5A4D
Crystal structure of the chloroplastic gamma-ketol reductase from Arabidopsis thaliana bound to 13KOTE and NADP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM30A |
Synchrotron site | ESRF |
Beamline | BM30A |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC QUANTUM 315r |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 82.160, 128.600, 150.120 |
Unit cell angles | 90.00, 97.76, 90.00 |
Refinement procedure
Resolution | 49.581 - 2.807 |
R-factor | 0.1901 |
Rwork | 0.188 |
R-free | 0.22470 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.214 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.580 | 2.980 |
High resolution limit [Å] | 2.810 | 2.810 |
Rmerge | 0.090 | 0.630 |
Number of reflections | 75277 | |
<I/σ(I)> | 13.67 | 2.19 |
Completeness [%] | 99.4 | 98.2 |
Redundancy | 3.8 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 0,2 M SODIUM CHLORIDE, 0,1 M TRIS PH 8,5, 15,5 % PEG 4000 |