4XMT
Crystal Structure of Met260Ala mutant of E. coli Aminopeptidase N in complex with L-2,3-Diaminopropionic acid
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0004177 | molecular_function | aminopeptidase activity |
| A | 0005515 | molecular_function | protein binding |
| A | 0005886 | cellular_component | plasma membrane |
| A | 0006508 | biological_process | proteolysis |
| A | 0008233 | molecular_function | peptidase activity |
| A | 0008237 | molecular_function | metallopeptidase activity |
| A | 0008270 | molecular_function | zinc ion binding |
| A | 0016285 | molecular_function | alanyl aminopeptidase activity |
| A | 0016787 | molecular_function | hydrolase activity |
| A | 0042802 | molecular_function | identical protein binding |
| A | 0046872 | molecular_function | metal ion binding |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 4 |
| Details | binding site for residue ZN A 901 |
| Chain | Residue |
| A | HIS297 |
| A | HIS301 |
| A | GLU320 |
| A | DPP902 |
| site_id | AC2 |
| Number of Residues | 14 |
| Details | binding site for residue DPP A 902 |
| Chain | Residue |
| A | GLU298 |
| A | HIS301 |
| A | GLU320 |
| A | TYR381 |
| A | ZN901 |
| A | GOL918 |
| A | HOH1010 |
| A | HOH1175 |
| A | HOH1197 |
| A | GLU121 |
| A | ALA262 |
| A | MET263 |
| A | GLU264 |
| A | HIS297 |
| site_id | AC3 |
| Number of Residues | 6 |
| Details | binding site for residue NA A 903 |
| Chain | Residue |
| A | SER332 |
| A | ASP333 |
| A | GLY335 |
| A | HOH1588 |
| A | HOH1776 |
| A | HOH1877 |
| site_id | AC4 |
| Number of Residues | 6 |
| Details | binding site for residue NA A 904 |
| Chain | Residue |
| A | ASP452 |
| A | NA907 |
| A | HOH1639 |
| A | HOH1885 |
| A | HOH1893 |
| A | HOH1897 |
| site_id | AC5 |
| Number of Residues | 6 |
| Details | binding site for residue NA A 905 |
| Chain | Residue |
| A | GLY56 |
| A | GLY79 |
| A | HOH1393 |
| A | HOH1491 |
| A | HOH1653 |
| A | HOH1718 |
| site_id | AC6 |
| Number of Residues | 5 |
| Details | binding site for residue NA A 906 |
| Chain | Residue |
| A | GLN19 |
| A | ILE20 |
| A | LEU138 |
| A | HOH1728 |
| A | HOH1965 |
| site_id | AC7 |
| Number of Residues | 7 |
| Details | binding site for residue NA A 907 |
| Chain | Residue |
| A | ASP452 |
| A | TYR544 |
| A | NA904 |
| A | HOH1179 |
| A | HOH1844 |
| A | HOH1872 |
| A | HOH1897 |
| site_id | AC8 |
| Number of Residues | 6 |
| Details | binding site for residue NA A 908 |
| Chain | Residue |
| A | HIS771 |
| A | PHE774 |
| A | HOH1191 |
| A | HOH1646 |
| A | HOH1808 |
| A | HOH1865 |
| site_id | AC9 |
| Number of Residues | 4 |
| Details | binding site for residue NA A 909 |
| Chain | Residue |
| A | ASP24 |
| A | HOH1112 |
| A | HOH1279 |
| A | HOH2006 |
| site_id | AD1 |
| Number of Residues | 10 |
| Details | binding site for residue GOL A 910 |
| Chain | Residue |
| A | HIS11 |
| A | ARG14 |
| A | ARG468 |
| A | THR469 |
| A | LYS478 |
| A | GLN479 |
| A | PRO480 |
| A | HOH1049 |
| A | HOH1097 |
| A | HOH1498 |
| site_id | AD2 |
| Number of Residues | 7 |
| Details | binding site for residue GOL A 911 |
| Chain | Residue |
| A | ARG641 |
| A | THR645 |
| A | ARG686 |
| A | ALA722 |
| A | HOH1124 |
| A | HOH1208 |
| A | HOH1445 |
| site_id | AD3 |
| Number of Residues | 5 |
| Details | binding site for residue GOL A 912 |
| Chain | Residue |
| A | LEU532 |
| A | TRP546 |
| A | ASP566 |
| A | ALA567 |
| A | SER570 |
| site_id | AD4 |
| Number of Residues | 9 |
| Details | binding site for residue GOL A 913 |
| Chain | Residue |
| A | SER63 |
| A | VAL64 |
| A | TRP71 |
| A | TRP74 |
| A | ARG669 |
| A | VAL670 |
| A | GLU671 |
| A | HOH1385 |
| A | HOH1517 |
| site_id | AD5 |
| Number of Residues | 4 |
| Details | binding site for residue GOL A 914 |
| Chain | Residue |
| A | GLU292 |
| A | VAL670 |
| A | HIS672 |
| A | HOH1664 |
| site_id | AD6 |
| Number of Residues | 8 |
| Details | binding site for residue GOL A 915 |
| Chain | Residue |
| A | HOH1192 |
| A | HOH1618 |
| A | ARG681 |
| A | GLN703 |
| A | ALA707 |
| A | ASN708 |
| A | ASN709 |
| A | ASP712 |
| site_id | AD7 |
| Number of Residues | 6 |
| Details | binding site for residue GOL A 916 |
| Chain | Residue |
| A | GLU409 |
| A | ASP412 |
| A | SER590 |
| A | LEU591 |
| A | HOH1215 |
| A | HOH1476 |
| site_id | AD8 |
| Number of Residues | 4 |
| Details | binding site for residue GOL A 917 |
| Chain | Residue |
| A | ASP281 |
| A | LYS738 |
| A | TRP739 |
| A | HOH1130 |
| site_id | AD9 |
| Number of Residues | 10 |
| Details | binding site for residue GOL A 918 |
| Chain | Residue |
| A | GLN119 |
| A | GLU121 |
| A | ALA260 |
| A | ASN373 |
| A | TYR376 |
| A | GLN821 |
| A | DPP902 |
| A | HOH1175 |
| A | HOH1356 |
| A | HOH1741 |
| site_id | AE1 |
| Number of Residues | 7 |
| Details | binding site for residue MLI A 919 |
| Chain | Residue |
| A | ALA260 |
| A | GLY261 |
| A | LYS274 |
| A | TYR275 |
| A | ARG783 |
| A | ARG825 |
| A | HOH1156 |
Functional Information from PROSITE/UniProt
| site_id | PS00142 |
| Number of Residues | 10 |
| Details | ZINC_PROTEASE Neutral zinc metallopeptidases, zinc-binding region signature. VIGHEYFHNW |
| Chain | Residue | Details |
| A | VAL294-TRP303 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 1 |
| Details | Active site: {"description":"Proton acceptor","evidences":[{"source":"PubMed","id":"16885166","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"18416562","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"19622865","evidenceCode":"ECO:0000305"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 8 |
| Details | Binding site: {} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI3 |
| Number of Residues | 1 |
| Details | Site: {"description":"Transition state stabilizer","evidences":[{"evidenceCode":"ECO:0000305"}]} |
| Chain | Residue | Details |
