4XP3
Crystal structure of ERK2 in complex with an inhibitor
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0000165 | biological_process | MAPK cascade |
| A | 0003690 | molecular_function | double-stranded DNA binding |
| A | 0004672 | molecular_function | protein kinase activity |
| A | 0004674 | molecular_function | protein serine/threonine kinase activity |
| A | 0004707 | molecular_function | MAP kinase activity |
| A | 0005515 | molecular_function | protein binding |
| A | 0005524 | molecular_function | ATP binding |
| A | 0005634 | cellular_component | nucleus |
| A | 0005654 | cellular_component | nucleoplasm |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0005739 | cellular_component | mitochondrion |
| A | 0005769 | cellular_component | early endosome |
| A | 0005770 | cellular_component | late endosome |
| A | 0005794 | cellular_component | Golgi apparatus |
| A | 0005813 | cellular_component | centrosome |
| A | 0005819 | cellular_component | spindle |
| A | 0005829 | cellular_component | cytosol |
| A | 0005856 | cellular_component | cytoskeleton |
| A | 0005886 | cellular_component | plasma membrane |
| A | 0005901 | cellular_component | caveola |
| A | 0005925 | cellular_component | focal adhesion |
| A | 0006357 | biological_process | regulation of transcription by RNA polymerase II |
| A | 0006468 | biological_process | protein phosphorylation |
| A | 0007166 | biological_process | cell surface receptor signaling pathway |
| A | 0008284 | biological_process | positive regulation of cell population proliferation |
| A | 0009636 | biological_process | response to toxic substance |
| A | 0014069 | cellular_component | postsynaptic density |
| A | 0015966 | biological_process | diadenosine tetraphosphate biosynthetic process |
| A | 0016301 | molecular_function | kinase activity |
| A | 0018105 | biological_process | peptidyl-serine phosphorylation |
| A | 0019233 | biological_process | sensory perception of pain |
| A | 0019901 | molecular_function | protein kinase binding |
| A | 0030335 | biological_process | positive regulation of cell migration |
| A | 0030424 | cellular_component | axon |
| A | 0031435 | molecular_function | mitogen-activated protein kinase kinase kinase binding |
| A | 0032355 | biological_process | response to estradiol |
| A | 0032839 | cellular_component | dendrite cytoplasm |
| A | 0032872 | biological_process | regulation of stress-activated MAPK cascade |
| A | 0032991 | cellular_component | protein-containing complex |
| A | 0033574 | biological_process | response to testosterone |
| A | 0035094 | biological_process | response to nicotine |
| A | 0035556 | biological_process | intracellular signal transduction |
| A | 0036120 | biological_process | cellular response to platelet-derived growth factor stimulus |
| A | 0042220 | biological_process | response to cocaine |
| A | 0042307 | biological_process | positive regulation of protein import into nucleus |
| A | 0042542 | biological_process | response to hydrogen peroxide |
| A | 0043204 | cellular_component | perikaryon |
| A | 0043627 | biological_process | response to estrogen |
| A | 0044849 | biological_process | estrous cycle |
| A | 0045727 | biological_process | positive regulation of translation |
| A | 0045880 | biological_process | positive regulation of smoothened signaling pathway |
| A | 0045893 | biological_process | positive regulation of DNA-templated transcription |
| A | 0046697 | biological_process | decidualization |
| A | 0051493 | biological_process | regulation of cytoskeleton organization |
| A | 0060045 | biological_process | positive regulation of cardiac muscle cell proliferation |
| A | 0061431 | biological_process | cellular response to methionine |
| A | 0070371 | biological_process | ERK1 and ERK2 cascade |
| A | 0070849 | biological_process | response to epidermal growth factor |
| A | 0071320 | biological_process | cellular response to cAMP |
| A | 0071364 | biological_process | cellular response to epidermal growth factor stimulus |
| A | 0071375 | biological_process | cellular response to peptide hormone stimulus |
| A | 0071380 | biological_process | cellular response to prostaglandin E stimulus |
| A | 0071396 | biological_process | cellular response to lipid |
| A | 0072584 | biological_process | caveolin-mediated endocytosis |
| A | 0072686 | cellular_component | mitotic spindle |
| A | 0090170 | biological_process | regulation of Golgi inheritance |
| A | 0097237 | biological_process | cellular response to toxic substance |
| A | 0097305 | biological_process | response to alcohol |
| A | 0106310 | molecular_function | protein serine kinase activity |
| A | 1990314 | biological_process | cellular response to insulin-like growth factor stimulus |
| A | 2000641 | biological_process | regulation of early endosome to late endosome transport |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 10 |
| Details | binding site for residue DX4 A 401 |
| Chain | Residue |
| A | ALA50 |
| A | HOH680 |
| A | LYS52 |
| A | GLN103 |
| A | ASP104 |
| A | LEU105 |
| A | MET106 |
| A | LEU154 |
| A | DMS404 |
| A | HOH619 |
| site_id | AC2 |
| Number of Residues | 6 |
| Details | binding site for residue SO4 A 402 |
| Chain | Residue |
| A | TYR185 |
| A | ARG189 |
| A | ARG192 |
| A | TYR231 |
| A | HOH734 |
| A | HOH767 |
| site_id | AC3 |
| Number of Residues | 3 |
| Details | binding site for residue DMS A 403 |
| Chain | Residue |
| A | HIS297 |
| A | GLN304 |
| A | HOH759 |
| site_id | AC4 |
| Number of Residues | 6 |
| Details | binding site for residue DMS A 404 |
| Chain | Residue |
| A | ASP109 |
| A | SER151 |
| A | CYS164 |
| A | DX4401 |
| A | HOH600 |
| A | HOH511 |
| site_id | AC5 |
| Number of Residues | 3 |
| Details | binding site for residue DMS A 405 |
| Chain | Residue |
| A | THR188 |
| A | TRP190 |
| A | HOH788 |
| site_id | AC6 |
| Number of Residues | 2 |
| Details | binding site for residue DMS A 406 |
| Chain | Residue |
| A | LYS149 |
| A | SER151 |
| site_id | AC7 |
| Number of Residues | 3 |
| Details | binding site for residue DMS A 407 |
| Chain | Residue |
| A | SER221 |
| A | PRO224 |
| A | PHE277 |
| site_id | AC8 |
| Number of Residues | 1 |
| Details | binding site for residue DMS A 408 |
| Chain | Residue |
| A | ASN222 |
| site_id | AC9 |
| Number of Residues | 6 |
| Details | binding site for residue DMS A 409 |
| Chain | Residue |
| A | LYS270 |
| A | TRP273 |
| A | LEU285 |
| A | ASP289 |
| A | HOH764 |
| A | HOH644 |
| site_id | AD1 |
| Number of Residues | 10 |
| Details | binding site for residue DMS A 410 |
| Chain | Residue |
| A | HIS118 |
| A | LEU119 |
| A | ILE124 |
| A | LEU220 |
| A | ASN279 |
| A | ALA280 |
| A | HOH756 |
| A | HOH622 |
| A | HOH635 |
| A | HOH530 |
| site_id | AD2 |
| Number of Residues | 5 |
| Details | binding site for residue DMS A 411 |
| Chain | Residue |
| A | ARG77 |
| A | TYR137 |
| A | ALA323 |
| A | GLU324 |
| A | HOH775 |
| site_id | AD3 |
| Number of Residues | 5 |
| Details | binding site for residue DMS A 412 |
| Chain | Residue |
| A | LEU105 |
| A | MET106 |
| A | GLU107 |
| A | ASN156 |
| A | THR157 |
| site_id | AD4 |
| Number of Residues | 5 |
| Details | binding site for residue DMS A 413 |
| Chain | Residue |
| A | ARG75 |
| A | PRO326 |
| A | PHE327 |
| A | LYS328 |
| A | PHE329 |
Functional Information from PROSITE/UniProt
| site_id | PS00107 |
| Number of Residues | 25 |
| Details | PROTEIN_KINASE_ATP Protein kinases ATP-binding region signature. IGEGAYGMVCsAydnlnkvrv.........AIKK |
| Chain | Residue | Details |
| A | ILE29-LYS53 |
| site_id | PS00108 |
| Number of Residues | 13 |
| Details | PROTEIN_KINASE_ST Serine/Threonine protein kinases active-site signature. VlHrDLKpsNLLL |
| Chain | Residue | Details |
| A | VAL143-LEU155 |
| site_id | PS01351 |
| Number of Residues | 103 |
| Details | MAPK MAP kinase signature. FehqtycqrtlREikillrfrheniigindiiraptieqmkdvyivqdlmetdlykllktqhlsndhicyflyqilrglkyihsanvlh..........RDlKpsnlllnttC |
| Chain | Residue | Details |
| A | PHE57-CME159 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 2 |
| Details | Motif: {"description":"TXY"} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 1 |
| Details | Active site: {"description":"Proton acceptor"} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI3 |
| Number of Residues | 9 |
| Details | Binding site: {"evidences":[{"source":"PROSITE-ProRule","id":"PRU00159","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"8639522","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI4 |
| Number of Residues | 1 |
| Details | Modified residue: {"description":"Phosphoserine; by SGK1","evidences":[{"source":"UniProtKB","id":"P28482","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI5 |
| Number of Residues | 1 |
| Details | Modified residue: {"description":"Phosphothreonine","evidences":[{"source":"PubMed","id":"22673903","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI6 |
| Number of Residues | 1 |
| Details | Modified residue: {"description":"Phosphotyrosine","evidences":[{"source":"PubMed","id":"22673903","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI7 |
| Number of Residues | 1 |
| Details | Modified residue: {"description":"Phosphothreonine; by autocatalysis","evidences":[{"source":"UniProtKB","id":"P28482","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI8 |
| Number of Residues | 3 |
| Details | Modified residue: {"description":"Phosphoserine","evidences":[{"source":"UniProtKB","id":"P28482","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
