5BJ3
THERMUS THERMOPHILUS ASPARTATE AMINOTRANSFERASE TETRA MUTANT 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 287 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-02-15 |
| Detector | RIGAKU |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 106.440, 62.250, 154.770 |
| Unit cell angles | 90.00, 109.07, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.200 |
| R-factor | 0.215 |
| Rwork | 0.215 |
| R-free | 0.24300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bjw |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.000 |
| Rmerge | 0.076 | 0.188 |
| Number of reflections | 77082 | |
| <I/σ(I)> | 13.6 | 3 |
| Completeness [%] | 78.8 | 61.9 |
| Redundancy | 2.5 | 1.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.3 | CRYSTALLIZED FROM 30MM AMMONIUM PHOSPHATE, PH 4.3 |
