4X5I
ecDHFR complexed with folate and NADP+ at 660 MPa
Functional Information from GO Data
Chain | GOid | namespace | contents |
A | 0004146 | molecular_function | dihydrofolate reductase activity |
A | 0005515 | molecular_function | protein binding |
A | 0005542 | molecular_function | folic acid binding |
A | 0005829 | cellular_component | cytosol |
A | 0006730 | biological_process | one-carbon metabolic process |
A | 0009257 | biological_process | 10-formyltetrahydrofolate biosynthetic process |
A | 0009410 | biological_process | response to xenobiotic stimulus |
A | 0016491 | molecular_function | oxidoreductase activity |
A | 0031427 | biological_process | response to methotrexate |
A | 0046452 | biological_process | dihydrofolate metabolic process |
A | 0046654 | biological_process | tetrahydrofolate biosynthetic process |
A | 0046655 | biological_process | folic acid metabolic process |
A | 0046656 | biological_process | folic acid biosynthetic process |
A | 0046677 | biological_process | response to antibiotic |
A | 0050661 | molecular_function | NADP binding |
A | 0051870 | molecular_function | methotrexate binding |
A | 0051871 | molecular_function | dihydrofolic acid binding |
A | 0070401 | molecular_function | NADP+ binding |
A | 0070402 | molecular_function | NADPH binding |
Functional Information from PDB Data
site_id | AC1 |
Number of Residues | 15 |
Details | binding site for residue FOL A 201 |
Chain | Residue |
A | ILE5 |
A | ARG57 |
A | ILE94 |
A | TYR100 |
A | THR113 |
A | HOH370 |
A | HOH511 |
A | ALA6 |
A | ALA7 |
A | ASP27 |
A | TRP30 |
A | PHE31 |
A | LYS32 |
A | ILE50 |
A | ARG52 |
site_id | AC2 |
Number of Residues | 30 |
Details | binding site for residue NAP A 202 |
Chain | Residue |
A | GLY43 |
A | ARG44 |
A | HIS45 |
A | THR46 |
A | LEU62 |
A | SER63 |
A | SER64 |
A | ASP70 |
A | ARG71 |
A | VAL72 |
A | LYS76 |
A | GLY96 |
A | GLY97 |
A | ARG98 |
A | VAL99 |
A | GLN102 |
A | ASP122 |
A | THR123 |
A | HOH301 |
A | HOH305 |
A | HOH313 |
A | HOH330 |
A | HOH332 |
A | HOH339 |
A | HOH381 |
A | HOH405 |
A | HOH410 |
A | HOH427 |
A | HOH507 |
A | HOH508 |
site_id | AC3 |
Number of Residues | 4 |
Details | binding site for residue BME A 203 |
Chain | Residue |
A | SER138 |
A | CYS152 |
A | PHE153 |
A | GLU154 |
Functional Information from PROSITE/UniProt
site_id | PS00075 |
Number of Residues | 23 |
Details | DHFR_1 Dihydrofolate reductase (DHFR) domain signature. VIGmenaMPWnlpa.DlawFkrnT |
Chain | Residue | Details |
A | VAL13-THR35 |
Functional Information from SwissProt/UniProt
site_id | SWS_FT_FI1 |
Number of Residues | 5 |
Details | BINDING: BINDING => ECO:0000305|PubMed:9012674 |
Chain | Residue | Details |
A | ILE5 | |
A | ASP27 | |
A | ARG52 | |
A | ARG57 | |
A | THR113 |
site_id | SWS_FT_FI2 |
Number of Residues | 6 |
Details | BINDING: BINDING => ECO:0000269|PubMed:19374017 |
Chain | Residue | Details |
A | ALA7 | |
A | VAL13 | |
A | HIS45 | |
A | SER63 | |
A | LYS76 | |
A | GLY95 |