5BJ3
THERMUS THERMOPHILUS ASPARTATE AMINOTRANSFERASE TETRA MUTANT 1
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0003824 | molecular_function | catalytic activity |
| A | 0004069 | molecular_function | L-aspartate:2-oxoglutarate aminotransferase activity |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0008483 | molecular_function | transaminase activity |
| A | 0009058 | biological_process | biosynthetic process |
| A | 0030170 | molecular_function | pyridoxal phosphate binding |
| A | 0033853 | molecular_function | aspartate-prephenate aminotransferase activity |
| B | 0003824 | molecular_function | catalytic activity |
| B | 0004069 | molecular_function | L-aspartate:2-oxoglutarate aminotransferase activity |
| B | 0005737 | cellular_component | cytoplasm |
| B | 0008483 | molecular_function | transaminase activity |
| B | 0009058 | biological_process | biosynthetic process |
| B | 0030170 | molecular_function | pyridoxal phosphate binding |
| B | 0033853 | molecular_function | aspartate-prephenate aminotransferase activity |
| C | 0003824 | molecular_function | catalytic activity |
| C | 0004069 | molecular_function | L-aspartate:2-oxoglutarate aminotransferase activity |
| C | 0005737 | cellular_component | cytoplasm |
| C | 0008483 | molecular_function | transaminase activity |
| C | 0009058 | biological_process | biosynthetic process |
| C | 0030170 | molecular_function | pyridoxal phosphate binding |
| C | 0033853 | molecular_function | aspartate-prephenate aminotransferase activity |
| D | 0003824 | molecular_function | catalytic activity |
| D | 0004069 | molecular_function | L-aspartate:2-oxoglutarate aminotransferase activity |
| D | 0005737 | cellular_component | cytoplasm |
| D | 0008483 | molecular_function | transaminase activity |
| D | 0009058 | biological_process | biosynthetic process |
| D | 0030170 | molecular_function | pyridoxal phosphate binding |
| D | 0033853 | molecular_function | aspartate-prephenate aminotransferase activity |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 13 |
| Details | BINDING SITE FOR RESIDUE PLP A 413 |
| Chain | Residue |
| A | GLY99 |
| A | ALA233 |
| A | LYS234 |
| A | ARG242 |
| B | TYR64 |
| A | GLY100 |
| A | SER101 |
| A | TRP125 |
| A | ASN171 |
| A | ASN175 |
| A | ASP203 |
| A | ILE205 |
| A | TYR206 |
| site_id | AC2 |
| Number of Residues | 13 |
| Details | BINDING SITE FOR RESIDUE PLP B 413 |
| Chain | Residue |
| A | TYR64 |
| B | GLY99 |
| B | GLY100 |
| B | SER101 |
| B | TRP125 |
| B | ASN171 |
| B | ASN175 |
| B | ASP203 |
| B | ILE205 |
| B | TYR206 |
| B | ALA233 |
| B | LYS234 |
| B | ARG242 |
| site_id | AC3 |
| Number of Residues | 14 |
| Details | BINDING SITE FOR RESIDUE PLP C 413 |
| Chain | Residue |
| C | GLY99 |
| C | GLY100 |
| C | SER101 |
| C | TRP125 |
| C | ASN171 |
| C | ASN175 |
| C | ASP203 |
| C | ILE205 |
| C | TYR206 |
| C | ALA233 |
| C | LYS234 |
| C | ARG242 |
| C | HOH431 |
| D | TYR64 |
| site_id | AC4 |
| Number of Residues | 15 |
| Details | BINDING SITE FOR RESIDUE PLP D 413 |
| Chain | Residue |
| C | TYR64 |
| C | HOH430 |
| D | GLY99 |
| D | GLY100 |
| D | SER101 |
| D | TRP125 |
| D | ASN171 |
| D | ASN175 |
| D | ASP203 |
| D | ILE205 |
| D | TYR206 |
| D | ALA233 |
| D | LYS234 |
| D | ARG242 |
| D | HOH450 |
Functional Information from PROSITE/UniProt
| site_id | PS00105 |
| Number of Residues | 14 |
| Details | AA_TRANSFER_CLASS_1 Aminotransferases class-I pyridoxal-phosphate attachment site. GAAKafAMtGWRIG |
| Chain | Residue | Details |
| A | GLY231-GLY244 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 4 |
| Details | BINDING: BINDING => ECO:0000250|UniProtKB:P00509 |
| Chain | Residue | Details |
| A | GLY39 | |
| B | GLY39 | |
| C | GLY39 | |
| D | GLY39 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 12 |
| Details | BINDING: BINDING => ECO:0007744|PDB:1GCK |
| Chain | Residue | Details |
| A | TRP125 | |
| D | TRP125 | |
| D | ASN175 | |
| D | ARG361 | |
| A | ASN175 | |
| A | ARG361 | |
| B | TRP125 | |
| B | ASN175 | |
| B | ARG361 | |
| C | TRP125 | |
| C | ASN175 | |
| C | ARG361 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 4 |
| Details | SITE: Important for prephenate aminotransferase activity => ECO:0000269|PubMed:30771275 |
| Chain | Residue | Details |
| A | LYS12 | |
| B | LYS12 | |
| C | LYS12 | |
| D | LYS12 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 4 |
| Details | MOD_RES: N6-(pyridoxal phosphate)lysine => ECO:0000269|PubMed:10029535, ECO:0000269|PubMed:11432784, ECO:0007744|PDB:1B5O, ECO:0007744|PDB:1B5P, ECO:0007744|PDB:5BJ3, ECO:0007744|PDB:5BJ4 |
| Chain | Residue | Details |
| A | LYS234 | |
| B | LYS234 | |
| C | LYS234 | |
| D | LYS234 |
Catalytic Information from CSA
| site_id | CSA1 |
| Number of Residues | 3 |
| Details | Annotated By Reference To The Literature 1ay4 |
| Chain | Residue | Details |
| A | TYR124 | |
| A | ASP203 | |
| B | ALA68 |
| site_id | CSA10 |
| Number of Residues | 3 |
| Details | Annotated By Reference To The Literature 1ay4 |
| Chain | Residue | Details |
| B | LYS234 | |
| B | TYR124 | |
| B | ASP203 |
| site_id | CSA11 |
| Number of Residues | 3 |
| Details | Annotated By Reference To The Literature 1ay4 |
| Chain | Residue | Details |
| C | LYS234 | |
| C | TYR124 | |
| C | ASP203 |
| site_id | CSA12 |
| Number of Residues | 3 |
| Details | Annotated By Reference To The Literature 1ay4 |
| Chain | Residue | Details |
| D | LYS234 | |
| D | TYR124 | |
| D | ASP203 |
| site_id | CSA13 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1ay4 |
| Chain | Residue | Details |
| A | TRP125 | |
| A | ASP203 |
| site_id | CSA14 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1ay4 |
| Chain | Residue | Details |
| B | TRP125 | |
| B | ASP203 |
| site_id | CSA15 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1ay4 |
| Chain | Residue | Details |
| C | TRP125 | |
| C | ASP203 |
| site_id | CSA16 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1ay4 |
| Chain | Residue | Details |
| D | TRP125 | |
| D | ASP203 |
| site_id | CSA17 |
| Number of Residues | 3 |
| Details | Annotated By Reference To The Literature 1ay4 |
| Chain | Residue | Details |
| A | LYS234 | |
| A | ASP203 | |
| A | TYR128 |
| site_id | CSA18 |
| Number of Residues | 3 |
| Details | Annotated By Reference To The Literature 1ay4 |
| Chain | Residue | Details |
| B | LYS234 | |
| B | ASP203 | |
| B | TYR128 |
| site_id | CSA19 |
| Number of Residues | 3 |
| Details | Annotated By Reference To The Literature 1ay4 |
| Chain | Residue | Details |
| C | LYS234 | |
| C | ASP203 | |
| C | TYR128 |
| site_id | CSA2 |
| Number of Residues | 3 |
| Details | Annotated By Reference To The Literature 1ay4 |
| Chain | Residue | Details |
| A | ALA68 | |
| B | TYR124 | |
| B | ASP203 |
| site_id | CSA20 |
| Number of Residues | 3 |
| Details | Annotated By Reference To The Literature 1ay4 |
| Chain | Residue | Details |
| D | LYS234 | |
| D | ASP203 | |
| D | TYR128 |
| site_id | CSA3 |
| Number of Residues | 3 |
| Details | Annotated By Reference To The Literature 1ay4 |
| Chain | Residue | Details |
| D | ALA68 | |
| C | TYR124 | |
| C | ASP203 |
| site_id | CSA4 |
| Number of Residues | 3 |
| Details | Annotated By Reference To The Literature 1ay4 |
| Chain | Residue | Details |
| C | ALA68 | |
| D | TYR124 | |
| D | ASP203 |
| site_id | CSA5 |
| Number of Residues | 3 |
| Details | Annotated By Reference To The Literature 1ay4 |
| Chain | Residue | Details |
| A | LYS234 | |
| A | TRP125 | |
| A | ASP203 |
| site_id | CSA6 |
| Number of Residues | 3 |
| Details | Annotated By Reference To The Literature 1ay4 |
| Chain | Residue | Details |
| B | LYS234 | |
| B | TRP125 | |
| B | ASP203 |
| site_id | CSA7 |
| Number of Residues | 3 |
| Details | Annotated By Reference To The Literature 1ay4 |
| Chain | Residue | Details |
| C | LYS234 | |
| C | TRP125 | |
| C | ASP203 |
| site_id | CSA8 |
| Number of Residues | 3 |
| Details | Annotated By Reference To The Literature 1ay4 |
| Chain | Residue | Details |
| D | LYS234 | |
| D | TRP125 | |
| D | ASP203 |
| site_id | CSA9 |
| Number of Residues | 3 |
| Details | Annotated By Reference To The Literature 1ay4 |
| Chain | Residue | Details |
| A | LYS234 | |
| A | TYR124 | |
| A | ASP203 |
