5BT4

Crystal structure of BRD4 first bromodomain in complex with SGC-CBP30 chemical probe

Functional Information from PDB Data

site_id	AC1
Number of Residues	11
Details	binding site for residue 2LO A 201

Chain	Residue
A	TRP81
C	GLN127
C	ASP128
A	PRO82
A	LEU92
A	LEU94
A	ASN140
A	HOH305
A	HOH321
C	ASN121
C	GLU124

---

site_id	AC2
Number of Residues	6
Details	binding site for residue EDO A 202

Chain	Residue
A	ILE100
A	ILE101
A	LYS102
A	THR103
A	ASN135
A	HOH320

---

site_id	AC3
Number of Residues	12
Details	binding site for residue 2LO B 201

Chain	Residue
B	TRP81
B	GLN84
C	PRO82
C	PHE83
C	LYS91
C	LEU92
C	LEU94
C	ASN140
C	ILE146
C	2LO201
C	HOH302
C	HOH343

---

site_id	AC4
Number of Residues	4
Details	binding site for residue EDO B 202

Chain	Residue
B	ASP145
B	HOH378
B	HOH419
C	LYS76

---

site_id	AC5
Number of Residues	15
Details	binding site for residue 2LO C 201

Chain	Residue
B	PRO82
B	PHE83
B	LEU92
B	ASN140
B	ILE146
B	2LO201
B	HOH304
B	HOH306
C	GLN78
C	TRP81
C	PRO82
C	GLN84
C	GLN85
C	MET149
C	HOH315

---

Functional Information from PROSITE/UniProt

site_id	PS00633
Number of Residues	60
Details	BROMODOMAIN_1 Bromodomain signature. AwpFqqpvDavklnlpDYYkiIktpMdmgtIkkrlenny..Ywnaqeciqdfnt.MftNCyiY

Chain	Residue	Details
A	ALA80-TYR139

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	318
Details	Domain: {"description":"Bromo 1","evidences":[{"source":"PROSITE-ProRule","id":"PRU00035","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	3
Details	Site: {"description":"Acetylated histone binding","evidences":[{"source":"PubMed","id":"22464331","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	6
Details	Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)","evidences":[{"source":"PubMed","id":"28112733","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

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