4X5I

ecDHFR complexed with folate and NADP+ at 660 MPa

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	298
Detector technology	CCD
Collection date	2013-02-12
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.71
Spacegroup name	C 1 2 1
Unit cell lengths	73.491, 58.520, 38.114
Unit cell angles	90.00, 107.01, 90.00

Refinement procedure

Resolution	44.970 - 1.800
R-factor	0.17408
Rwork	0.171
R-free	0.24049
RMSD bond length	0.018
RMSD bond angle	2.082
Data scaling software	HKL-2000
Refinement software	REFMAC (5.7.0032)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.800
Number of reflections	14675
<I/σ(I)>	16.8
Completeness [%]	95.1
Redundancy	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	PEG 6000, imidazole, calcium chloride