR246 |
| B | ARG306 |
| B | GLU340 |
| B | ARG344 |
| B | HOH652 |
| site_id | AE5 |
| Number of Residues | 4 |
| Details | binding site for residue EDO B 407 |
| Chain | Residue |
| B | GLU336 |
| B | ASN337 |
| B | HOH551 |
| B | HOH592 |
| site_id | AE6 |
| Number of Residues | 3 |
| Details | binding site for residue GOL B 409 |
| Chain | Residue |
| B | HOH539 |
| B | HOH674 |
| B | HOH718 |
| site_id | AE7 |
| Number of Residues | 21 |
| Details | binding site for Di-peptide PLP B 401 and LYS B 202 |
| Chain | Residue |
| B | LEU74 |
| B | PHE75 |
| B | ARG99 |
| B | SER103 |
| B | ARG192 |
| B | TYR201 |
| B | LEU203 |
| B | TYR207 |
| B | GLU237 |
| B | THR240 |
| B | ASN242 |
| B | LEU266 |
| B | GLY268 |
| B | VAL269 |
| B | VAL270 |
| B | GLY312 |
| B | THR313 |
| B | 4W4402 |
| B | HOH575 |
| B | HOH588 |
| B | HOH659 |
Functional Information from PROSITE/UniProt
| site_id | PS00770 |
| Number of Residues | 35 |
| Details | AA_TRANSFER_CLASS_4 Aminotransferases class-IV signature. EvGtmNIFvywthedgvle.LvTpplngvi.LpGVvR |
| Chain | Residue | Details |
| A | GLU237-ARG271 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 2 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:12269802, ECO:0000269|PubMed:16141215, ECO:0000269|PubMed:17050531 |
| Chain | Residue | Details |
| A | TYR141 | |
| B | TYR141 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 2 |
| Details | MOD_RES: N6-(pyridoxal phosphate)lysine => ECO:0000269|PubMed:16141215, ECO:0000269|PubMed:17050531, ECO:0007744|PDB:2A1H, ECO:0007744|PDB:2HDK, ECO:0007744|PDB:2HG8, ECO:0007744|PDB:2HGW, ECO:0007744|PDB:2HGX, ECO:0007744|PDB:2HHF |
| Chain | Residue | Details |
| A | LYS202 | |
| B | LYS202 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 2 |
| Details | MOD_RES: N6-acetyllysine => ECO:0007744|PubMed:19608861 |
| Chain | Residue | Details |
| A | LYS294 | |
| B | LYS294 |
