Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

5A4D

Crystal structure of the chloroplastic gamma-ketol reductase from Arabidopsis thaliana bound to 13KOTE and NADP

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000325	cellular_component	plant-type vacuole
A	0005515	molecular_function	protein binding
A	0005737	cellular_component	cytoplasm
A	0005794	cellular_component	Golgi apparatus
A	0005829	cellular_component	cytosol
A	0005886	cellular_component	plasma membrane
A	0009507	cellular_component	chloroplast
A	0009535	cellular_component	chloroplast thylakoid membrane
A	0009706	cellular_component	chloroplast inner membrane
A	0009941	cellular_component	chloroplast envelope
A	0016491	molecular_function	oxidoreductase activity
B	0000325	cellular_component	plant-type vacuole
B	0005515	molecular_function	protein binding
B	0005737	cellular_component	cytoplasm
B	0005794	cellular_component	Golgi apparatus
B	0005829	cellular_component	cytosol
B	0005886	cellular_component	plasma membrane
B	0009507	cellular_component	chloroplast
B	0009535	cellular_component	chloroplast thylakoid membrane
B	0009706	cellular_component	chloroplast inner membrane
B	0009941	cellular_component	chloroplast envelope
B	0016491	molecular_function	oxidoreductase activity
C	0000325	cellular_component	plant-type vacuole
C	0005515	molecular_function	protein binding
C	0005737	cellular_component	cytoplasm
C	0005794	cellular_component	Golgi apparatus
C	0005829	cellular_component	cytosol
C	0005886	cellular_component	plasma membrane
C	0009507	cellular_component	chloroplast
C	0009535	cellular_component	chloroplast thylakoid membrane
C	0009706	cellular_component	chloroplast inner membrane
C	0009941	cellular_component	chloroplast envelope
C	0016491	molecular_function	oxidoreductase activity
D	0000325	cellular_component	plant-type vacuole
D	0005515	molecular_function	protein binding
D	0005737	cellular_component	cytoplasm
D	0005794	cellular_component	Golgi apparatus
D	0005829	cellular_component	cytosol
D	0005886	cellular_component	plasma membrane
D	0009507	cellular_component	chloroplast
D	0009535	cellular_component	chloroplast thylakoid membrane
D	0009706	cellular_component	chloroplast inner membrane
D	0009941	cellular_component	chloroplast envelope
D	0016491	molecular_function	oxidoreductase activity
E	0000325	cellular_component	plant-type vacuole
E	0005515	molecular_function	protein binding
E	0005737	cellular_component	cytoplasm
E	0005794	cellular_component	Golgi apparatus
E	0005829	cellular_component	cytosol
E	0005886	cellular_component	plasma membrane
E	0009507	cellular_component	chloroplast
E	0009535	cellular_component	chloroplast thylakoid membrane
E	0009706	cellular_component	chloroplast inner membrane
E	0009941	cellular_component	chloroplast envelope
E	0016491	molecular_function	oxidoreductase activity
F	0000325	cellular_component	plant-type vacuole
F	0005515	molecular_function	protein binding
F	0005737	cellular_component	cytoplasm
F	0005794	cellular_component	Golgi apparatus
F	0005829	cellular_component	cytosol
F	0005886	cellular_component	plasma membrane
F	0009507	cellular_component	chloroplast
F	0009535	cellular_component	chloroplast thylakoid membrane
F	0009706	cellular_component	chloroplast inner membrane
F	0009941	cellular_component	chloroplast envelope
F	0016491	molecular_function	oxidoreductase activity
G	0000325	cellular_component	plant-type vacuole
G	0005515	molecular_function	protein binding
G	0005737	cellular_component	cytoplasm
G	0005794	cellular_component	Golgi apparatus
G	0005829	cellular_component	cytosol
G	0005886	cellular_component	plasma membrane
G	0009507	cellular_component	chloroplast
G	0009535	cellular_component	chloroplast thylakoid membrane
G	0009706	cellular_component	chloroplast inner membrane
G	0009941	cellular_component	chloroplast envelope
G	0016491	molecular_function	oxidoreductase activity
H	0000325	cellular_component	plant-type vacuole
H	0005515	molecular_function	protein binding
H	0005737	cellular_component	cytoplasm
H	0005794	cellular_component	Golgi apparatus
H	0005829	cellular_component	cytosol
H	0005886	cellular_component	plasma membrane
H	0009507	cellular_component	chloroplast
H	0009535	cellular_component	chloroplast thylakoid membrane
H	0009706	cellular_component	chloroplast inner membrane
H	0009941	cellular_component	chloroplast envelope
H	0016491	molecular_function	oxidoreductase activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	23
Details	BINDING SITE FOR RESIDUE NAP A 1330

Chain	Residue
A	PRO47
A	THR186
A	GLY188
A	ARG190
A	TYR206
A	CYS228
A	ALA229
A	ASN230
A	ILE250
A	THR251
A	LEU275
A	LYS51
A	LEU277
A	GLY319
A	HIS320
A	ALA321
A	VAL133
A	THR137
A	ALA163
A	SER165
A	GLY166
A	GLY167
A	VAL168

site_id	AC2
Number of Residues	6
Details	BINDING SITE FOR RESIDUE KZH A 1331

Chain	Residue
A	TYR14
A	ARG58
A	PRO59
C	ILE265
D	ARG63
D	LEU100

site_id	AC3
Number of Residues	23
Details	BINDING SITE FOR RESIDUE NAP B 1330

Chain	Residue
B	PRO47
B	LYS51
B	VAL133
B	THR137
B	ALA163
B	SER165
B	GLY166
B	GLY167
B	VAL168
B	THR186
B	GLY188
B	ARG190
B	TYR206
B	CYS228
B	ALA229
B	ASN230
B	ILE250
B	THR251
B	LEU275
B	LEU277
B	GLY319
B	HIS320
B	ALA321

site_id	AC4
Number of Residues	22
Details	BINDING SITE FOR RESIDUE NAP C 1330

Chain	Residue
C	PRO47
C	LYS51
C	VAL133
C	THR137
C	ALA163
C	SER165
C	GLY166
C	GLY167
C	VAL168
C	THR186
C	GLY188
C	ARG190
C	TYR206
C	CYS228
C	ALA229
C	ASN230
C	ILE250
C	THR251
C	LEU275
C	LEU277
C	HIS320
C	ALA321

site_id	AC5
Number of Residues	8
Details	BINDING SITE FOR RESIDUE KZH C 1331

Chain	Residue
A	ILE265
A	THR266
B	PHE60
B	ARG63
B	LEU100
C	TYR14
C	ARG58
C	PRO59

site_id	AC6
Number of Residues	23
Details	BINDING SITE FOR RESIDUE NAP D 1330

Chain	Residue
D	THR251
D	LEU275
D	LEU277
D	GLY319
D	HIS320
D	ALA321
D	PRO47
D	LYS51
D	VAL133
D	THR137
D	ALA163
D	SER165
D	GLY166
D	GLY167
D	VAL168
D	THR186
D	GLY188
D	ARG190
D	TYR206
D	CYS228
D	ALA229
D	ASN230
D	ILE250

site_id	AC7
Number of Residues	6
Details	BINDING SITE FOR RESIDUE KZH D 1331

Chain	Residue
A	LEU100
B	ILE265
B	THR266
D	TYR14
D	ARG58
D	PRO59

site_id	AC8
Number of Residues	20
Details	BINDING SITE FOR RESIDUE NAP E 1330

Chain	Residue
E	PRO47
E	LYS51
E	VAL133
E	THR137
E	ALA163
E	SER165
E	GLY166
E	GLY167
E	VAL168
E	THR186
E	ARG190
E	TYR206
E	ALA229
E	ILE250
E	THR251
E	LEU275
E	LEU277
E	GLY319
E	HIS320
E	ALA321

site_id	AC9
Number of Residues	22
Details	BINDING SITE FOR RESIDUE NAP F 1330

Chain	Residue
F	PRO47
F	LYS51
F	THR137
F	ALA163
F	SER165
F	GLY166
F	GLY167
F	VAL168
F	THR186
F	GLY188
F	ARG190
F	TYR206
F	CYS228
F	ALA229
F	ASN230
F	ILE250
F	THR251
F	LEU275
F	LEU277
F	GLY319
F	HIS320
F	ALA321

site_id	BC1
Number of Residues	5
Details	BINDING SITE FOR RESIDUE KZH F 1331

Chain	Residue
F	TYR14
F	ARG58
F	PRO59
G	ARG63
G	LEU100

site_id	BC2
Number of Residues	23
Details	BINDING SITE FOR RESIDUE NAP G 1330

Chain	Residue
G	PRO47
G	LYS51
G	VAL133
G	THR137
G	ALA163
G	SER165
G	GLY166
G	GLY167
G	VAL168
G	THR186
G	GLY188
G	ARG190
G	TYR206
G	CYS228
G	ALA229
G	ASN230
G	ILE250
G	THR251
G	LEU275
G	LEU277
G	GLY319
G	HIS320
G	ALA321

site_id	BC3
Number of Residues	6
Details	BINDING SITE FOR RESIDUE KZH G 1331

Chain	Residue
E	ILE265
E	THR266
F	PHE60
F	LEU100
G	ARG58
G	PRO59

site_id	BC4
Number of Residues	22
Details	BINDING SITE FOR RESIDUE NAP H 1330

Chain	Residue
H	PRO47
H	LYS51
H	VAL133
H	THR137
H	ALA163
H	SER165
H	GLY166
H	GLY167
H	VAL168
H	THR186
H	ARG190
H	TYR206
H	CYS228
H	ALA229
H	ASN230
H	ILE250
H	THR251
H	LEU275
H	LEU277
H	GLY319
H	HIS320
H	ALA321

site_id	BC5
Number of Residues	3
Details	BINDING SITE FOR RESIDUE KZH H 1331

Chain	Residue
F	THR266
H	ARG58
H	PRO59

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	8
Details	Binding site: {"evidences":[{"source":"Reference","evidenceCode":"ECO:0000269","citation":{"citationType":"submission","publicationDate":"JUN-2015","submissionDatabase":"PDB data bank","title":"Crystal structure of the chloroplastic gamma-ketol reductase from Arabidopsis thaliana.","authors":["Mas-Y-Mas S.","Curien G.","Giustini C.","Rolland N.","Ferrer J.L.","Cobessi D."]}},{"source":"PDB","id":"5A4D","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)