4XMT
Crystal Structure of Met260Ala mutant of E. coli Aminopeptidase N in complex with L-2,3-Diaminopropionic acid
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2014-02-14 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.54 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 120.473, 120.473, 170.620 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 21.140 - 2.000 |
R-factor | 0.13016 |
Rwork | 0.128 |
R-free | 0.16945 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2hpo |
RMSD bond length | 0.021 |
RMSD bond angle | 2.015 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 23.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 92337 | |
<I/σ(I)> | 5.2 | 5.69 |
Completeness [%] | 95.1 | 93.2 |
Redundancy | 5 | 4.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | Sodium malonate |