4X6R
An Isoform-specific Myristylation Switch Targets RIIb PKA Holoenzymes to Membranes
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0000122 | biological_process | negative regulation of transcription by RNA polymerase II |
| A | 0000166 | molecular_function | nucleotide binding |
| A | 0000287 | molecular_function | magnesium ion binding |
| A | 0001669 | cellular_component | acrosomal vesicle |
| A | 0001707 | biological_process | mesoderm formation |
| A | 0001843 | biological_process | neural tube closure |
| A | 0004672 | molecular_function | protein kinase activity |
| A | 0004674 | molecular_function | protein serine/threonine kinase activity |
| A | 0004691 | molecular_function | cAMP-dependent protein kinase activity |
| A | 0004712 | molecular_function | protein serine/threonine/tyrosine kinase activity |
| A | 0005515 | molecular_function | protein binding |
| A | 0005524 | molecular_function | ATP binding |
| A | 0005634 | cellular_component | nucleus |
| A | 0005654 | cellular_component | nucleoplasm |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0005739 | cellular_component | mitochondrion |
| A | 0005813 | cellular_component | centrosome |
| A | 0005829 | cellular_component | cytosol |
| A | 0005886 | cellular_component | plasma membrane |
| A | 0005929 | cellular_component | cilium |
| A | 0005930 | cellular_component | axoneme |
| A | 0005952 | cellular_component | cAMP-dependent protein kinase complex |
| A | 0006397 | biological_process | mRNA processing |
| A | 0006468 | biological_process | protein phosphorylation |
| A | 0006611 | biological_process | protein export from nucleus |
| A | 0007189 | biological_process | adenylate cyclase-activating G protein-coupled receptor signaling pathway |
| A | 0007193 | biological_process | adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway |
| A | 0016020 | cellular_component | membrane |
| A | 0016301 | molecular_function | kinase activity |
| A | 0016607 | cellular_component | nuclear speck |
| A | 0016740 | molecular_function | transferase activity |
| A | 0018105 | biological_process | peptidyl-serine phosphorylation |
| A | 0019901 | molecular_function | protein kinase binding |
| A | 0019904 | molecular_function | protein domain specific binding |
| A | 0021904 | biological_process | dorsal/ventral neural tube patterning |
| A | 0030007 | biological_process | intracellular potassium ion homeostasis |
| A | 0030145 | molecular_function | manganese ion binding |
| A | 0031410 | cellular_component | cytoplasmic vesicle |
| A | 0031514 | cellular_component | motile cilium |
| A | 0031594 | cellular_component | neuromuscular junction |
| A | 0031625 | molecular_function | ubiquitin protein ligase binding |
| A | 0032024 | biological_process | positive regulation of insulin secretion |
| A | 0034237 | molecular_function | protein kinase A regulatory subunit binding |
| A | 0034605 | biological_process | cellular response to heat |
| A | 0036126 | cellular_component | sperm flagellum |
| A | 0042995 | cellular_component | cell projection |
| A | 0044853 | cellular_component | plasma membrane raft |
| A | 0045542 | biological_process | positive regulation of cholesterol biosynthetic process |
| A | 0045667 | biological_process | regulation of osteoblast differentiation |
| A | 0045722 | biological_process | positive regulation of gluconeogenesis |
| A | 0045879 | biological_process | negative regulation of smoothened signaling pathway |
| A | 0046827 | biological_process | positive regulation of protein export from nucleus |
| A | 0048240 | biological_process | sperm capacitation |
| A | 0048471 | cellular_component | perinuclear region of cytoplasm |
| A | 0050766 | biological_process | positive regulation of phagocytosis |
| A | 0050804 | biological_process | modulation of chemical synaptic transmission |
| A | 0051726 | biological_process | regulation of cell cycle |
| A | 0061136 | biological_process | regulation of proteasomal protein catabolic process |
| A | 0070417 | biological_process | cellular response to cold |
| A | 0070613 | biological_process | regulation of protein processing |
| A | 0071333 | biological_process | cellular response to glucose stimulus |
| A | 0071374 | biological_process | cellular response to parathyroid hormone stimulus |
| A | 0071377 | biological_process | cellular response to glucagon stimulus |
| A | 0097546 | cellular_component | ciliary base |
| A | 0098794 | cellular_component | postsynapse |
| A | 0098978 | cellular_component | glutamatergic synapse |
| A | 0099170 | biological_process | postsynaptic modulation of chemical synaptic transmission |
| A | 0106310 | molecular_function | protein serine kinase activity |
| A | 0141156 | biological_process | cAMP/PKA signal transduction |
| A | 1904262 | biological_process | negative regulation of TORC1 signaling |
| A | 1904539 | biological_process | negative regulation of glycolytic process through fructose-6-phosphate |
| A | 1990044 | biological_process | protein localization to lipid droplet |
| A | 2000810 | biological_process | regulation of bicellular tight junction assembly |
| B | 0001932 | biological_process | regulation of protein phosphorylation |
| B | 0005952 | cellular_component | cAMP-dependent protein kinase complex |
| B | 0008603 | molecular_function | cAMP-dependent protein kinase regulator activity |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 2 |
| Details | binding site for residue SO4 A 401 |
| Chain | Residue |
| A | ARG137 |
| A | HOH539 |
| site_id | AC2 |
| Number of Residues | 25 |
| Details | binding site for residue ATP A 402 |
| Chain | Residue |
| A | ALA70 |
| A | LYS72 |
| A | MET120 |
| A | GLU121 |
| A | VAL123 |
| A | GLU127 |
| A | ASP166 |
| A | LYS168 |
| A | GLU170 |
| A | ASN171 |
| A | LEU173 |
| A | THR183 |
| A | ASP184 |
| A | PHE327 |
| A | MG407 |
| A | MG408 |
| B | ARG94 |
| B | ALA97 |
| A | GLY50 |
| A | THR51 |
| A | GLY52 |
| A | SER53 |
| A | PHE54 |
| A | GLY55 |
| A | VAL57 |
| site_id | AC3 |
| Number of Residues | 10 |
| Details | binding site for residue TAM A 403 |
| Chain | Residue |
| A | LYS189 |
| A | ARG194 |
| A | THR195 |
| A | TRP196 |
| A | TPO197 |
| B | TYR103 |
| B | THR104 |
| B | GLU105 |
| B | GLU106 |
| B | HOH610 |
| site_id | AC4 |
| Number of Residues | 5 |
| Details | binding site for residue GOL A 404 |
| Chain | Residue |
| A | ASP329 |
| A | TYR330 |
| A | HOH560 |
| A | HOH567 |
| B | LYS90 |
| site_id | AC5 |
| Number of Residues | 7 |
| Details | binding site for residue MYR A 405 |
| Chain | Residue |
| A | GLY1 |
| A | ASN2 |
| A | PHE18 |
| A | LEU152 |
| A | GLU155 |
| A | GLN307 |
| A | LYS309 |
| site_id | AC6 |
| Number of Residues | 4 |
| Details | binding site for residue SO4 A 406 |
| Chain | Residue |
| A | ARG194 |
| A | ASN216 |
| A | LYS217 |
| A | HOH561 |
| site_id | AC7 |
| Number of Residues | 3 |
| Details | binding site for residue MG A 407 |
| Chain | Residue |
| A | ASN171 |
| A | ASP184 |
| A | ATP402 |
| site_id | AC8 |
| Number of Residues | 3 |
| Details | binding site for residue MG A 408 |
| Chain | Residue |
| A | PHE54 |
| A | ASP184 |
| A | ATP402 |
| site_id | AC9 |
| Number of Residues | 11 |
| Details | binding site for residue SO4 B 401 |
| Chain | Residue |
| B | PHE322 |
| B | GLY323 |
| B | GLU324 |
| B | ILE325 |
| B | HOH548 |
| B | HOH550 |
| B | HOH551 |
| B | HOH579 |
| B | HOH582 |
| B | HOH619 |
| B | HOH621 |
| site_id | AD1 |
| Number of Residues | 5 |
| Details | binding site for residue SO4 B 402 |
| Chain | Residue |
| A | PRO258 |
| A | SER259 |
| B | GLN283 |
| B | GLY284 |
| B | ARG303 |
| site_id | AD2 |
| Number of Residues | 7 |
| Details | binding site for residue SO4 B 403 |
| Chain | Residue |
| B | ILE163 |
| B | GLY169 |
| B | ASP170 |
| B | ASN171 |
| B | TYR173 |
| B | ARG209 |
| B | HOH567 |
| site_id | AD3 |
| Number of Residues | 6 |
| Details | binding site for residue SO4 B 404 |
| Chain | Residue |
| B | ARG93 |
| B | MET329 |
| B | ASN330 |
| B | ARG350 |
| B | HOH505 |
| B | HOH509 |
| site_id | AD4 |
| Number of Residues | 6 |
| Details | binding site for residue GOL B 405 |
| Chain | Residue |
| A | LYS83 |
| B | SER99 |
| B | ALA100 |
| B | GLU101 |
| B | GLY206 |
| B | ARG226 |
Functional Information from PROSITE/UniProt
| site_id | PS00107 |
| Number of Residues | 24 |
| Details | PROTEIN_KINASE_ATP Protein kinases ATP-binding region signature. LGTGSFGRVMlVkhkesgnh..........YAMK |
| Chain | Residue | Details |
| A | LEU49-LYS72 |
| site_id | PS00108 |
| Number of Residues | 13 |
| Details | PROTEIN_KINASE_ST Serine/Threonine protein kinases active-site signature. LiYrDLKpeNLLI |
| Chain | Residue | Details |
| A | LEU162-ILE174 |
| site_id | PS00888 |
| Number of Residues | 17 |
| Details | CNMP_BINDING_1 Cyclic nucleotide-binding domain signature 1. VIqQGDeGDnFYVIdqG |
| Chain | Residue | Details |
| B | VAL162-GLY178 | |
| B | ILE280-GLY296 |
| site_id | PS00889 |
| Number of Residues | 18 |
| Details | CNMP_BINDING_2 Cyclic nucleotide-binding domain signature 2. FGElALiygtp......RAAtVkA |
| Chain | Residue | Details |
| B | PHE198-ALA215 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 6 |
| Details | BINDING: |
| Chain | Residue | Details |
| B | LEU135 | |
| B | GLU200 | |
| B | ARG209 | |
| B | ILE253 | |
| B | GLU324 | |
| B | LYS333 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 1 |
| Details | MOD_RES: Phosphoserine => ECO:0000250|UniProtKB:Q9DBC7 |
| Chain | Residue | Details |
| B | SER99 | |
| A | LYS72 | |
| A | GLU121 | |
| A | LYS168 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 1 |
| Details | MOD_RES: Phosphoserine => ECO:0000250|UniProtKB:P09456 |
| Chain | Residue | Details |
| B | SER256 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 1 |
| Details | MOD_RES: Phosphoserine; by autocatalysis => ECO:0000269|PubMed:11141074, ECO:0000269|PubMed:8395513 |
| Chain | Residue | Details |
| A | SER10 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 2 |
| Details | MOD_RES: Phosphothreonine => ECO:0000250|UniProtKB:P17612 |
| Chain | Residue | Details |
| A | THR48 | |
| A | THR195 |
| site_id | SWS_FT_FI6 |
| Number of Residues | 1 |
| Details | MOD_RES: Phosphoserine => ECO:0000269|PubMed:22323819, ECO:0000305|PubMed:8395513 |
| Chain | Residue | Details |
| A | SEP139 |
| site_id | SWS_FT_FI7 |
| Number of Residues | 1 |
| Details | MOD_RES: Phosphothreonine; by PDPK1 => ECO:0000269|PubMed:22323819, ECO:0000269|PubMed:8395513, ECO:0000269|PubMed:9707564 |
| Chain | Residue | Details |
| A | TPO197 |
| site_id | SWS_FT_FI8 |
| Number of Residues | 1 |
| Details | MOD_RES: Phosphotyrosine => ECO:0000269|PubMed:21866565 |
| Chain | Residue | Details |
| A | TYR330 |
| site_id | SWS_FT_FI9 |
| Number of Residues | 1 |
| Details | MOD_RES: Phosphoserine => ECO:0000305|PubMed:8395513 |
| Chain | Residue | Details |
| A | SEP338 |
| site_id | SWS_FT_FI10 |
| Number of Residues | 1 |
| Details | LIPID: N-myristoyl glycine => ECO:0000269|PubMed:11141074 |
| Chain | Residue | Details |
| A | GLY1 |
